| 徐境阳,王 娴,雷 华,周 伟,王 军.降气化痰推拿法对慢性哮喘幼鼠气道重塑及血清NO水平的影响[J].现代生物医学进展英文版,2024,(3):423-427. |
| 降气化痰推拿法对慢性哮喘幼鼠气道重塑及血清NO水平的影响 |
| Effect of Qi-descending and Phlegm-resolving Massage on Airway Remodeling and Serum NO Level in Young Rats with Chronic Asthma |
| Received:September 07, 2023 Revised:September 30, 2023 |
| DOI：10.13241/j.cnki.pmb.2024.03.005 |
| 中文关键词: 哮喘 降气化痰 炎性反应 Th17/Treg NO 支气管肺泡灌洗液 |
| 英文关键词: Chronic Asthma Qi-descending and phlegm-resolvin Inflammatory response Th17/Treg NO BALF |
| 基金项目:新疆维吾尔自治区自然科学基金青年项目（2021D01C350） |
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| 中文摘要: |
| 摘要 目的：探究降气化痰推拿法对慢性哮喘幼鼠的治疗效果，为优化临床中小儿慢性哮喘治疗方案积累理论基础。方法：选取40只SPF级3周SD雌性小鼠，随机数字表法为对照组、模型组、药物组和推拿组，每组各10只。模型组、药物组和推拿制备慢性哮喘模型后，药物组使用地塞米松治疗，对照组注射等量生理盐水，推拿组在药物组基础上加入降气化痰推拿手法治疗，模型组不进行干预。比较各组幼鼠肺组织HE染色结果、支气管肺泡灌洗液（BALF）中炎性细胞水平和Th17/Treg细胞比例，并检测肺组织中Treg与Th17相关基因表达量。结果：与模型组比较，药物组和推拿组的支气管周围组织的炎性浸润较轻，肺泡壁及支气管壁的结构较为完整，气道可见少量杯状细胞增生，气道壁总厚度和气道平滑肌厚度增加较少，且推拿组大鼠的组织炎性浸润程度、气道壁总厚度和气道平滑肌厚度均低于药物组（P<0.05）；炎性细胞水平方面，对照组幼鼠的BALF细胞总数较低，白细胞分类中单核细胞数最多。模型组幼鼠的BALF中细胞总数明显增高，且白细胞分类中以中性粒细胞、嗜酸性粒细胞为主，白细胞总数和嗜酸性细胞总数明显高于对照组（P<0.05）；与模型组相比，药物组和推拿组的细胞总数及中性粒细胞和嗜酸性粒细胞的比例明显较低，但仍明显高于对照组，且药物治疗组高于推拿组（P<0.05）；与对照组相比，其余各组幼鼠的BALF中Th17比例明显增高，Treg比例降低，Th17/Treg比值增高（P<0.05）；与模型组相比，药物组和推拿组的Th17比例、Th17/Treg降低，且推拿组低于药物组，Treg比例升高，且推拿组高于药物组（P<0.05）；与对照组对比，其余各组幼鼠的IL-6、IL-10、IL-23、TGF-β mRNA表达水平均升高，与模型组相比，药物组与推拿组幼鼠表达水平均有所降低，且推拿组低于药物组（P<0.05）；与对照组相比，其余各组幼鼠的NO、IgE水平升高，与模型组相比，药物组和推拿组的NO、IgE水平降低，且推拿组低于药物组（P＜0.05）。结论：降气化痰推拿法可通过调节Th17/Treg比例及相关炎性因子基因表达和白细胞类型，调控血清NO、IgE水平和炎性反应水平，进而改善气道重塑，发挥治疗作用。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the therapeutic effect of qi-descending and phlegm-resolving massage on young mice with chronic asthma, and to accumulate theoretical basis for optimizing clinical treatment of chronic asthma in children. Methods: 40 SPF grade 3-week SD female mice were selected and divided into control group, model group, drug group and massage group with 10 mice in each group by random number table method. After the model group, the drug group and the massage group were modeled as chronic asthma model, the drug group was treated with dexamethasone, the control group was injected with the same amount of normal saline, the massage group was added to the drug group on the basis of qi-descending and phlegm-resolving massage treatment, and the model group did not intervene. The results of HE staining, inflammatory cell level and Th17/Treg cell ratio in Bronchoalveolar Lavage Fluid (BALF) were compared in lung tissues of all groups, and the expression levels of Treg and TH17-related genes in lung tissues were detected. Results: Compared with the model group, the inflammatory infiltration of peribronchial tissue and massage group is light, the structure of the alveolar wall and bronchial wall is relatively complete, a small amount of goblet cell hyperplasia in the airway, the airway wall thickness and airway smooth muscle thickness increased less, and the tissue inflammatory infiltration, the total thickness and airway smooth muscle thickness are lower than the drug group (P<0.05); Regarding the inflammatory cell levels, the control pups had a lower total number of BALF cells and the highest number of monocytes in the leukocyte classification. The total number of cells in the BALF of the model group was significantly higher, and neutrophils and eosinophils were mainly included in the leukocyte classification, and the total number of leukocytes and eosinophils were significantly higher than that of the control group(P<0.05); Compared with the model group, the total number of cells and the proportion of neutrophils and eosinophils were significantly lower in the drug group and the massage group, but still significantly higher than the control group, and the drug treatment group was higher than the massage group (P<0.05); Compared with the control group, the proportion of Th 17, the proportion of Treg decreased and the ratio of Th 17 / Treg increased in the BALF (P<0.05); Compared with the model group, the proportion of Th 17 and Th 17 / Treg decreased in the drug group and the massage group, and the Treg ratio was lower than the drug group, and the Treg ratio was higher than the massage group (P<0.05); Compared with the control group, the expression levels of IL-6, IL-10, IL-23 and TGF-β mRNA in the other groups were increased, and compared with the model group, the expression levels of the drug group and the massage group were reduced, and the massage group was lower than the drug group (P<0.05); Compared with the control group, NO and IgE levels were increased, NO and IgE levels were decreased in the drug group and the massage group compared with the model group, and the massage group was lower than the drug group (P<0.05). Conclusion: Qi-descending and phlegm-resolving massage, thus improving airway remodeling and playing a therapeutic role by regulating Th17/Treg ratio, expression of related inflammatory factors and types of white blood cells, the serum levels of NO, IgE, and inflammatory response. |
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