| 孟乐乐1 洪晓芸1 张奇昕1 吴造展1 张丽君1 廖美玲1 张向荣2 袁伟恩1△.干扰素α 缓释微球的制备及体外释放研究*[J].,2012,12(27):5201-5203 |
| 干扰素α 缓释微球的制备及体外释放研究* |
| The Preparation and Research of Vitro-release of Interferon-loadedMicrospheres* |
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| DOI： |
| 中文关键词: 干扰素α 长效缓释 体外释放 |
| 英文关键词: Interferon α Sustained-release In vitro release |
| 基金项目:国家重大专项—" 重大新药创制" 科技重大专项—创新药物研究开发技术平台（2009ZX09310-007）；
国家自然科学基金（81173001）；上海科委纳米专项（No11nm0503300 和No.1052nm03900） |
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| 中文摘要: |
| 目的：开发一种有效地长效缓释干扰素α 微球制剂。方法：利用S/O/W乳剂- 挥发法制备了包裹干扰素α 多糖颗粒的PLAG
微球，对其外观形态进行了考察，并用ELISA 方法考察了微球体外释放效果。结果：制备的干扰素α 微球圆整光滑，粒径均匀；经
24 天体外释放，累计释放率达到80%以上。结论：通过包封包裹干扰素α 的多糖颗粒进PLGA 微球，有效地保护了干扰素α 在微
球中的活性，实现了长效缓释，是一种可行的缓释方案。 |
| 英文摘要: |
| Objective: To develop an effective sustained-release preparation of interferon α. Methods: Interferon α (IFN α)-loaded
dextran nanoparticles were prepared by the method of freezing-induced phrase separation, then the IFNα-loaded dextran nanoparticles
were microencapsulated in PLGA microspheres by the method of solid-in-oil-in-water (S/O/W) emulsion-evaporation technique. The
microsphere samples were analyzed by using SEM. In vitro release profiles of the samples were detected by Elisa Kit. Results: The
IFN-loaded microspheres had spherical shape and smooth surface. They possessed a normal size distribution. The accumulated release in
vitro was over 80% after 24 days. Conclusion: The preparation of PLGA microspheres of IFN α through the encapsulation of IFN
α-loaded Dextran nanoparticles protected the bioactivity of the IFN α in the microspheres effectively and got an ideal sustained release
profile. This method is an effective technique for IFN α sustained release. |
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