| 陈赵慧,杨今言,李丽华,李 琳,高雪雯.miR-27a靶向FOXG1调控皮肤鳞状细胞癌细胞增殖、侵袭及迁移的研究[J].现代生物医学进展英文版,2024,(13):2428-2433. |
| miR-27a靶向FOXG1调控皮肤鳞状细胞癌细胞增殖、侵袭及迁移的研究 |
| miR-27a Targets FOXG1 to Regulate Proliferation, Invasion and Migration of Cutaneous Squamous Cell Carcinoma Cells |
| Received:December 27, 2023 Revised:January 24, 2024 |
| DOI：10.13241/j.cnki.pmb.2024.13.005 |
| 中文关键词: miR-27a FOXG1 皮肤鳞状细胞癌 侵袭 |
| 英文关键词: miR-27a FOXG1 Skin squamous cell carcinoma Invasion |
| 基金项目:新疆维吾尔自治区科技支疆计划项目（2019E0289） |
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| 中文摘要: |
| 摘要 目的：探讨miR-27a对皮肤鳞状细胞癌（CSCC）细胞增殖、侵袭、迁移的影响及其与叉头框G1（FOXG1）的靶向关系。方法：收集30例CSCC组织及30例正常皮肤组织。培养A431细胞，将miR-NC、miR-27a mimics、si-NC、si-miR-27a、Scramble、si-FOXG1、Vector、OE-FOXG1质粒分别转染至细胞，记为miR-NC组、miR-27a组、si-NC组、si-miR-27a组、Scramble组、si-FOXG1组、Vector组及FOXG1组；将si-FOXG1、Scramble质粒分别转染至miR-27a组细胞，记为miR-27a+Scramble组、miR-27a+si-FOXG1组。四甲基偶氮唑蓝（MTT）检测细胞增殖能力，Transwell实验检测细胞侵袭、迁移能力，荧光定量聚合酶链反应（RT-PCR）检测细胞、组织中FOXG1基因mRNA及miR-27a表达水平，Western blot检测细胞或组织FOXG1蛋白表达水平，TargetScan在线网站预测miR-27a与FOXG1结合位点，双荧光素酶报告基因实验验证miR-27a与FOXG1的靶向关系。结果：CSCC组织FOXG1基因mRNA、miR-27a表达水平高于正常皮肤组织（P<0.05），CSCC组织FOXG1基因mRNA、miR-27a表达水平呈正相关（r=0.801，P=0.000）。上调miR-27a、FOXG1可促进细胞的增殖、侵袭、迁移，沉默miR-27a、FOXG1可抑制细胞的增殖、侵袭、迁移及EMT。下调FOXG1逆转了过表达miR-27a对细胞的增殖、侵袭、迁移的促进作用。miR-27a可正性调控FOXG1表达。结论：miR-27a、FOXG1在CSCC组织中高表达，miR-27a正性调控FOXG1促进CSCC细胞的增殖、侵袭、迁移。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of miR-27a on Proliferation, invasion and migration of cutaneous squamous cell carcinoma (CSCC) cells and its targeting relationship with forkhead box G1 (FOXG1). Methods: 30 cases CSCC tissues and 30 cases normal skin tissues were collected. A431 cells were cultured. miR-NC, miR-27a mimics, si-NC, si-miR-27a, Scramble, si-FOXG1, Vector, and OE-FOXG1 plasmids were transfected into the cells and defined as miR-NC group, miR-27a group, si-NC group, si-miR-27a group, Scramble group, si-FOXG1 group, Vector group and FOXG1 group. The si-FOXG1 and Scramble plasmids were transfected into the cells of miR-27a group and recorded as miR-27a+Scramble group and miR-27a+si-FOXG1 group. The cell Proliferation ability was detected by tetramethylazole blue (MTT). The cell invasion and migration ability were detected by Transwell assays. FOXG1 gene mRNA and miR-27a level were detected by fluorescence quantitative polymerase chain reaction (RT-PCR). FOXG1 protein level were detected by Western blot. TargetScan online website was used to predict the binding site of miR-27a and FOXG1. Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-27a and FOXG1. Results: The levels of FOXG1 gene mRNA and miR-27a in CSCC tissues were higher than normal skin tissues (P<0.05), and the levels of FOXG1 gene mRNA in CSCC tissues were positively correlated with and miR-27a level (r=0.801, P=0.000). Up-regulation of miR-27a and FOXG1 promoted cell proliferation, invasion and migration, and silencing of miR-27a and FOXG1 inhibited cell proliferation, invasion, migration and EMT. Down-regulation of miR-27a reversed the the promotive effect of cell Proliferation, invasion and migration by overexpression of FOXG1. miR-27a positively regulated FOXG1 expression. Conclusion: miR-27a and FOXG1 are highly expressed in CSCC tissues. miR-27a positively regulates FOXG1 to promote proliferation, invasion, and migration of CSCC cells. |
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