| 杨柏琦,齐素兰,马金叶,郭中华,史栋梁.补肝健腰方对于腰椎间盘退变模型组织中bFGF、TGF-β1、BMP-3表达影响的实验研究[J].现代生物医学进展英文版,2023,(22):4235-4240. |
| 补肝健腰方对于腰椎间盘退变模型组织中bFGF、TGF-β1、BMP-3表达影响的实验研究 |
| Experimental Study on the Effect of Bugan Jianyao Decoction on the Expression of bFGF, TGF-β1 and BMP-3 in the Tissue of Lumbar Disc Degeneration Model |
| Received:May 10, 2023 Revised:May 31, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.22.007 |
| 中文关键词: 补肝健腰方 腰椎间盘退变 bFGF TGF-β1 BMP-3 |
| 英文关键词: Bugan Jianyao decoction Lumbar disc degeneration bFGF TGF-β1 BMP-3 |
| 基金项目:2023年度河南省中医药科学研究专项（2023ZY2097） |
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| 中文摘要: |
| 摘要 目的：探讨补肝健腰方对大鼠腰椎间盘退变模型中碱性成纤维细胞生长因子（bFGF）、转化生长因子-β1（TGF-β1）、骨形态发生蛋白-3（BMP-3）表达的影响。方法：取SD大鼠90只，随机分为正常对照组、假手术组、模型组、低、中、高剂量补肝健腰方组，每组各15只，正常对照组不予处理，假手术组仅暴露椎间盘而不做椎间盘穿刺，余四组制备大鼠腰椎间盘退变模型，造模成功后，分别给予低、中、高剂量补肝健腰方组低、中、高剂量补肝健腰方药液灌胃；正常对照组、假手术组、模型组给予等量生理盐水灌胃，检测各组干预前、20 d后、40 d后椎间盘TGF-β1、bFGF mRNA及BMP-3含量。结果：与正常对照组及假手术组相比，模型组及补肝健腰方各剂量组干预前TGF-β1、bFGF mRNA及BMP-3的表达均上升（P＜0.05） , 干预20 d、40 d后补肝健腰方各剂量组TGF-β1、bFGF mRNA及BMP-3的表达上升（P＜0.05）。与模型组相比，补肝健腰方各剂量组干预前TGF-β1、bFGF mRNA及BMP-3的含量未见明显变化（P>0.05），干预20 d后补肝健腰方各剂量TGF-β1、bFGF mRNA及BMP-3的表达均下降（P＜0.05），干预40 d后补肝健腰方高剂量组与中剂量组TGF-β1、bFGF mRNA及BMP-3的表达下降更为明显（P＜0.05），低剂量组中TGF-β1、bFGF mRNA及BMP-3的表达也下降（P＜0.05）。干预40 d后补肝健腰方高剂量组、中剂量组较低剂量组TGF-β1、bFGF mRNA及BMP-3的表达下降更为明显(P＜0.05)。结论：补肝健腰方能降低大鼠腰椎间盘退变模型中bFGF、TGF-β1、BMP-3的表达，促使退变的椎间盘修复，且呈剂量依赖性。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of Bugan Jianyao Decoction on the expression of basic fibroblast growth factor (bFGF), transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein-3 (BMP-3) in rat model of lumbar disc degeneration. Methods: A total of 90 SD rats were randomly divided into normal control group, sham operation group, model group, low, medium and high dose Bugan Jianyao Decoction groups, with 15 rats in each group. The normal control group was not treated. The sham operation group only exposed the intervertebral disc without intervertebral disc puncture. The remaining four groups prepared rat lumbar intervertebral disc degeneration model. After successful modeling, the low, medium and high doses of Bugan Jianyao Decoction groups were given low, medium and high doses of Bugan Jianyao Decoction by gavage. The normal control group, sham operation group and model group were given the same amount of normal saline. The contents of TGF-β1, bFGF mRNA and BMP-3 in intervertebral disc were detected before intervention, 20 d, 40 d after intervention. Results: Compared with the normal control group and the sham operation group, the expression of TGF-β1, bFGF mRNA and BMP-3 in the model group and each dose group of Bugan Jianyao Decoction increased before intervention(P<0.05). After 20 d and 40 d of intervention, the expression of TGF-β1, bFGF mRNA and BMP-3 in each dose group of Bugan Jianyao Decoction increased(P<0.05). Compared with the model group, there was no significant change in the content of TGF-β1, bFGF mRNA and BMP-3 in each dose group of Bugan Jianyao Decoction before intervention (P>0.05). After 20 d of intervention, the expression of TGF-β1, bFGF mRNA and BMP-3 in each dose group of Bugan Jianyao Decoction decreased (P<0.05). After 40 d of intervention, the expression of TGF-β1, bFGF mRNA and BMP-3 in the high dose group and the middle dose group of Bugan Jianyao Decoction decreased more significantly(P<0.05). The expression of TGF-β1, bFGF mRNA and BMP-3 in the low-dose group also decreased(P<0.05). After 40 d of intervention,the expression of TGF-β1, bFGF mRNA and BMP-3 in the high-dose group and the middledose group was significantly lower than that in the low-dose group(P<0.05). Conclusion: Bugan Jianyao Decoction can reduce the expression of bFGF, TGF-β1 and BMP-3 in the model of lumbar intervertebral disc degeneration in rats, and promote the repair of degenerative intervertebral disc in a dose-dependent manner. |
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