| 周金爱,余 军,黄东萍,吴 睿,刘 欣,臧 宁.速生桉叶水溶提取物对HepG2细胞增殖及凋亡的影响[J].,2018,(24):4623-4627 |
| 速生桉叶水溶提取物对HepG2细胞增殖及凋亡的影响 |
| Effects of Leaf Water Extract from Fast-Growing Eucylyptus Robusta on Proliferaton and Apoptosis of Human Heptoma HepG2 Cells |
| 投稿时间：2018-06-21 修订日期：2018-07-26 |
| DOI：10.13241/j.cnki.pmb.2018.24.004 |
| 中文关键词: 速生桉 HepG2 细胞增殖 细胞凋亡 |
| 英文关键词: Fast-growing eucylyptus HepG2 Cells Proliferation Apoptosis |
| 基金项目:广西科学研究与技术开发计划项目（桂科能1598025-12）；广西研究生教育创新计划项目（YCSW2018118） |
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| 中文摘要: |
| 摘要 目的：探讨速生桉叶水溶提取物对HepG2细胞的抑制增殖作用及凋亡的影响。方法：采用MTT法检测速生桉叶水溶提取物对HepG2细胞增殖抑制作用；采用流式细胞仪Annexin V-FITC/PI检测速生桉叶水溶提取物处理后HepG2细胞凋亡的情况。结果：MTT分析显示，当细胞培养24 h时，稀释5、10倍HepG2细胞抑制率分别为（47.32±1.11）%、（15.76±3.50）%；当细胞培养48 h时，稀释5、10倍HepG2细胞抑制率分别为（44.13±10.93）%、（25.93±8.37）%；当细胞培养72 h时，稀释5、10倍HepG2细胞抑制率分别为（59.47±6.90）%、（41.02±4.27）%。流式细胞术结果显示，速生桉叶水溶提取物稀释30倍时可诱导31.03%的HepG2细胞进入早期凋亡阶段，随着稀释倍数的减少被诱导进入凋亡阶段的细胞数目逐渐升高。另外，随着水溶提取物作用时间的延长，进入凋亡阶段的细胞数目也逐渐升高。结论：速生桉叶水溶提取物对HepG2细胞增殖与凋亡具有量-效、时-效关系。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of the leaf of Fast-growing eucylyptus extract on the proliferation and apoptosis of human hepatoma HepG2 cells. Methods: MTT assay was used to measure the leaf of Fast-growing eucylyptus extract on HepG2 cells activity. The cell apoptosis of HepG2 cells were determined by flow cytometry Annexin V-FITC/PI. Results: As the results of MTT showed, HepG2 cells were cultured for 24 h, the inhibition rates of HepG2 cells diluted 5 and 10 times were (47.32±1.11) % and (15.76 ±3.50) %, respectively. When HepG2 cells were cultured for 48 h, the inhibition rates of HepG2 cells diluted 5 and 10 times were respectively (44.13±10.93) % and (25.93±8.37) %. When HepG2 cells were cultured for 72 h, the inhibition rate of HepG2 cells diluted 5 and 10 times was (59.47±6.90) % and (41.02±4.27) %. The flow cytometry showed that after incubated with the leaf of fast-growing eucylyptus extract for diluted 30 times, the early of apoptosis were 30.03%. The ratios of apoptotic increased with concentration diluted times decreased, and it was in a time-dependent manner. Moreover, the ratios of apoptotic increased with the time prolonged of the leaf of fast-growing eucylyptus extract, and it was in a dose-dependent manner. Conclusion: The leaf of fast-growing eucylyptus extract can inhibit proliferation and induced apoptosis on HepG2 Cells in a time-and dose-dependent manner. |
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