| 杨凯宁,卜莉萍,李桂丽,段丽丽,潘红春.强光及高温诱发白化的中国绿水螅共生细菌多样性的特征[J].现代生物医学进展英文版,2024,(8):1401-1410. |
| 强光及高温诱发白化的中国绿水螅共生细菌多样性的特征 |
| The Diversity of Symbiotic Bacteria of Hydra sinensis Undergoing Bleaching Induced by Strong Light and High Temperature |
| Received:November 28, 2023 Revised:December 23, 2023 |
| DOI：10.13241/j.cnki.pmb.2024.08.001 |
| 中文关键词: 中国绿水螅 共生藻 白化 共生细菌 抗氧化能力 |
| 英文关键词: Hydra sinensis Symbiotic algae Bleaching Symbiotic bacteria Antioxidant capacity |
| 基金项目:国家自然科学基金项目（42077208；31671529） |
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| 中文摘要: |
| 摘要 目的：探究强光及高温诱发白化的中国绿水螅共生细菌多样性的特征。方法：以光照强度2000 Lux和温度25℃条件为对照，分别在强光胁迫（6000 Lux，25℃）、高温胁迫（2000 Lux，33℃）以及强光和高温联合胁迫（6000 Lux，33℃）等3种条件下进行诱导中国绿水螅白化的实验。白化实验进行到21天后，采用对单个水螅细胞中的共生藻进行计数的方法检测绿水螅白化的程度，同时通过16S rDNA 高通量测序的方法分析绿水螅共生细菌多样性及丰度变化。结果：在强光胁迫、高温胁迫及强光和高温联合胁迫下绿水螅体内共生藻数量均显著减少（P<0.05）。强光胁迫下绿水螅共生细菌中蓝细菌门（Cyanobacteria）的丰度上升，而在强光和高温联合胁迫下绿水螅共生细菌中不但蓝细菌门的丰度上升，绿硫菌门（Chlorobi）的丰度也明显提高。此外，KEGG富集分析结果表明发生白化的绿水螅共生细菌与亚油酸、类胡萝卜素及甜菜红色素等抗氧化剂的合成和代谢相关。结论：蓝细菌和绿硫菌的光合作用产物有可能被水螅宿主所利用、以弥补水螅宿主由于共生藻的减少带来的营养缺乏；强光和高温胁迫下绿水螅出现白化现象后共生细菌多样性朝有利于提高水螅宿主抗氧化能力的方向发生变化。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the symbiotic bacterial diversity of Hydra sinensis undergoing bleaching induced by strong light and high temperature. Methods: The bleaching of H. sinensis was induced under strong light (6000 Lux, 25℃), high temperature (2000 Lux, 33℃) and combined stresses of strong light and high temperature (6000 Lux, 33℃), respectively. After 21 days of bleaching experiment, the bleaching degree of H. sinensis was detected by counting symbiotic algae in single cells of H. sinensis, and the diversity and abundance of symbiotic bacteria in H. sinensis were studied by use of high-throughput sequencing of 16S rDNA. Results: The number of symbiotic algae was observably decreased under strong light, high temperature and combined stresses of strong light and high temperature (P<0.05). The abundance of Phylum Cyanobacteria in H. sinensis increased under strong light, while the abundances of Phylum Cyanobacteria and Chlorobi in H. sinensis increased significantly under the combined stresses of strong light and high temperature. In addition, KEGG enrichment analysis indicated that symbiotic bacteria in H. sinensis undergoing bleaching were related to the biosynthesis and metabolism of antioxidants such as linoleic acid, carotenoid and betalain. Conclusion: Cyanobacteria and chloracea may provide their photosynthetic products to the host of H. sinensis to make up for the nutritional deficiency caused by the decrease of symbiotic algae. Furthermore, the diversity of symbiotic bacteria in H. sinensis changed in the direction of improving the antioxidant capacity of the host under strong light and/or high temperature stress. |
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