| 谢孟凌,王澎弘,王顺德,杨 群,李美材.肾虚育精方对肾精亏虚小鼠模型生殖激素及氧化应激水平的影响[J].,2018,(13):2457-2460 |
| 肾虚育精方对肾精亏虚小鼠模型生殖激素及氧化应激水平的影响 |
| Effects of Shenxu Yujing Recipe on Reproductive Hormone and Oxidative Stress in Mice Model of Kidney Deficiency |
| 投稿时间：2017-10-13 修订日期：2017-10-30 |
| DOI：10.13241/j.cnki.pmb.2018.13.011 |
| 中文关键词: 肾虚育精方 肾精亏虚 小鼠 生殖激素 氧化应激 |
| 英文关键词: Shenxu Yujing recipe Kidney deficiency Mice Reproductive hormone Oxidative stress |
| 基金项目:2017年重庆市中医药科技项目（ZY201703066） |
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| 中文摘要: |
| 摘要 目的：研究肾虚育精方对肾精亏虚小鼠模型生殖激素及氧化应激水平的影响。方法：选择6周龄雄性健康级SD小鼠60只纳入本次研究。根据随机数字法将小鼠分成3组，分别为观察组、模型组及对照组，每组各20只。观察组及模型组的小鼠通过"房劳和惊恐复合伤肾法"建立肾精亏虚模型，对照组小鼠不建模。建模完成后观察组小鼠每日给予肾虚育精方的浓缩液干预，模型组及对照组则予以等量的生理盐水灌胃。干预21d后对比各组小鼠睾丸组织有关指标，包括Johnsen 10级积分、生精小管的直径及生精上皮厚度，小鼠生殖激素水平，包括血清卵泡刺激素（FSH）及睾酮（TEST），氧化应激指标水平，包括丙二醛（MDA）、过氧化物歧化酶（SOD）及总抗氧化能力（T-AOC）。结果：对照组与观察组的Johnsen 10级积分高于模型组，生精小管的直径及生精上皮厚度大于模型组，差异均有统计学意义（P＜0.05）；观察组的生精上皮厚度明显大于对照组，差异有统计学意义（P＜0.05）。对照组与观察组小鼠的FSH及TEST水平均明显高于模型组，差异均有统计学意义（均P＜0.05）。对照组与观察组的MDA和SOD水平明显低于模型组，T-AOC水平明显高于模型组，差异均有统计学意义（P＜0.05）。结论：肾虚育精方能够提升肾精亏虚小鼠模型的生殖激素水平，并降低氧化应激水平，可将肾虚育精方应用于临床治疗。 |
| 英文摘要: |
| ABSTRACT Objective: To study the effects of Shenxu Yujing recipe on reproductive hormone and oxidative stress in mice model of kidney deficiency. Methods: 60 male healthy SD mice with 6 weeks were included in the study. According to the random number method, the mice were divided into 3 groups, which were the observation group, the model group and the control group, with 20 mice in each group. The mice in the observation group and the model group were established with the kidney deficiency model by the combination of sexual strain and terrified, the mice in the control group were not modeled. After the modeling, the mice in the observation group were given the concentrated solution of the Shenxu Yujing recipe every day, the model group and the control group were given the same amount of normal saline.After the intervention of 21d, the relative indexes of testis tissue in each group were compared, which were included Johnsen 10 stage integral, the diameter of the seminiferous tubules, thickness of seminiferous epithelium, and reproductive hormone levels in mice, including the serum follicle stimulating hormone (FSH) and testosterone (TEST), oxidative stress index, including malondialdehyde (MDA), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC). Results: The Johnsen 10 stage integral in the control group and the observation group was higher than that in the model group, the diameter of seminiferous tubules and the thickness of seminiferous epithelium were higher than those of the model group, the differences were statistically significant (P<0.05). The levels of FSH and TEST in the control group and the observation group were significantly higher than those in the model group, the differences were statistically significant (P<0.05). The levels of MDA and SOD in the control group and the observation group were significantly lower than those in the model group, and the level of T-AOC was significantly higher than that in the model group, the differences were statistically significant (P<0.05). Conclusion: The Shenxu Yujing recipe can raise the level of reproductive hormone in mice model of kidney essence deficiency, and reduce the oxidative stress level, which can be used in clinical treatment. |
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