| 骆小梅,曹 冰,曹 婷,胡雁飞,薄 涛.灵芝酚通过AGE/RAGE信号通路对慢性肾脏病大鼠炎症反应及肾脏纤维化的影响[J].现代生物医学进展英文版,2024,(7):1235-1239. |
| 灵芝酚通过AGE/RAGE信号通路对慢性肾脏病大鼠炎症反应及肾脏纤维化的影响 |
| Effects of Ganoderma Lucidum Phenol on Inflammatory Response and Renal Fibrosis in Rats with Chronic Kidney Disease through AGE / RAGE Signaling Pathway |
| Received:November 09, 2023 Revised:November 30, 2023 |
| DOI：10.13241/j.cnki.pmb.2024.07.006 |
| 中文关键词: 慢性肾脏病大鼠 灵芝酚 AGE/RAGE信号通路 炎症反应 肾脏纤维化 |
| 英文关键词: Chronic kidney disease rats Ganoderma lucidum phenol aGE / RAGE signaling pathway Inflammatory response Renal fibrosis |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2021D01C441）；新疆维吾尔自治区自然科学基金项目（2020D01C219） |
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| 中文摘要: |
| 摘要 目的：探讨灵芝酚通过AGE/RAGE信号通路对慢性肾脏病（CKD）大鼠炎症反应及肾脏纤维化的影响。方法：将40只SD雄性大鼠分为模型组、灵芝酚低剂量组、灵芝酚中剂量组、灵芝酚高剂量组，另选取健康大鼠设空白对照组，每组10只。空白对照组、模型组均注射等容量生理盐水，灵芝酚低、中、高剂量组分别给予5、10、20 mg/kg灵芝酚。给药结束后采用免疫透射比浊法检测肾功能；采用酶联免疫吸附法检测炎症因子和肾脏纤维化；并称大鼠体质量、测定肾脏质量计算肾脏指数，评估肾脏病变评分。结果：模型组、灵芝酚（低、中、高剂量）组血肌酐（Scr）、尿素氮（BUN）、尿酸（UA）水平均高于空白对照组；灵芝酚（低、中、高剂量）组BUN、SCr、UA水平均高于模型组（P＜0.05）。与空白对照组比较，模型组、灵芝酚（低、中、高剂量）组体质量较低，肾脏指数、病变评分较高；与模型组比较，灵芝酚（低、中、高剂量）组体质量较高，肾脏指数、病变评分较低（P＜0.05）。血清C反应蛋白（CRP）、白介素-6（IL-6）、白介素-8（IL-8）、肿瘤坏死因子-?琢（TNF-?琢）比较，模型组、灵芝酚（低、中、高剂量）组均高于空白对照组；灵芝酚（低、中、高剂量）组均低于模型组（P＜0.05）。血清透明质酸（HA）、Ⅳ型胶原（C-Ⅳ）、层黏蛋白（LN）、Ⅲ型前胶原（PCⅢ）水平比较，模型组、灵芝酚（低、中、高剂量）组均高于空白对照组；灵芝酚（低、中、高剂量）组均低于模型组（P＜0.05）。结论：灵芝酚通过AGE/RAGE信号通路可有效抑制炎症反应和肾脏纤维化，同时还能改善肾功能，对CKD大鼠起到一定保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of ganoderma lucidum phenol on inflammatory response and renal fibrosis in rats with chronic kidney disease (CKD) through AGE / RAGE signaling pathway. Methods: Forty SD male rats were divided into model group, low-dose ganoderma lucidum phenol group, medium-dose ganoderma lucidum phenol group and high-dose ganoderma lucidum phenol group. In addition, healthy rats were selected as blank control group, with 10 rats in each group. The blank control group and the model group were injected with equal volume of normal saline, and the low, medium and high dose groups of ganoderma lucidum phenol were given 5,10,20 mg / kg ganoderma lucidum phenol respectively. After administration, renal function was detected by immunoturbidimetry. Enzyme-linked immunosorbent assay was used to detect inflammatory factors and renal fibrosis; the body weight of the rats was weighed, the kidney weight was measured, the kidney index was calculated, and the renal lesion score was evaluated. Results: The levels of serum creatinine (Scr), urea nitrogen (BUN) and uric acid (UA) in the model group, Ganoderma lucidum phenol (low, medium and high dose) groups were higher than those in the blank control group; the levels of BUN, SCr and UA in the ganoderma lucidum phenol (low, medium and high dose) groups were higher than those in the model group (P<0.05). Compared with the blank control group, the body weight of the model group and the ganoderma lucidum phenol (low, medium and high dose) group was lower, and the kidney index and lesion score were higher. Compared with the model group, the body weight of the ganoderma lucidum phenol (low, medium and high dose) groups was higher, and the kidney index and lesion score were lowe (P<0.05). Compared with serum C-reactive protein (CRP), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α), the model group and ganoderma lucidum phenol (low, medium and high dose) groups were higher than the blank control group; the ganoderma lucidum phenol (low, medium and high dose) groups were lower than the model group (P<0.05). The levels of serum hyaluronic acid (HA), type IV collagen (C-IV), laminin (LN) and type III procollagen (PCIII) in the model group and the ganoderma lucidum phenol (low, medium and high dose) groups were higher than those in the blank control group. The ganoderma lucidum phenol (low, medium and high dose) groups were lower than the model group (P<0.05). Conclusion: Ganoderma lucidum phenol can effectively inhibit inflammatory response and renal fibrosis through AGE / RAGE signaling pathway, and can also improve renal function and play a protective role in CKD rats. |
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