| 徐义喜
1,2
栾天竹
1△
史平炜
2
周立君
1
徐卿璐
1
刘苏来.匹伐他汀对体外培养人脐静脉血内皮祖细胞数量及功能的影响[J].,2014,14(6):1053-1056 |
| 匹伐他汀对体外培养人脐静脉血内皮祖细胞数量及功能的影响 |
| Effects of Pitavastatin on Number and Activity of Endothelial ProgenitorCells from Umbilical Vein Blood |
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| DOI： |
| 中文关键词: 内皮祖细胞 匹伐他汀 增殖 迁移 粘附 |
| 英文关键词: Endothelial progenitor cells Pitavastatin Proliferation Migratory Adhesion |
| 基金项目: |
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| 中文摘要: |
| 摘要 目的： 通过体外培养人脐静脉血内皮祖细胞 （endothelial progenitor cells， EPCs ）， 观察他汀类新药（ 匹伐他汀）对 EPCs 数量及
增殖、 迁移和粘附功能的影响。方法： 采用密度梯度离心法分离培养人脐静脉血单个核细胞， 将其接种在包被有人纤维连接蛋白
培养板上， 培养 7d 后， 收集贴壁细胞， 加入不同浓度匹伐他汀(分别为 0.001 μmol/L 、 0.01 μmol/L、 0.1 μmol/L、 1.0 μmol/L)培养 24
h，用免疫荧光法观察 EPCs 吸收 FITC-UEA-I 和 Dil-acLDL 情况对 EPCs 进行鉴定， 然后分别采用 MTT 比色法、 改良的 Boyden
小室、 粘附能力测定实验对各实验组测定， 来观察匹伐他汀对 EPCs 数量及增殖、 迁移和粘附功能影响。结果： 匹伐他汀组与对照
组相比， 匹伐他汀显著提高了体外培养 EPCs 的数量及增殖、 迁移与粘附能力(P<0.05)。匹伐他汀浓度在 0.1 μmol/L 时对 EPCs 影
响达到最大。随着药物浓度的继续增大， EPCs 的上述功能反呈下降趋势， 但 1.0 滋mol/L 组仍高于对照组。结论： 匹伐他汀能增加
体外培养 EPCs 的数量及增殖、 迁移和粘附能力， 可作为 EPCs 培养的一种改良方法， 为其更好的应用于临床具有重要的意义。 |
| 英文摘要: |
| ABSTRACT Objective：To investigate influences of Pitavastatin on adhesion, spreading, migration of endothelial progenitor cells
(EPCs). Methods:Total mononuclear cells (MNCs) were isolated from peripheral blood by Ficoll density gradient centrifugation, and
then the cells were plated on fibronectin-Coated culture dishes. After culture for7 days, attached cells were stimulated with Pitavastatin (to
make a series of final concentrations: 0.001 μmol/L, 0.01 μmol/L, 0.1 μmol/L, 1.0 μmol/L) for 24 h. EPCs were characterized as adherent cells double positive for DiLDL2 uptake and lectin binding by direct fluorescent staining. EPCs proliferation, migration were assayed
with MTT assay and modified Boyden chamber assay, respectively. EPCs adhesion assay was performed by replating those on fibronectin
2 coated dishes, and then adherent cells were counted.Results: Incubation of isolated human MNCs with Pitavastatin dose dependently
increased the number of EPCs, maximum at 0.1 μmol/L, (P<0.05). In addition, Pitavastatin also promotes EPCs proliferation, migratory
and adhesive capacity ina concentration dependent manner. When the concentration is higher than 1.0 滋mol/L, the function of EPCs will
be inhibited.Conclusion: The results of the present study defined that Pitavastatin can promote EPCs proliferative, migratory and adhesive capacity, and can be a new way for culture EPCs. All of these can provide more advise for clinical. |
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