| 梁 静,凯赛尔江·多来提,范 娜,刘 雯,古丽美热·艾买如拉,郑淑贤.IL-10在输血相关性移植物抗宿主病小鼠模型中的免疫调节作用[J].,2020,(1):55-58 |
| IL-10在输血相关性移植物抗宿主病小鼠模型中的免疫调节作用 |
| Immunomodulatory Effect of IL-10 in Transfusion-associated Graft-versus-host Disease Mouse Model |
| 投稿时间：2019-07-18 修订日期：2019-08-14 |
| DOI：10.13241/j.cnki.pmb.2020.01.011 |
| 中文关键词: IL-10 输血相关性移植物抗宿主病 免疫抑制 |
| 英文关键词: IL-10 TA-GVHD Immunosuppression |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2016D01C214） |
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| 中文摘要: |
| 摘要 目的：IL-10在输血相关性移植物抗宿主病小鼠模型中的免疫调节作用。方法：取BALB／c实验小鼠免疫活性淋巴细胞，分别输注于BALB／c小鼠（设为A组）及BALB／c裸鼠（设为B组），建立TA-GVHD模型，观察小鼠症状，HE染色判断小鼠肝、肺、小肠、皮肤病理变化情况；采用双夹心酶联免疫吸附法（ELISA）检测两组小鼠血清IL-10浓度；用逆转录聚合酶链反应法RT-PCR检测移植后外周血单个核细胞中IL-10的表达。结果：A组中2只死亡（12.5%），B组中3只死亡（18.75%），共5只死亡，29只存活，两组死亡率比较无明显差异（P＞0.05）。B组小鼠累及肝、肺、小肠和皮肤病理损伤程度较A组严重；存活小鼠IL-10浓度较死亡小鼠明显升高（P2＜0.05）；存活小鼠IL-10 mRNA表达阳性率96.55%明显高于死亡小鼠（20.00%）。结论：IL-10在输血相关的移植物抗宿主病小鼠模型中发挥负向免疫调节--免疫抑制作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the immunomodulatory effect of IL-10 on mice with transfusion-associated graft-versus-host disease (TA-GVHD). Methods: The immune active lymphocytes of BALB/c experimental mice were infused into BALB/c mice (group A) and BALB/c nude mice (group B), respectively. the TA-GVHD model was established and the symptoms of mice were observed. HE staining was used to determine the pathological changes of liver, lung, small intestine and skin of mice. The concentration of serum IL-10 in the two groups was detected by double sandwich enzyme-linked immunosorbent assay (ELISA), and the expression of IL-10 in peripheral blood mononuclear cells (PBMCs) after transplantation was detected by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Two rats died in group A (12.5%), B group, 3 died (18.75%), 5 died and 29 survived. there was no significant difference in mortality between the two groups (P > 0.05). The pathological damage of liver, lung, small intestine and skin in group B was more serious than that in group A; The concentration of IL-10 in surviving mice was significantly higher than that in dead mice (P < 0.05), and the positive rate of IL-10mRNA expression in surviving mice was 96.55%, which was significantly higher than that in dead mice (20.00%). Conclusion: IL-10 may play an immunosuppressive role in the mouse model of TA-GVHD. |
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