| 王 喻,庞兴华,田一鸣,路 灿,苑 著.基于核因子相关因子2/血红素加氧酶-1信号通路探讨异荭草素对乳腺癌细胞恶性生物学行为的影响[J].现代生物医学进展英文版,2023,(16):3021-3026. |
| 基于核因子相关因子2/血红素加氧酶-1信号通路探讨异荭草素对乳腺癌细胞恶性生物学行为的影响 |
| Effect of Isoorientin on Malignant Biological Behavior of Breast Cancer Cells Based on Nuclear Factor-related Factor 2/heme Oxygenase-1 Signaling Pathway |
| Received:March 05, 2023 Revised:March 27, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.16.004 |
| 中文关键词: 异荭草素 核因子相关因子2/血红素加氧酶-1通路 增殖 迁移 侵袭 乳腺癌 |
| 英文关键词: Isoorientin Nuclear factor-related factor 2/heme oxygenase-1 pathway Proliferation Migration Invasion Breast cancer |
| 基金项目:北京市属医院科研培育计划项目（PX20190102） |
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| 中文摘要: |
| 摘要 目的：探讨异荭草素（ISO）对乳腺癌（BC）细胞恶性生物学行为及核因子相关因子2（Nrf2）/血红素加氧酶-1（HO-1）信号通路的影响。方法：体外培养人BC细胞系MDA-MB-231并分组：MDA-MB-231组、MDA-MB-231+ISO组（100 μmol/L ISO处理）、MDA-MB-231+ISO+OE-NC组（转染OE-NC后用100 μmol/L ISO处理）、MDA-MB-231+ISO+OE-Nrf2组（转染OE-Nrf2后用100 μmol/L ISO处理）。采用细胞计数试剂盒-8（CCK-8）检测MDA-MB-231细胞增殖；流式细胞术检测MDA-MB-231细胞周期和凋亡；Transwell实验检测MDA-MB-231细胞的侵袭和迁移能力；Western blot检测Nrf2/HO-1信号通路相关蛋白及凋亡蛋白表达。结果：与MDA-MB-231组相比，MDA-MB-231+ISO组、MDA-MB-231+ISO+OE-NC组细胞活力、S期和G2期细胞比例、迁移和侵袭能力、B淋巴细胞瘤-2（Bcl-2）、Nrf2、HO-1、基质金属蛋白酶-9（MMP-9）蛋白水平显著下降（P<0.05），细胞凋亡率、G1/G0期细胞比例以及Bax、cleaved-Caspase-3蛋白水平显著上升（P<0.05）。与MDA-MB-231+ISO组相比，MDA-MB-231+ISO+OE-Nrf2组细胞活力、S期和G2期细胞比例、迁移和侵袭能力、Bcl-2、Nrf2、HO-1、MMP-9蛋白水平显著上升（P<0.05），细胞凋亡率、G1/G0期细胞比例以及Bax、cleaved-Caspase-3蛋白水平显著降低（P<0.05）。结论：ISO可能通过抑制Nrf2/HO-1信号通路，抑制MDA-MB-231细胞恶性增殖、迁移和侵袭等行为。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the influences of isoorientin(ISO) on malignant biological ehavior of breast cancer (BC) cells and nuclear factor-related factor 2(Nrf2)/heme oxygenase-1(HO-1) signaling pathway. Methods: Human BC cell line MDA-MB-231 was cultured in vitro and separated into groups: MDA-MB-231 group, MDA-MB-231+ISO group(100 μmol/L ISO treatment), MDA-MB-231+ ISO+OE-NC group (treated with 100 μmol/L ISO after transfection of OE-NC), and MDA-MB-231+ISO+OE-Nrf2 group (treated with 100 μmol/L ISO after transfection of OE-Nrf2). MDA-MB-231 cells proliferation was detected by cell count kit-8 (CCK-8). Flow cytometry was used to detect MDA-MB-231 cells cycle and apoptosis. Transwell assay was used to detect the invasion and migration abilities of MDA-MB-231 cells. Western blot was used to detect the expression of Nrf2/HO-1 signaling pathway-related proteins and apoptosis proteins. Results: Compared with MDA-MB-231 group, MDA-MB-231+ISO group and MDA-MB-231+ ISO+OE-NC group had obviously lower cell viability, S phase and G2 phase cells ratios, migration and invasion abilities, B-lymphocytoma-2 (Bcl-2), Nrf2, HO-1, matrix metalloproteinase-9 (MMP-9) protein levels (P<0.05), and obviously higher apoptosis rate, G1/G0 phase cells ratios, and Bax and cleaved-Caspase-3 protein levels(P<0.05). Compared with MDA-MB-231+ISO group, MDA-MB-231+ISO+OE-Nrf2 group had obviously higher cell viability, S phase and G2 phase cells ratios, migration and invasion abilities, Bcl-2, Nrf2, HO-1, MMP-9 protein levels(P<0.05), and obviously lower apoptosis rate, G1/G0 phase cells ratios, and Bax and cleaved-Caspase-3 protein levels(P<0.05). Conclusion: ISO may inhibit the malignant proliferation, migration and invasion of MDA-MB-231 cells by inhibiting the Nrf2/HO-1 signaling pathway. |
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