| 程玉1 张宇1 胡志强2 李国忠1 项莹3 胡恩喜1
王超4 关峰2 陶宇1郑宏山1.Ras蛋白在地塞米松体外诱导大鼠胚胎垂体生长激素细胞
分化过程中的作用*[J].,2014,14(17):3228-3231 |
| Ras蛋白在地塞米松体外诱导大鼠胚胎垂体生长激素细胞
分化过程中的作用* |
| The effect of Ras on Dexamethasone-induced Rat Embryonic PituitarySomatotroph Cell Differentiation in vitro* |
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| DOI： |
| 中文关键词: 地塞米松 垂体 生长激素细胞 分化 Ras蛋白 |
| 英文关键词: Dexamthasone Pituitary GHcell Differentiation Ras protein |
| 基金项目:黑龙江省教育厅基金项目（11513053） |
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| 中文摘要: |
| 摘要目的：探究Ras蛋白在地塞米松体外诱导大鼠胚胎垂体生长激素细胞分化过程中的作用。方法：本课题利用大鼠胚胎垂体细胞
的无血清原代细胞培养技术，在地塞米松诱导生长激素细胞分化的过程中，加入蛋白Ras的抑制剂Manumycin，利用免疫荧光、
western-blot、放射免疫分析和MTT等技术对Ras蛋白在糖皮质激素体外诱导生长激素细胞分化中的作用进行研究。结果：地塞米
松能够显著提高生长激素阳性细胞百分比和生长激素的含量(P<0.01)。加入不同浓度的Manumycin后，地塞米松诱导的生长激素阳
性细胞百分比显著降低(P<0.01)，生长激素的含量亦出现降低（P<0.05）。结论：Ras蛋白在地塞米松诱导垂体生长激素细胞分化过程
中发挥重要作用。 |
| 英文摘要: |
| ABSTRACT Objective:The function of Ras is to be explored in dexamethasone-induced differentiation of rat embryonic pituitary
somatotroph cells in ivtro. Methodsd:The pituitary was harvested for fetal pituitary cell suspension. In the experiment Ras inhibitor
Manumycin of different concentrations were added to the suspension for pretreatment and cells were cultured with a medium containing
Dex. And also immunofluorescence, Western-blot, radioimmunoassay and MTT Technology were used in this experiment. Rethods:Dex
treatment increased the percentage of GH-positive cells and GH content (P<0.01). After pretreatment with Manumycin, the percentage of
Dex-induced GH-positive cells decreased significantly (P<0.01), and the GH content also reduced (P<0.05). Conclusion:Ras protein plays
an important role in the dexamethasone-induced growth hormone cell differentiation. |
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