| 薛松妍,薛 强,余楠楠,王雪颖,王睿琪,马 静.芪丹通脉片对阿霉素诱导扩张型心肌病大鼠lncRNA XIST表达的影响[J].现代生物医学进展英文版,2024,(2):211-218. |
| 芪丹通脉片对阿霉素诱导扩张型心肌病大鼠lncRNA XIST表达的影响 |
| Effect of Qidantongmai Tablet on lncRNA XIST Expression in Rats with Adriamycin Induced Dilated Cardiomyopathy |
| Received:July 19, 2023 Revised:August 16, 2023 |
| DOI：10.13241/j.cnki.pmb.2024.02.003 |
| 中文关键词: 芪丹通脉片 阿霉素 扩张型心肌病 lncRNA XIST 细胞凋亡 纤维化 |
| 英文关键词: Qidantongmai tablet Adriamycin Dilated cardiomyopathy LncRNA XIST Apoptosis Fibrosis |
| 基金项目:陕西省中医药管理局科研立项课题（2021-ZZ-JC015）；西安交通大学机械制造系统工程国家重点实验室开放课题（sklms2021013） |
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| 中文摘要: |
| 摘要 目的：探究芪丹通脉片（QDTM）对阿霉素诱导扩张型心肌病（DCM）大鼠的治疗作用及其对长链非编码RNA（lncRNA）X-无活性特异性转录物（XIST）表达的影响。方法：将大鼠分为Con组（n=12）、DCM组（n=13）、L-QDTM组（n=13）、M-QDTM组（n=13）、H-QDTM组（n=13）。Con组大鼠为正常对照大鼠，其他组大鼠均为阿霉素诱导的扩张型心肌病模型大鼠。Con组和DCM组大鼠灌胃生理盐水，L-QDTM组、M-QDTM组和H-QDTM组大鼠分别灌胃500、1000和2000 mg/kg/d的芪丹通脉片浸膏干粉，每日给药1次，共4周。治疗结束后，分别检测各组大鼠的心功能参数，血清心肌损伤指标和心肌组织氧化应激指标。通过心肌组织苏木素伊红（HE）染色、Masson三色染色和TUNEL染色观察心肌形态变化、纤维化和细胞凋亡情况。通过RT-qPCR检测心肌组织中XIST、collagen I、collagen Ⅲ、TGF-β1、Bax和Bcl-2的转录水平。结果：与Con组比较，DCM组大鼠的左室射血分数（LVEF）和左室短轴缩短率（FS）降低，左室舒张末期内径（LVIDd）和左心室收缩末期内径（LVIDs）升高，乳酸脱氢酶（LDH）、肌酸激酶（CK）和心肌肌钙蛋白I（cTnI）升高；心肌出现明显损伤，纤维化面积升高；collagen I、collagen Ⅲ和TGF-β1的mRNA水平均升高，TUNEL阳性率升高；Bax mRNA水平升高，Bcl-2 mRNA水平降低，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平降低，丙二醛（MDA）水平升高，XIST水平升高（均P<0.05）。与DCM组比较，L-QDTM组、M-QDTM组和H-QDTM组的LVEF和FS均升高，LVIDd和LVIDs均降低，LDH、CK和cTnI均降低；心肌损伤减轻，纤维化面积降低；collagen I、collagen Ⅲ和TGF-β1的mRNA水平均降低，TUNEL阳性率降低；Bax mRNA水平降低，Bcl-2 mRNA水平升高，SOD和CAT水平升高，MDA水平均降低，XIST水平降低（均P<0.05）。结论：本研究表明芪丹通脉片在治疗阿霉素诱导扩张型心肌病大鼠中效果显著，其机制可能与抑制lncRNA XIST有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the therapeutic effect of Qidantongmai tablet (QDTM) on adriamycin-induced dilated cardiomyopathy (DCM) in rats and its effect on the expression of long non-coding RNA (lncRNA) X-inactive specific transcript (XIST). Methods: Rats were divided into Con group (n=12), DCM group (n=13), L-QDTM group (n=13), M-QDTM group (n=13), and H-QDTM group (n=13). Rats in Con group rats were normal control, and rats in other groups were doxorubicin-induced dilated cardiomyopathy model rats. Rats in Con group and DCM group were given normal saline, rats in L-QDTM group, M-QDTM group and H-QDTM group were given QDTM extractum powder of 500, 1000 and 2000 mg/kg/d, respectively. The rats of each group were given the drug once a day for 4 weeks. After treatment, The cardiac function parameters, serum myocardial injury indexes and myocardium tissue oxidative stress indicators of each group were detected, respectively. Myocardial morphology, fibrosis and apoptosis were observed by hematoxylin eosin (HE), Masson tricolor and TUNEL staining. The transcription levels of XIST, collagen I, collagen Ⅲ, TGF-β1, Bax and Bcl-2 in myocardial tissue were detected by RT-qPCR. Results: Compared with Con group, left ventricular ejection fraction (LVEF) and left ventricular short-axis shortening rate (FS) in DCM group decreased, left ventricular end-diastolic diameter (LVIDd) and left ventricular end-systolic diameter (LVIDs) increased, lactate dehydrogenase (LDH), creatine kinase (CK) and cardiac troponin I (cTnI) increased, myocardial injury was obvious, fibrosis area increased, collagen I, collagen Ⅲ and TGF-β1 mRNA levels increased, TUNEL positive rate increased, Bax mRNA level increased, Bcl-2 mRNA level decreased, superoxide dismutase (SOD) and catalase (CAT) levels decreased, malondialdehyde (MDA) level increased, and XIST level increased (all P<0.05). Compared with DCM group, LVEF and FS in L-QDTM group, M-QDTM group and H-QDTM group increased, LVIDd and LVIDs decreased, LDH, CK and cTnI decreased, myocardial injury was alleviated, fibrosis area decreased, collagen I, collagen Ⅲ and TGF-β1 mRNA levels decreased, TUNEL positive rate decreased, Bax mRNA level decreased, Bcl-2 mRNA level increased, SOD and CAT levels increased, MDA level decreased, and XIST level decreased (all P<0.05). Conclusion: This study indicates that Qidantongmai tablet is effective in the treatment of doxorubicin-induced dilated cardiomyopathy in rats, and the mechanism may be related to the inhibition of lncRNA XIST. |
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