| 高丽娜张荣庆李霜尹志勇范伟伟王海昌曹丰△.Ghrelin 对缺氧复氧状态下脂肪间充质干细胞保护作用的研究[J].,2011,11(5):837-840 |
| Ghrelin 对缺氧复氧状态下脂肪间充质干细胞保护作用的研究 |
| Protection of Ghrelin on Adipose-derived Mesenchymal Stem Cells underHypoxia-reoxygenation Injury |
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| DOI： |
| 中文关键词: Ghrelin 间充质干细胞 缺氧复氧损伤 |
| 英文关键词: Ghrelin Mesenchymal stem cell Hypoxia-reoxygenation |
| 基金项目:国家自然科学基金（30970845）西京医院助推计划重大项目(XJZT08Z04) |
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| 中文摘要: |
| 目的：探讨缺氧复氧损伤环境下Ghrelin 对脂肪来源的间充质干细胞（AD-MSCs）的保护作用，以寻求AD-MSCs 心肌内移
植的有利因素。方法：采用胶原酶消化法分离小鼠AD-MSCs，流式细胞术鉴定其标志。建立缺氧/ 复氧细胞模型，分3 组：①对照
组；②缺氧/ 复氧组（H/R）；③H/R＋Ghrelin（浓度分别为10-9、10-8、10-7mol/L）干预组。MTT 法测定各组细胞增殖，TUNEL 法检测细
胞凋亡。结果：流式细胞术结果显示AD-MSCs CD44 及CD90 阳性，CD34、CD45 阴性。AD-MSCs MTT 分析显示在缺氧环境
中，Ghrelin 相比于单纯H/R 组能够显著促进AD-MSCs 的存活与增殖，并抑制其凋亡（P<0.05）。结论：Ghrelin 可以明显提高缺氧
复氧环境下AD-MSCs 的生存与增殖，抑制缺氧诱导的凋亡发生，有望为心肌梗死的干细胞移植治疗创造新的有利因素。 |
| 英文摘要: |
| Objective: To investigate the effect of Ghrelin on adipose tissue derived mesenchymal stem cells (AD-MSCs) under
hypoxia-reoxygenation (H/R) injury, and to explore the protective factors for myocardial cell transplantation. Methods: AD-MSCs were
isolated from Kunming mice and characterized by flowcytometry. AD-MSCs were divided into (1) Normal control (2) Hypoxia+
Reoxygenation (H/R) (3) H/R+Ghrelin (10-9, 10-8, 10-7 mol/L, respectively). Cell survival and proliferation were assessed by MTT assays.
Apoptosis of AD-MSCs was detected by TUNEL strain. Results: AD-MSCs were positive to the CD44 and CD90. The pretreatment of
Ghrelin improved the survival and proliferation of AD-MSCs under H/R condition compared with that of H/R group (P<0.05). TUNEL
showed Ghrelin inhibited the apoptosis of AD-MSCs after H/R injury (P<0.05). Conclusions: Ghrelin has beneficial effects on the
survival and proliferation of AD-MSCs under H/R environment and might constitute a hopeful target in stem cell transplantation for
ischemic cardiovascular disease. |
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