| 陈施翰,李 昆,楚理家,张春旭,王全晖.肝细胞癌组织中OATP1B3的表达及其作用的初步研究[J].现代生物医学进展英文版,2023,(22):4223-4229. |
| 肝细胞癌组织中OATP1B3的表达及其作用的初步研究 |
| Preliminary Study on the Expression and Role of OATP1B3 in Hepatocellular Carcinoma Tissue |
| Received:June 06, 2023 Revised:June 30, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.22.005 |
| 中文关键词: OATP1B3 肝细胞癌 临床病理特征 增殖 凋亡 |
| 英文关键词: OATP1B3 Hepatocellular carcinoma Clinicopathological characteristics Proliferation Apoptosis |
| 基金项目:河南省医学科技攻关计划科研项目（LHGJ20210807） |
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| 中文摘要: |
| 摘要 目的：探讨有机阴离子转运多肽1B3（OATP1B3）在肝细胞癌（肝癌）组织中的表达及作用。方法：通过免疫组化实验和免疫印迹试验（Western blot）检测肝癌组织及其癌旁组织中OATP1B3情况，并分析其与患者临床病理特征的相关性。采用实时荧光定量聚合酶链反应（qRT-PCR）检测OATP1B3在多株肝癌细胞中的表达情况，选择表达量相对较低的人肝癌细胞（HepG2和Huh7）细胞进行过表达实验，细胞毒实验（MTT）和流式细胞术分别检测OATP1B3对细胞增殖及凋亡的影响。结果：肝癌组织中OATP1B3表达水平明显低于癌旁组织（P<0.05），且与患者恶性肿瘤国际临床病期分类（TNM分期）、肿瘤分化程度、有无肿瘤复发显著相关（P<0.05）。过表达OATP1B3可抑制HepG2和Huh7细胞增殖，促进细胞凋亡。结论：OATP1B3在肝癌组织中低表达，上调其表达可抑制肝癌细胞增殖和促进细胞凋亡。OATP1B3可能是肝癌的抑癌基因，对肝癌的发生、发展具有重要作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression and role of organic anion transporting polypeptide 1B3 (OATP1B3) in hepatocellular carcinoma tissue. Methods: OATP1B3 in hepatocellular carcinoma tissues and adjacent tissues was detected by immunohistochemistry and Western blot, and its correlations with clinicopathological characteristics of patients was analyzed. The expression of OATP1B3 in multiple hepatoma cell lines was detected by quantitative real-time polymerase chain reaction(qRT-PCR), human hepatoma cells (HepG2 and Huh7) cells with relatively low expression were selected for overexpression experiments, and the effects of OATP1B3 on cell proliferation and apoptosis were investigated by cytotoxicity assay(MTT) and flow cytometry. Results: The expression level of OATP1B3 in hepatocellular carcinoma tissues was significantly lower than that in adjacent tissues, and was significantly correlated to International Classification of Clinical Stages for Patients with Malignant Tumors(TNM stage), tumor differentiation and tumour recurrence. Overexpression of OATP1B3 significantly inhibited the proliferation of HepG2 and Huh7 cells and promoted apoptosis. Conclusion: OATP1B3 is low expressed in hepatocellular carcinoma tissues, and its up regulation can inhibit the proliferation of hepatocellular carcinoma cells and promote cell apoptosis. OATP1B3 may be a tumor suppressor gene, which plays an important role in the occurrence and development of hepatocellular carcinoma. |
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