| 刘彦华1 雷宁2△ 韩威1 曾庆乐2 刘俊达2.HMGB1 siRNA 对HT-29 细胞侵袭转移能力的影响[J].,2011,11(11):2110-2112 |
| HMGB1 siRNA 对HT-29 细胞侵袭转移能力的影响 |
| Effects of High Mobility Group Box-B1 on Invasive Ability of HT-29 |
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| DOI： |
| 中文关键词: HMGB1 HT-29 侵袭力 |
| 英文关键词: HMGB1 HT-29 Invasive ability |
| 基金项目: |
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| 中文摘要: |
| 目的：观察干扰HMGB1 表达对HT-29 细胞侵袭转移能力的影响。方法：HMGB1 siRNA 通过脂质体转染HT-29 细胞，
western blot 和实时定量RT-PCR 检测HT-29 细胞中HMGB1 蛋白和mRNA 的表达，Transwell 小室观察HT-29 的转移侵袭能力。
结果：干扰HMGB1 后HMGB1 蛋白和mRNA 的表达均减少，HT-29 的转移侵袭能力下降。结论：HMGB1 能促进HT-29 的转移
侵袭能力，干扰其表达可抑制HT-29 的转移侵袭。 |
| 英文摘要: |
| Objective: To study the effect of HMGB1on invasive ability of HT-29. Methods: HT-29 cells were divide into blank
group, control group and HMGB1 siRNA groups. The effects of HMGB1 siRNA on HT-29 cells invasive ability in vitro was measured
by Boyden chamber invasive models. Contents of HMGB1 protein and mRNA were detected by western blot and realtime RT-PCR
methods, respectively. Result: In HMGB1 siRNA group, contents of HMGB1 protein and mRNA were decreased markedly, the invasive
ability was decreased markedly, too. Conclusion: HMGB1 could increase the invasive ability of HT-29 cell. |
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