| 回雪颖,郭伟光,王 景,张海丽,刘 颖,马铭堃.全蝎软膏对糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体表达的影响[J].现代生物医学进展英文版,2024,(4):630-635. |
| 全蝎软膏对糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体表达的影响 |
| Effects of Scorpion Ointment on EGF, PDGF and their Receptors Expression in Diabetic Skin Ulcer Rat Model |
| Received:June 29, 2023 Revised:July 31, 2023 |
| DOI：10.13241/j.cnki.pmb.2024.04.006 |
| 中文关键词: 全蝎软膏 糖尿病皮肤溃疡 表皮生长因子 血小板衍生生长因子 |
| 英文关键词: Scorpion ointmen Diabetic skin ulcer Epidermal growth factor Platelet-derived growth factor |
| 基金项目:黑龙江省卫健委项目（20210404130180）；黑龙江省中医药管理局项目（ZHY2020-142）；黑龙江中医药大学附属第二医院孙申田青年人才基金项目（2020KY-02） |
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| 中文摘要: |
| 摘要 目的：探讨全蝎软膏对糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体表达的影响及创面愈合的机理。方法：60只大鼠随机分为对照组、模型组、湿润烧伤膏组和全蝎软膏组，每组15只。建立糖尿病皮肤溃疡大鼠模型，干预后7天及14天，观察并比较大鼠创面愈合率和血清中表皮生长因子(epidenllal giiilllh factor, EGF)、血小板衍生生长因子(platelet -derived growth factor, PDGF)及其受体水平。结果：全蝎软膏组、湿润烧伤膏组与模型组7 d、14 d同时间点比较创面愈合率，差异有统计学意义(P＜0.05或 P＜0.01)；全蝎软膏组、湿润烧伤膏组7 d、14 d同时间点比较，差异无统计学意义(P＞0.05)。免疫组化结果显示，EGF的表达水平，全蝎软膏组、湿润烧伤膏组与模型组比较，7 d差异有统计学意义(P＜0.05)；14 d差异无统计学意义(P＞0.05)。EGFR 的表达水平，全蝎软膏组与湿润烧伤膏组、模型组、空白组7 d、14 d同时间点比较，差异无统计学意义(P＞0.05)。PDGFB的表达水平，全蝎软膏组、湿润烧伤膏组与模型组7 d、14 d同时间点比较，差异有统计学意义(P＜0.05)。PDGFRB的表达水平，7 d全蝎软膏组与湿润烧伤膏组、模型组、空白组比较，差异无统计学意义(P＞0.05)；14 d全蝎软膏组、湿润烧伤膏组与模型组比较，差异有统计学意义(P＜0.05或P＜0.01)。结论：全蝎软膏可影响糖尿病皮肤溃疡大鼠模型EGF、PDGF及其受体的表达，促进糖尿病创面愈合。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect and the mechanism of wound healing of Scorpion ointment on the expression of EGF, PDGF and their receptors in diabetic skin ulcer rat model. Methods: Sixty rats were randomly divided into control group, model group, MEBO group and scorpion ointment group, with 15 rats in each group.Diabetic skin ulcer rat model was established, 7 and 14 days after intervention, wound healing rate and Levels of epidermal growth factor (EGF), platelet -derived growth factor (PDGF) and their receptors in serum were observed and compared. Results: The wound healing rates of scorpion ointment group, MEBO group and model group were compared at the same time point at 7 d and 14 d, and the difference was statistically significant(P＜0.05 or P＜0.01)；there was no significant difference between scorpion ointment group and MEBO group at 7 d and 14 d at the same time point(P＞0.05).Immunohistochemical results showed that there were statistically significant differences in the expression levels of EGF between scorpion ointment group, MEBO group and model group at 7 d(P<0.05), but there was no statistically significant difference at 14 d (P>0.05). There was no significant difference in the expression level of EGFR between scorpion ointment group and MEBO group, model group and blank group at 7 d and 14 d at the same time point(P>0.05). The expression level of PDGFB in scorpion ointment group, MEBO group and model group was statistically significant at the same time point on day 7 and day 14(P<0.05). There was no significant difference in the expression level of PDGFRB between scorpion ointment group and MEBO group, model group, blank group(P>0.05), but there was statistical significance at 14 d scorpion ointment group and MEBO group compared with model group(P<0.05 or P<0.01). Conclusion: Scorpion ointment can affect the expression of EGF, PDGF and their receptors in diabetic skin ulcer rat model, and promote the healing of diabetic wound. |
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