| 谭飞杨连娟莫小辉樊国彪王秀丽△.UU1、UU3和UU4致昆明小鼠下生殖道致病性的研究[J].,2011,11(3):456-459 |
| UU1、UU3和UU4致昆明小鼠下生殖道致病性的研究 |
| Study on the pathogenicity of UU1, UU3 and UU4 in the lower genitaltract of female KM mice |
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| DOI： |
| 中文关键词: 解脲脲原体 动物模型 致病性 |
| 英文关键词: Ureaplasma urealyticum Animal model Pathogenicity |
| 基金项目:上海市卫生局科研计划课题资助项目（2008Y105），上海皮肤病医院专项业务课题项目 |
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| 中文摘要: |
| 目的：研究解脲脲原体（Ureaplasma Urealyticum，UU）的第1 血清型（UU1）、第3 血清型（UU3）和第4 血清型（UU4）对昆明
小鼠下生殖道的致病性。方法：将100只昆明小鼠随机分为5组：空白组、雌二醇组、UU1组、UU3 组和UU4 组，每组20 只；空白
组和雌二醇组为对照组，UU1 组、UU3 组和UU4 组为实验组；雌二醇组和实验组雌二醇化后1 周，将UU1、UU3 和UU4 分别接
种至UU1、UU3和UU4组小鼠泌尿生殖道，将液体培养基接种至空白组和雌激素组。2 周后检测各组小鼠泌尿生殖道的IL-8、
SIgA、TNF-α并取小鼠子宫组织行病理检查。结果：实验组小鼠UU接种都获得成功。IL-8、SIgA、TNF-α检测结果显示：实验组
和对照组比较有显著性差异；UU4 组和UU1 组及UU3 组比较有显著性差异；空白组和雌二醇组比较无显著性差异；UU1 组和
UU3组比较无显著性差异。病理显示：UU1、UU3和UU4组昆明小鼠子宫组织有炎细胞浸润，UU4组小鼠宫颈病变更明显。对照
组小鼠子宫未见明显改变。结论：UU1、UU3和UU4可能导致昆明小鼠下生殖道产生病理反应；UU4可能短期致病力更强。 |
| 英文摘要: |
| Obsjective: To preliminarily evaluate the pathogenicity of 3 serovars (1,3 and 4) of Ureaplasma Urealyticum(UU1,
UU3 and UU4) in female KM mice's lower genital tract according to the changes of cytokine and pathology results. Methods: Establishment
of animal model: 100 KM mice were randomly divided into control groups (including blank group and estradiol group) and test
groups (including UU1 group, UU3 group and UU4 group).Except for the blank group all the mice received estradiol treatment once a
week. After the second injection of estradiol, UU1, UU3 and UU4 were respectively inoculated intravaginally into groups of UU1, UU3
and UU4 of oestradiol-treated young adult KM strain mice. Liquid culture medium were inoculated intravaginally into control groups. On
the 14th day after inoculation UU tests were taken. Detection of IL-8, SIgA and TNF-α from the mice's lower genital tracts and pathological
examination of mice uterus were performed. Results: UU tests of the control groups were negative and all the test groups were
positive. The levels of IL-8, SIgA and TNF-α in test groups were significantly higher than the control groups, with statistical difference
(P <0.05), and UU4 group were significantly higher than the UU1 and UU3 groups(P <0.05), while blank group and estradiol group, UU1
group and UU3 group had no significant difference (P >0.05). Pathology results: the control groups had no significant change in the
uterus; the test groups showed interstitial infiltration of polymorphonuclear leukocyte in the uterus and the pathological change of UU4
group were worse. Conclusions: Serovars (1,3 and 4) of Ureaplasma Urealyticum may committ pathogenicity in the lower genital tract of
female KM mices and the disorder caused by UU4 may be more severe in short duration. |
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