| 周晓曦李钦璐黄闪姜利军周剑峰曹阳△.静脉输注新型溶瘤腺病毒在体内复制活性与抑瘤的关系*[J].,2012,12(19):3624-3627 |
| 静脉输注新型溶瘤腺病毒在体内复制活性与抑瘤的关系* |
| The Relationship of Replication and Inhibiting Tumor of New OncolyticAdenovirus by Intravenous Infusion in Vivo |
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| DOI： |
| 中文关键词: 溶瘤腺病毒 静脉输注 分布 |
| 英文关键词: Oncolytic adenovirus Intravenous infusion Distribution |
| 基金项目:国家自然科学基金青年基金(81001049) |
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| 中文摘要: |
| 目的：观察新型溶瘤腺病毒M1 在体内代谢的时间及对肿瘤生长的抑制作用。方法：建立人胃癌裸鼠原位移植瘤动物模型，
在指定时间点处死动物，观察瘤体大小，分离原发瘤及转移瘤进行病毒滴度测定，采用免疫组化及原位杂交来检测病毒的分布和
活性复制。结果：在人胃癌裸鼠原位模型上，M1 经静脉输注后不仅分布于胃原发瘤，而且存在于转移灶，但用M1 静脉内给药可
以减缓肿瘤生长速度。结论：静脉内输注溶瘤腺病毒可以选择性的作用于肿瘤及转移灶，并发挥溶瘤和靶向灭活plk1 的双重效
应。 |
| 英文摘要: |
| Objective: To investigate the metabolism time of new oncolytic adenovirus M1 distributing in body and effects of
inhibiting tumor. Methods: The stomach transplanting tumour changes the model of nude mouse was constructed, The mouse was killed
in time, and the size of tumor was detected. Virus titer of primary tumor and metastasis and distribution of virus were assayed with
immunohistochemistry and In situ hybridization. Results: In human cancer of the stomach transplanting tumour changes the model of
nude mouse, M1 distributes over primary tumor and metastasis by intravenous infusion, but M1 can inhibit growth of tumor by
intravenous infusion. Conclusion: Oncolytic adenovirus can distribute over tumor and metastasis, kill the tumor and plk1. |
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