| 丁冬 郝铁成 曹军 牛建国 何仲义.嗅鞘细胞的不同纯化方法及结果[J].,2014,14(19):3766-3769 |
| 嗅鞘细胞的不同纯化方法及结果 |
| The Results of Olfactory Ensheathing CellsfromDifferent Purification Methods |
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| DOI： |
| 中文关键词: 嗅鞘细胞 细胞培养 细胞纯化 纯化方法 |
| 英文关键词: Olfactory ensheathing cells Cultivation Purification |
| 基金项目:2011 年银川市科技发展项目（银财发[2011]265 号）；2012年宁夏自治区科技支撑项目（宁科计字[2012]17 号）；
宁夏医科大学博士建设开放课题（KF2010-18） |
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| 中文摘要: |
| 培养的嗅鞘细胞的最终纯度受到多种因素的影响，如嗅鞘细胞的取材来源、分离方法等等；对培养的嗅鞘细胞进行纯化可
获得高纯度的嗅鞘细胞。纯化嗅鞘细胞的方法有许多种，主要有单纯差速贴壁法、免疫吸附法、化学药物抑制法、无血清饥饿法
等，现在的实验研究更趋向于以上2-3 种方法联合应用对嗅鞘细胞进行纯化，这些联合纯化方案主要是在采用单纯差速贴壁方法
的基础上再次运用其他一种或几种方法进行嗅鞘细胞的纯化。就获取的嗅鞘细胞的最终纯度而言，许多方法取得了可观的效果。
但不同的纯化方法各有利弊，除了价格不同外，不同的纯化方法对嗅鞘细胞的生物活性造成不同程度的影响。因此在选择纯化方
法时，应综合考虑各方面因素，根据研究目的和实际需要选择合理的方案进行纯化。本文通过查阅各数据库中与嗅鞘细胞的分离
培养及纯化有关的文献和其他相关书籍，来探讨纯化嗅鞘细胞的不同方法以及这些纯化方法对嗅鞘细胞最终纯度的影响。 |
| 英文摘要: |
| The final density of olfactory ensheathing cells can be determined by several factors, such as the source and dissociate
methods of cells; high density olfactory ensheathing cells can be got by purificating them. There are many kinds of purification methods
for olfactory ensheathing cells, including Different-Time adherence method, immunosorbent method, chemical drugs method, etc, The
recent researches rend to combine 2 or 3 methods above to purificate the olfactory ensheathing cells, these purification plans combine 1
or several other methods on the basis of Different-Time adherence method.In terms of the final purity of olfactory ensheathing cells, many
methods have got considerable effects. But different purification methods have advantages and disadvantages, in addition to the different
price, different purification methods have different influence to biological activities of olfactory ensheathing cells. So when choosing
purification methods, various aspects should be considered, according to the research purpose and the actual need to select the reasonable
plan for purification. This article researches some literatures and relative books about culturing and purificating the olfactory ensheathing
cells from some database to explore the different methods of purificating the olfactory ensheathing cells and the effects to the olfactory
ensheathing cells, final density of these methods. |
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