| 方茅 龙捷 王红艳 谢晓斌 Thoidingjam Bidyarani Chanu 陈启宪.DJ-1 基因siRNA对三阴性乳腺癌细胞侵袭
和迁移影响的研究[J].,2014,14(7):1239-1242 |
| DJ-1 基因siRNA对三阴性乳腺癌细胞侵袭
和迁移影响的研究 |
| Influence of RNA Interference Induced Silencing of DJ-1 Geneon Migration and Invasion Potentialin Triple-Negative Breast Cancer Cells |
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| DOI： |
| 中文关键词: DJ-1 三阴性乳腺癌 迁移 侵袭 RNA干扰技术 |
| 英文关键词: DJ-1 Triple-negative breast cancer Migration Invasion RNA interference |
| 基金项目:广东省自然科学基金项目（S2012040007232）；广州市属高校科研计划项目（2012C202）
广州医学院科学研究项目（L110503）；2012-2013 年度广州医学院大学生课外科技活动项目（2012A001；2012A006） |
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| 中文摘要: |
| 目的：探讨DJ-1 基因siRNA 对三阴性乳腺癌细胞体外侵袭和迁移能力的影响。方法：设计DJ-1 基因的小分子干扰RNA
(siRNA)片段，脂质体介导转染入三阴性乳腺癌细胞株MAD-MB-23l，转染分3 个组：A 组（空白对照control 组）、B 组(转染非特
异性对照Scramble 组)、C 组(转染si DJ-1 组)。应用Western blotting免疫印迹法检测转染前后DJ-1 表达水平；运用细胞迁移和侵
袭实验检测细胞迁移和侵袭能力的变化。结果：C组DJ-1 蛋白的表达强度弱于A 组和B 组(t=9.831，P<0.05)，而A组与B 组比
较，DJ-1蛋白表达水平则无明显差异(t=1.629，P>0.05)。细胞迁移实验中，A 组细胞为(218.37± 12.75)；B组的细胞为(214.46±
11.38) ；C 组的细胞为(129.65± 8.59)，C 组细胞明显少于A 组和B 组(t=10.927，9.984，P<0.05)，而A 组与B 组之间，差异无统计
学意义(t=0.512，P>0.05)。细胞侵袭实验中，A 组细胞为(127.28± 12.65)；B 组的细胞为(123.06± 13.08) ；C 组的细胞为(52.85±
9.58)，C 组穿过人工基底膜的细胞明显少于A 组和B 组(t=7.927，8.643，P<0.05)，而A 组与B 组之间，差异无统计学意义(t=0.627，
P>0.05)。结论：DJ-1 基因siRNA 可抑制三阴性乳腺癌细胞侵袭和迁移。 |
| 英文摘要: |
| Objective:To investigute the effect of RNA interference induced silencing of DJ-1 gene on migration and invasion
potential in triple-negative breast cancer cells.Methods: Human breast cancer cells MAD-MB-23l were divided into control group(A
group)，Scramble group(B group) and si DJ-1 group(C group). siRNA sequence targeting DJ-1 gene were designed and synthesized. The
siRNA were transfected into triple-negative breast cancer cell by lipofectamine TM 2000 mediation. The alteration of DJ-1 protein
expression was detected by western blotting, Invasion potential were evaluated by transwell invasion assay, Cell migrating ability was
detected by transwell migration assay.Results:Transfection of DJ-1 siRNA significantly knocked down the DJ-1 protein expression, The
migration and invasion of breast cancer cells declined after transfection with siRNA DJ-1, DJ-1 protein expression of C group was lower
than that of A group and B group (t=9.831, P<0.05). There was no difference between A group and B group (t=1.629, P>0.05). In cell
migration assay, the number of cells in C group (129.65± 8.59) was less than that in A group (218.37± 12.75) and B group (214.46±
11.38) (t=10.927, 9.984, P<0.05). There was no difference betweenA group and B group (t=0.512, P>0.05). In cell migration assay, the
number of cells in C group(129.65± 8.59) was less than that in A group(218.37± 12.75) and B group(214.46± 11.38) (t=10.927, 9.984,
P<0.05). There was no difference between A group and B group (t=0.512, P>0.05). In invasion potential test, the number of cells in C
group(52.85± 9.58) was less than that in A group (127.28± 12.65) and B group (123.06± 13.08) (t=7.927, 8.643, P<0.05). There were
no difference between A group and B group (t=0.627, P>0.05).Conclusion:The siRNA targeting DJ-1 gene can restrain the migration
and invasion of triple-negative breast cancer cell. |
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