| 杜小琴,王燕侠,李 霞,马巨星,曹 婷.血清IL-18、IL-1β及NLRP3炎症小体在射血分数保留型心衰患者中的表达及其与肠道菌群的相关性分析[J].现代生物医学进展英文版,2023,(23):4492-4495. |
| 血清IL-18、IL-1β及NLRP3炎症小体在射血分数保留型心衰患者中的表达及其与肠道菌群的相关性分析 |
| Expression of Serum IL-18, IL-1β and NLRP3 Inflammasome Bodies in Patients with Ejection Fraction Retention Heart Failure and Their Correlation with Intestinal Flora |
| Received:April 07, 2023 Revised:May 10, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.23.018 |
| 中文关键词: IL-18 IL-1β NLRP3炎症小体 射血分数保留型心衰 肠道菌群 |
| 英文关键词: IL-18 IL-1β NLRP3 inflammasome Ejection fraction retention heart failure Intestinal flora |
| 基金项目:新疆维吾尔自治区健康青年医学科技人才专项科研项目（WJWY-202145） |
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| 中文摘要: |
| 摘要 目的：血清IL-18、IL-1β及NLRP3炎症小体在射血分数保留型心衰患者中的表达及其与肠道菌群的相关性分析。方法：选择2020年5月到2022年1月于我院住院治疗的50例射血分数保留型心衰患者作为实验组，选取同期健康体检者50例作为对照组，收集两组人口学资料、一般查体资料及心脏彩超等结果。检测血清IL-18、IL-1β、NLRP3、含半胱氨酸的天冬氨酸蛋白酶1（Caspase-1）表达水平。采用每克粪便湿质量中菌落形成单位对数值（1gCFU/g），给予活菌计数，肠道定植抗力指标则用双歧杆菌与肠杆菌数量的比值(B/E值)表示。结果：（1）实验组IL-18、IL-1β表达水平较对照组明显升高（P<0.05）；（2）实验组NLRP3、Caspase-1表达水平较对照组明显升高（P<0.05）；（3）实验组的双歧杆菌、乳杆菌数目和B/E值较对照组低，肠球菌、肠杆菌数目较对照组高（P<0.05）；（4）两组的Chao1指数无差异；病例组Shannon指数较对照组低，Simpson指数较对照组高（P<0.05）；（5）Pearson分析显示，射血分数保留型心衰患者B/E值与IL-18、IL-1β及NLRP3炎症小体表达正相关。结论：射血分数保留型心衰患者存在血清IL-18、IL-1β及NLRP3炎症小体表达明显升高及肠道菌群失调，且IL-18、IL-1β及NLRP3炎症小体与肠道菌群有显著相关性。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of serum IL-18, IL-1β and NLRP3 inflammasome in patients with ejection fraction retention heart failure and their correlation with intestinal flora. Methods: 50 patients with preserved ejection fraction heart failure hospitalized in our hospital from May 2020 to January 2022 were selected as the experimental group, and 50 healthy examination patients in the same period were selected as the control group to collect the two groups of demographic data, general physical examination data and cardiac color ultrasound results. Serum expression levels of IL-18, IL-1β, NLRP 3, and aspartate-containing protease 1 (Caspase-1) were measured. The log (1 g CFU / g), and the intestinal colonization resistance index was expressed as the ratio of bifidobacterium and Enterobacterium (B / E value). Results: (1) The expression levels of IL-18 and IL-1β in the experimental group were significantly higher than that in the control group (P<0.05). (2) The expression levels of NLRP3 and Caspase-1 in the experimental group were significantly higher than that in the control group (P<0.05). (3) The number of bifidobacterium and Lactobacillus and B/E value in the experimental group were significantly lower than that in the control group, while the number of enterococcus and Enterobacter were significantly higher than that in the control group (P<0.05). (4) There is no significant difference in Chao1 index between the two groups; The Shannon index of the case group was significantly lower than that of the control group, and the Simpson index was significantly higher than that of the control group (P<0.05). (5) Pearson analysis showed that B/E values in patients with ejection fraction preserved heart failure were positively correlated with IL-18, IL-1β and NLRP3 inflammasome expression. Conclusion: The expression of serum IL-18, IL-1β and NLRP3 inflammasome weresignificantly increased in patients with ejection fraction preserved heart failure, and the intestinal flora is dysregulated, and IL-18, IL-1β and NLRP3 inflammasome were significantly correlated with intestinal flora. |
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