| 赵秀英,符之富,阙利双,符宗军,李文华.血浆miR-106b、miR-146a表达水平与癫痫患儿脑电图参数、Th17细胞和凋亡分子的相关性分析[J].,2021,(17):3378-3382 |
| 血浆miR-106b、miR-146a表达水平与癫痫患儿脑电图参数、Th17细胞和凋亡分子的相关性分析 |
| Correlation Analysis of Plasma miR-106b and miR-146a Expression Levels with Electroencephalogram Parameters, Th17 Cells and Apoptotic Molecules in Children with Epilepsy |
| 投稿时间：2021-03-03 修订日期：2021-03-27 |
| DOI：10.13241/j.cnki.pmb.2021.17.038 |
| 中文关键词: 癫痫 脑电图 辅助性T细胞17 细胞凋亡 miR-106b miR-146a |
| 英文关键词: Epilepsy Electroencephalogram Helper T cell 17 Apoptosis miR-106 b miR-146a |
| 基金项目:海南省卫生计生行业科研项目（16A200041） |
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| 中文摘要: |
| 摘要 目的：分析血浆miR-106b、miR-146a表达特点及其与脑电图参数、辅助性T细胞17（Th17）和凋亡分子的相关性以及诊断癫痫的价值。方法：选择2018年1月至2020年10月我院收治的癫痫患儿75例作为癫痫组，检测受试者血浆miR-106b、miR-146a表达，外周血Th17细胞占比、血清B细胞淋巴瘤/白血病-1（Bcl-1）、BCL2-Associated X蛋白（Bax）、Survivin、半胱氨酸天冬酰胺酶（Caspase-3）水平和脑电图参数α、β、 δ、θ波功率。分析miR-106b、miR-146a与Th17细胞占比、Bcl-1、Bax、Survivin、Caspase-3以及α、β、 δ、θ波功率的相关性，受试者工作特征（ROC）曲线分析miR-106b、miR-146a诊断癫痫的价值。结果：癫痫组血浆miR-106b、miR-146a表达、Th17细胞占比、Bax、Caspase-3水平高于对照组（P＜0.05），α波功率、θ波功率、Bcl-1、Survivin水平低于对照组（P＜0.05）。miR-106b、miR-146a表达与Th17细胞占比、Bax、Caspase-3呈正相关（P＜0.05），与α波功率、θ波功率、Bcl-1、Survivin呈负相关（P＜0.05）。联合miR-106b和miR-146a诊断癫痫的曲线下面积（AUC）为0.975，高于单独miR-106b和miR-146a诊断的0.884、0.835。结论：癫痫患儿血浆miR-146a、miR-106b表达增高，miR-146a、miR-106b高表达与脑电图异常、Th17细胞功能障碍以及神经细胞凋亡有关，miR-146a、miR-106b有望成为癫痫诊断的新生物学标志物。 |
| 英文摘要: |
| ABSTRACT Objective: To analyze the expression characteristics of plasma miR-106b and miR-146a, and to analyze the correlation between miR-106b and miR-146a and electroencephalogram parameters, helper T cell 17(Th17) and apoptosis molecules, as well as their diagnostic value in epilepsy. Methods: A total of 75 children with epilepsy admitted to our hospital from January 2018 to October 2020 were selected as the epilepsy group. The expression of plasma miR-106b and miR-146a of the subjects were detected, the proportion of Th17 cells in peripheral blood, the levels of serum B-cell lymphoma / leukemia-1(Bcl-1), BCL2-Associated X protein (Bax), Survivin, cysteine asparaginase(Caspase-3) and electroencephalogram parameters α, β, δ, θ wave power were measured. The correlation between miR-106b, miR-146a and the proportion of Th17 cells, Bcl-1, Bax, Survivin, Caspase-3, α, β, δ, θ wave power were analyzed. The value of miR-106b and miR-146a in the diagnosis of epilepsy was analyzed by receiver operating characteristic (ROC) curve. Results: The expressions of plasma miR-106b and miR-146a, the proportion of Th17 cells, the levels of serum Bax and Caspase-3 in epilepsy group were higher than those in control group(P＜0.05), and the α wave power, θ wave power, the levels of Bcl-1 and Survivin were lower than those in control group(P＜0.05). The expression of miR-106b and miR-146a were positively correlated with the proportion of Th17 cells, Bax and Caspase-3(P＜0.05), and negatively correlated with α wave power, θ wave power, Bcl-1 and Survivin(P＜0.05). The area under curve(AUC) of combined miR-106b and miR-146a in the diagnosis of epilepsy was 0.975, which was higher than that of 0.884 and 0.835 of miR-106b and miR-146a alone. Conclusion: The expression of plasma miR-146a and miR-106b of children with epilepsy is increased, and the high expression of miR-146a and miR-106b are related to abnormal electroencephalogram, Th17 cell dysfunction and nerve cell apoptosis, miR-146a and miR-106b are expected to be new biomarkers for the diagnosis of epilepsy. |
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