| 何 志,李学拥,雷战军,李 靖,黄 辞.改良皮片4℃低温保存方法在创面二次植皮治疗中的应用研究[J].,2019,19(17):3230-3233 |
| 改良皮片4℃低温保存方法在创面二次植皮治疗中的应用研究 |
| A Study on the Application of Improved Skin Cryopreservation Technique at 4 ℃ for the Wounds Needing Secondary Skin-grafting |
| 投稿时间：2019-04-12 修订日期：2019-05-15 |
| DOI：10.13241/j.cnki.pmb.2019.17.006 |
| 中文关键词: 低温皮肤保存 4℃ 皮肤活力 |
| 英文关键词: Skin Cryopreservation Technique 4℃ The Skin Viability |
| 基金项目:国家自然科学基金项目（81272134） |
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| 中文摘要: |
| 摘要 目的：探讨改良皮片4℃低温保存方法在创面二次植皮治疗中的应用效果。方法：①动物实验：健康成年豚鼠3只，处死后，取背部皮肤做成32个1 cm×1 cm小皮样，随机分为新鲜皮组、庆大盐水保存组、RPMI保存组，改良RPMI保存组进行4℃保存；1周后测定皮肤活力；②临床实验：观察自2018年10月至2018年12月，二期植皮患者33例，应用改良RPMI 4℃低温保存皮肤二期回植的患者16例，与重新取皮植皮患者17例比较其皮片成活率。结果：动物实验证实：改良RPMI保存组4℃保存组在皮肤储存1周时皮肤平均活力较庆大盐水保存组、RPMI保存组高（P<0.05）；临床实验证实：改良RPMI保存组与重新植皮的皮片平均成活率没有显著性差别（P>0.05）。结论：改良4℃断层皮片低温保存方法可短期内保存皮肤较高的活力，是皮片再利用的一种有效方法。 |
| 英文摘要: |
| ABSTRACT Objective: To study the application value of modified 4℃ skin cryopreservation technique for the wound needing second skin grafting. Methods: ①Animal experiment: 3 healthy adult guinea pigs were sacrificed. 32 1 cm×1 cm pieces of skin taken from the back were randomly divided into 4 groups: the fresh skin group, Gentamicin-brine group, RPMI group, improved RPMI group. After 1 week, the viability of skins were measured. ②Clinical experiment: 16 cases of patients needking secondary skin-grafting from October 2018 to December 2018 were selected, 16 cases were griven improved RPMI 4℃ cryopreservation and the other 17 cases were given needing skin-grafting. The skin-grafting survival rates were evaluated. Results: Animal experiment: the average viability of skins in improved RPMI 4℃ cryopreservation group were higher than the Gentamicin-brine group and RPMI group after 1 week (P<0.05). Clinical experiment: there was no significant difference in the skin-grafting survival rate between the improved RPMI cryopreservation group and the new skin-grafting group (P>0.05). Conclusion: Improved RPMI 4℃ cryopreservation technique is an effective method to reuse the skins by keeping the high viability for a short time. |
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