| 郭枫 钟鸣 杨乃林 卞正乾 赵刚.肿瘤干细胞标记物CD133与miR-429 在大肠癌组织中的
表达及其相关性研究[J].,2014,14(19):3684-3686 |
| 肿瘤干细胞标记物CD133与miR-429 在大肠癌组织中的
表达及其相关性研究 |
| Corelation and Expressions of CD133 and miR-429 in the Tissues ofColorectal Carcinoma |
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| DOI： |
| 中文关键词: 肿瘤干细胞 CD133 miR-429 |
| 英文关键词: Tumor stemcell CD133 MiR-429 |
| 基金项目: |
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| 中文摘要: |
| 目的：检测CD133不同亚群大肠癌细胞HT-29 的miR-429 表达情况，探讨miR-429 及CD133的表达与肿瘤的发生发展之间
的关系。方法：采用荧光活化细胞分选法（FACS）分选出CD133不同亚群细胞，实时荧光定量PCR分别检测两组细胞miR-429 的
表达，合成miR-429 寡核苷酸和阴性对照miRNA并分别转染CD133
+和CD133
-两个亚群细胞。再将细胞种植于非肥胖糖尿病/ 严
重联合免疫缺陷(NOD/SCID) 小鼠体内构建移植瘤模型，不同时间测量肿瘤体积和重量，RT-PCR及蛋白质印迹检测CD133
+和
CD133
-两组肿瘤CD133mRNA和蛋白质表达。结果：血清检出CD133
+细胞为67.9%，miR-429 的表达量是CD133
+细胞的（1.83± 0.91）
倍（P<0.05），CD133
+比例与miR-429 表达呈负相关（r=0.591，P<0.05）；miR-429+/CD133
+组的移植瘤体积及重量与对照组比较有统
计学差异（P<0.05），且miR-429+/CD133
+组成瘤时间较对照组晚约2 周，但miR-429+/CD133
+组的移植瘤CD133表达量低，与阴性对
照组比较无明显差异（P>0.05）。结论：miR-429 可能作为CD133的负性调控因子，具有抑制肿瘤生长的作用，但miR-429 与CD133
在肿瘤发生、发展过程中的作用机制有待进一步研究阐明。 |
| 英文摘要: |
| Objective:To detect the expressions of miR-429 in different CD133 subgroups on the HT-29 cells in the colorectal
cancer tissues of which to explore the corelation between the occurrence and progression of tumors.Methods:The different CD133
subgroup cells were sorted out by fluorescence activated cell sorting (FACS); the expression of miR-429 was detected by Real-time PCR;
synthetic oligonucleotides of miR-429 and the negative control of microRNA were transfected in CD133
+ and CD133
- subgroups; the
non-obese diabetic/severe combined immunodeficiency mice (NOD/SCID) were planted the transfected cells to construct the transplanted
tumor model; the tumor volume and weight were measured in different time; the expression of CD133 mRNA and protein in these two
groups were detected by RT-PCR and western blotting.Results:The proportion of CD133
+cells was 67.9%; the expression of miR-429 in
CD133
- cell was (1.83+0.91) times of CD133
+'s (P<0.05); the corelation of CD133
+ 's ratio and the expression of miR-429 was negative(r=0.
591, P<0.05); there were statistically significant differences in the volume and weight of transplanted tumors in miR-429+/CD133
+ between
the two groups(P<0.05); the tumorigenicity of miR-429+/CD133
+ group was two weeks later than that of the control group, while the CD133
expression was lower with no significant difference than the negative control group(P>0.05).Conclusion:It is suggested that the miR-429
might be a negative regulatory factor of CD133 which could inhibite the growth of tumor. However, the mechanism of miR-429 and CD133
in process of tumor occurrence and development needs to be further studied and clarified. |
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