| 刘婷婷 张玥 王丹丹 张彤帅 张瑶
王菁华 孙博 尚小煜 李呼伦 王广友.CD8+CD122+T细胞在脑缺血过程中作用的研究[J].,2014,14(1):77-81 |
| CD8+CD122+T细胞在脑缺血过程中作用的研究 |
| Effect of CD8+CD122+T Cells on Cerebral Ischemia Injury in Mouse |
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| DOI： |
| 中文关键词: 脑缺血 CD8+CD122+T细胞 氧糖剥夺 星形胶质细胞 |
| 英文关键词: Cerebral ischemia CD8+CD122+T cell Oxygen-glucose deprivation Astrocytes |
| 基金项目:国家自然科学基金项目（81070943）；黑龙江省教育厅面上项目（11541234） |
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| 中文摘要: |
| 目的：探讨CD8+CD122+T细胞在脑缺血过程中的作用及其机制。方法：线栓法建立小鼠大脑中动脉栓塞模型（MCAO）；激光
共聚焦显微镜检测小鼠脑缺血组织中CD8+CD122+T细胞浸润情况；流式细胞仪检测脑缺血组织中CD8+CD122+T细胞/CD3+T 细
胞的比例及脾和胸腺中CD8+CD122+T细胞数量变化；RT-PCR 方法检测CD8+CD122+T细胞对氧糖剥夺（Oxygen-glucose
deprivation, OGD）条件下星形胶质细胞表达TNF-α，IL-1β，IFN-γ的影响。结果：各时间点脑缺血组织中均有CD8+CD122+T细胞浸
润，且随脑缺血时间延长，缺血侧脑组织中CD8+CD122+T细胞/CD3+T 细胞比例逐渐增加，5 d和7 d组差异显著，与非缺血侧相
比，P5d<0.05，P7d<0.05；MCAO 小鼠脾及胸腺中CD8+CD122+T细胞呈现先增高后降低的趋势。星形胶质细胞经OGD处理后，与对
照组相比，IFN-γ、TNF-α、IL-1β表达显著增高，PIFN- γ<0.01、PTNF- α<0.001、PIL-1β<0.01；CD122-blocked 组与CD8+ 组相比，IFN-γ、
TNF-α、IL-1β表达明显增高，PIFN-γ<0.05、PTNF-α<0.05、PIL-1β<0.01；CD8+组与HBSS 组相比，IFN-γ表达降低，P<0.05；IL-1β表达有降
低的趋势。结论：CD8+CD122+T细胞在脑缺血过程中发挥保护性作用，其保护作用通过CD122 抑制星形胶质细胞TNF-α，IL-1β，
IFN-γ炎症因子表达实现的。 |
| 英文摘要: |
| Objective: To investigate the effects of the CD8+CD122+T cells on the experimental brain ischemia.Methods:The
animal model of the brain ischemia was set up via the brain middle-artery occlusion by inserting nylon. Confocal microscopy analysis
was applied to detect the CD8+CD122+T cells in the ischemic brain. Quantitative flow cytometric analysis was used to measure the
inflammatory cells in ischemic brain, spleen and thymus. The relative amount of TNF-γ, IL-1βand IFN-αmRNA in astrocytes after
Oxygen-glucose-deprivation treatment was detected by RT-PCR.Results:There were CD8+CD122+T cells infiltrated in the ischemic
brain at all the time points compared with the contralateral. There was significant difference at 5 d and 7 d (P5d<0.05, P7d<0.05).
CD8+CD122+T cells displayed the amount variation in the spleen and thymus by initial increase then decrease. After the treatment of
OGD, there was a significant increasing expression of IFN-γ, TNF-α, IL-1βmRNA in astrocytes compared with that in the control group
(PIFN-γ<0.01, PTNF-α<0.001, PIL-1β<0.01). The inhibition of IFN-γ, TNF-α, IL-1βmRNA by CD8+T cells administration was reversed when
CD122 was blocked (PIFN-γ<0.05, PTNF-α<0.05、PIL-1β<0.01).Conclusion:CD8+CD122+T cells take part in the process of brain ischemia and
they display a significant neuroprotective effects by inhibiting the expression of TNF-α，IL-1βand IFN-γmRNA in astrocytes. |
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