| 闫丽娟2 康燕2 李宝华2.循环DNA 和HBV-DNA 载量与原发性肝癌的相关性探讨[J].,2011,11(19):3713-3715 |
| 循环DNA 和HBV-DNA 载量与原发性肝癌的相关性探讨 |
| Correlation of Circulating DNA and HBV-DNA Load and PrimaryLiver Cancer |
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| DOI： |
| 中文关键词: 原发性肝癌 血循环DNA HBV-DNA |
| 英文关键词: PHC Blood circulating DNA HBV-DNA |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨血浆DNA 和HBV-DNA 载量在病毒性肝炎肝硬化后原发性肝癌(PHC)的诊断价值。方法：用RT-PCR 方法随访
检测HBsAg 阳性且经彩色B 超诊断为肝硬化的患者，每6 个月检测一次患者的血液循环DNA 和HBV-DNA 载量，直到经临床
症状、彩色B 超、CT 诊断为原发性肝癌为止。结果：在PHC 中，循环DNA 61.3±31.9ng/ml,健康对照组20.3±9.9ng/ml，两组差异
显著（P＜0.01）；而HBV-DNA 载量，在PHC 确诊时多数处于较低水平，78%≤105copies/ml，与PHC 确诊前有明显差异（P＜
0.01）。PHC 未发生淋巴结转移者循环DNA 51.2±17.9ng/ml，淋巴结转移者循环DNA 73.9±23.7ng/ml，两者存在差异（P＜0.05）；
而HBV-DNA 载量有无淋巴结转移无明显差异。结论：联合检测血液循环DNA 和HBV-DNA 载量对PHC 的预测、预后价值明
显，且方法简单，易于开展。 |
| 英文摘要: |
| Objective: To explore the diagnosis value of plasma DNA and HBV-DNA load for PHC after viral hepatitis cirrhosis.
Methods: Follow-up with RT -PCR to test the hepatitis cirrhosis patients by Color B ultrasonic diagnosis who HBsAg is positive. To test
the patients with blood circulating DNA and HBV-DNA load every six months，until diagnosis of PHC by the clinical symptoms, color B
ultrasonic and CT. Results: Blood circulating DNA is 61.3±31.9ng/ml in PHC, health control group is 20.3±9.9ng/ml, two groups have
significant difference （P＜0.01）, and HBV-DNA load is low level，78%≤105copies/ml，and have obvious difference with PHC who has
not diagnosed （P＜0.01）. The blood circulating DNA of PHC who has not occurred lymph node metastasis is 51.2±17.9ng/ml, who has
occurred lymph node metastasis is 73.9±23.7ng/ml, differences exist between them （P＜0.05）; But HBV-DNA load has no obvious
difference between patients with or without lymph node metastasis. Conclusion: United detecting of the blood circulating DNA and
HBV-DNA load for the forecasting and prognosis value of the PHC is obvious, and the method is simple, and easy to carry out. |
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