| 张林1 刘伏友2△ 彭佑铭2 段绍斌2 刘虹2 李瑛2.人白蛋白对HK-2 细胞凋亡的作用[J].,2012,12(26):5041-5044 |
| 人白蛋白对HK-2 细胞凋亡的作用 |
| Effects of Human Albumin on the Apoptosis of HK-2 Cells |
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| DOI： |
| 中文关键词: 人白蛋白 HK-2细胞 凋亡 |
| 英文关键词: Human albumin Renal tubular epithelial cells Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨人白蛋白对体外培养的HK-2 细胞凋亡的作用。方法：本实验研究对象为HK-2 细胞株，将培养的HK-2 细胞与
20g/L的人白蛋白共同孵育0、4、6、8小时后，用Hoechst33258染色检测细胞凋亡。不同浓度(0g/L、5 g/L、10 g/L、20g/L和30g/L)的
人白蛋白与体外培养的HK-2 分别共同孵育0、4、6、8 小时后，流式细胞仪检测细胞凋亡。结果：Hoechst33258 染色结果显示：培
养基对照组未见明显细胞凋亡；20g/L白蛋白与HK-2细胞共同孵育4、6、8 小时，与对照组比较，均可见HK-2 细胞荧光强度增
加，有着典型凋亡形态的细胞增多，且随着人白蛋白与HK-2细胞作用时间的延长，细胞凋亡的程度和数目也增多。流式细胞仪检
测结果显示：与对照组比较，HK-2细胞的凋亡率随着人白蛋白与HK-2细胞作用的浓度和时间增加而显著性增高，细胞凋亡率在
8h 组为(9.15±0.15%)，在30g/L组为（9.35±0.46%)，均为最高。结论：人白蛋白以时间和剂量依赖方式诱导肾小管细胞凋亡，以
30g/L作用浓度和8小时作用时间的人白蛋白诱导HK-2细胞凋亡的作用最显著。 |
| 英文摘要: |
| Objective: To investigate the effects of human albumin on the apoptosis of renal tubular epithelial HK-2 cells in
vitro．Methods: HK-2 cells were incubated with various concentrations(0g/L, 5g/L, 10 g/L, 20 g/L, 30g/L) of human albumin for 0h, 4h,
6h and 8h. The apoptosis of HK-2 cells were evaluated by Hoechst33258 fluorescence stained cytospins and flow cytometry. Results: No
significant apoptosis was observed in the control group treated by normal culture medium, but the fluorescence intensity and number of
typical apoptotic cells increased in the cells treated with 20g/L human albumin for 4, 6 and 8 hours, and they increased gradually when
the time extended. The flow cytometry results showed that the rates of apoptosis in HK-2 cells rose significantly along with increasing
concentrations of human albumin and prolonging of incubation time, which was the highest at concentration of 30g/L and for 8h
(respectively 9.15±0.15％ and 9.35±0.46％). Conclusions: Human albumin induced the apoptosis of HK-2 cells in a dose and
time-dependent manner. It got the most significant effect when it lasted for 8h at concentration of 30g/L. |
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