| 吴文林,钟永怡,周 泉,唐 杰,王家骥.早孕因子单克隆抗体对黑色素瘤细胞A-375增殖、凋亡的影响[J].,2019,19(5):820-823 |
| 早孕因子单克隆抗体对黑色素瘤细胞A-375增殖、凋亡的影响 |
| Effect of Monoclonal Antibody against Early Pregnancy Factor on Proliferation and Apoptosis of Melanoma cell A-375 |
| 投稿时间：2018-07-07 修订日期：2018-07-30 |
| DOI：10.13241/j.cnki.pmb.2019.05.005 |
| 中文关键词: 早孕因子 单克隆抗体 黑色素瘤 |
| 英文关键词: Early pregnancy factor Monoclonal antibody Melanoma |
| 基金项目:广东省自然科学基金项目（2015A030313455） |
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| 中文摘要: |
| 摘要 目的：研究早孕因子单克隆抗体（EPF-McAb）对黑色素瘤细胞A-375增殖、凋亡的影响。方法：体外培养黑色素瘤细胞A-375，通过CCK-8实验检测EPF-McAb对A-375细胞增殖的影响；通过流式细胞术检测EPF-McAb对A-375细胞凋亡的影响；Western Blot检测EPF-McAb对A-375细胞EPF蛋白表达的影响。结果：CCK-8结果显示EPF-McAb可以抑制黑色素瘤细胞A-375的增殖，且随着作用时间延长和药物浓度的增加，对A-375细胞增殖的抑制作用也增强；流式细胞术实验结果显示EPF-McAb可以促进黑色素瘤细胞A-375的凋亡，且调亡率随着药物浓度的增加而升高，0.2、0.4、0.8 mg/mL的EPF单抗作用于A-375细胞24 h后，细胞调亡率分别为：14.68 %(P<0.01)、19.81 %(P<0.01)、23.97 %(P<0.01)；Western Blot实验结果显示EPF-McAb可以降低黑色素瘤细胞A-375 EPF蛋白的表达。结论：早孕因子单克隆抗体可以抑制黑色素瘤细胞A-375的增殖，并促进其凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effect of early pregnancy factor monoclonal antibody (EPF-McAb) on proliferation and apop- tosis of melanoma cell A-375. Methods: CCK-8 experiment, flow cytometry and Western Blot were conducted on cultured melanoma cells A-375 in vitro to understand the effect of EPF-McAb on proliferation, apoptosis and protein expression of A-375 cells respectively. Results: CCK-8 results showed that EPF-McAb could inhibit the proliferation of A-375 in melanoma cells, and the effect was also en- hanced with the prolongation of action time and the increase of drug concentration. The results of flow cytometry showed that EPF-McAb could promote the apoptosis of A-375 in melanoma cells, and the rate of apoptosis was associated with the drug concentra- tion. The increase of 0.2, 0.4, 0.8 mg/mL EPF monoclonal antibody on A-375 cells 24 h, the cell apoptosis rate was 14.68% (P<0.01), 19.81% (P<0.01), 23.97% (P<0.01); Western Blot experimental results showed that EPF-McAb can reduce the expression of A-375 EPF protein in melanoma cells. Conclusion: EPF-McAb can not only inhibit the proliferation of melanoma cell A-375, but also promote its apoptosis. |
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