| 张 莉,张孟玲,郭菲菲,孙向荣,高胜利,徐 珞.侧脑室注射Orexins对大鼠摄食的影响及机制[J].,2017,17(1):28-31 |
| 侧脑室注射Orexins对大鼠摄食的影响及机制 |
| Effects of Orexins on the Food Intake of Rats and Its Mechanism |
| 投稿时间：2016-05-06 修订日期：2016-05-27 |
| DOI：10.13241/j.cnki.pmb.2017.01.007 |
| 中文关键词: Orexins NPY MCH 甘丙肽 摄食 |
| 英文关键词: Orexins NPY MCH Galanin Food intake |
| 基金项目:国家自然科学基金项目（81470815，81270460，81300281，81500414）；山东省优秀中青年科学家科研奖励基金项目（BS2014YY009）；青岛市科技局基金项目（13-1-4-170-jch；14-2-3-3-nsh） |
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| 中文摘要: |
| 摘要 目的：探讨侧脑室注射orexins(食欲素)、NPY(神经肽Y)、MCH(黑色素聚集激素)和甘丙肽对大鼠摄食的影响及其机制。方法：将成年雄性Wistar大鼠随机分为对照组、侧脑室注射组和室旁核(PVN)注射组。通过套管将orexin-A、orexin-B、NPY、MCH和甘丙肽分别注射至侧脑室和PVN内，随后测量大鼠食物摄入量，并检测PVN、弓状核(ARC)和VMH内c-fos的表达。结果：与对照组比较，侧脑室注射NPY、MCH和orexin-B 2 h后，大鼠摄食量显著增多(P＜0.05)。相较于orexin-B和MCH，NPY对摄食的影响更显著(P＜0.05)。与NS对照组比较，侧脑室注射甘丙肽和orexin-A 1 h后，大鼠摄食量显著增多(P＜0.05)。与NS对照组比较，侧脑室注射orexin-A可显著增加c-fos在PVN和ARC中的表达，在VMH中效应较弱(P＜0.05)。与NS对照组比较，PVN注射NPY能显著增加大鼠2 h摄食量(P＜0.05)，PVN注射orexin-A能显著增加大鼠2 h和4 h摄食量(P＜0.05)。结论：orexins与可促进大鼠摄食，此效应可能通过下丘脑参与摄食调控中枢PVN和ARC而实现的。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the role of the orexins, NPY, melanin-concentrating hormone (MCH) and galanin in food in- take in rats and their mechanism. Methods: Male wistar rats were randomly divided into sham group, lateral ventricle (ICV) group and PVN group. Orexin-A, orexin-B, NPY, MCH and galanin were respectively injected into ICV and PVN through the cannula, then mea- sured the food intake of rats to compared their effect. Also the expression of c-fos was detected in PVN, ARC and VMH. Results: Com- pared with sham group, the 2 h food intake of rats following injected NPY, MCH and orexin-B into ICV had significantly increased (P＜0.05). The effect of NPY in food intake was more obviously than orexin-B and MCH (P＜0.05). Compared with sham group, the 1 h food intake of rats following inject galanin and orexin-A into ICV had significantly increased (P＜0.05). C-fos was expressed in PVN and ARC markedly (P＜0.05). Compared with sham group, the expression of c-fos was greatly inhanced after orexin-A administered into ICV, weakly in VMH (P＜0.05). Compared with sham group, the 2 h food intake of rats following injected NPY into PVN had signifi- cantly increased (P＜0.05), orexin-A markedly increased the food intake of rats at 2 h and 4 h (P＜0.05). Conclusion: Orexin-A can stimulated food intake of rats, and it may related to its physiology role in appetite regulation. |
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