| 刘媛黄茂△ 李涛刘红.组织贴块法建立人气道平滑肌细胞体外培养模型的研究[J].,2011,11(11):2065-2067 |
| 组织贴块法建立人气道平滑肌细胞体外培养模型的研究 |
| Construction of Model of Human Airway Smooth Muscle in Vitroby Attachment-block Culture |
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| DOI： |
| 中文关键词: 人气道平滑肌 组织贴块法 细胞培养 |
| 英文关键词: Human airway smooth Attachment-block culture Cell culture |
| 基金项目:国家自然科学基金资助（30700342） |
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| 摘要点击次数: 563 |
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| 中文摘要: |
| 背景：人气道平滑肌已被证实参与气道重塑，气道平滑肌的重塑已成为慢性呼吸道疾病的主要病理改变之一。人气道平滑
肌细胞的培养对慢性呼吸道疾病的研究有重要意义。组织贴块法培养人气道平滑肌细胞是原代培养人气道平滑肌细胞的基本方
法之一。目的：采用组织贴块法建立人气道平滑肌体外培养模型。方法：采集人气道组织，用组织贴块法进行人气道平滑肌细胞的
原代培养，获得的细胞经形态学和免疫细胞化学染色鉴定。结果：培养的细胞呈典型的" 谷峰" 状生长,胞浆内特异性的平滑肌肌
动蛋白阳性表达,符合平滑肌细胞的形态学特征和生物学特性。结论：组织贴块法易操作，结果可信，并可培养出高纯度活性好的
人气道平滑肌细胞，成功建立了体外人气道平滑肌细胞增殖模型，提供了研究慢性呼吸道疾病的细胞培养模型。 |
| 英文摘要: |
| Objective: To establish the model of human airway smooth muscle in vitro by attachment-block culture. Methods: The
human airway wall was separated from lung resection carefully and cut into small pieces about 1 mm3, and then these pieces were attached
on the wall of culture bottle. After 3 hours, those tissue pieces were cultivated in DMEM with 20 % calf serum. Results: Cells had
grown on the bottom of culture bottle like peak-valley in shape. All cells almost were smooth muscle cells identified through An- ti-
α-actin immunohistochemistry stain. Conclusion: Attachment-block culture of human airway smooth muscle cells is simple to operate,
reliable in result, high in purity and useful as a cellular model for study of respiratory diseases. |
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