| 王思成,陈浩龙,冯 超,邓 飞,贾 斌.肌层浸润性膀胱癌组织KMT2D、MCM6与临床病理特征和预后的关系[J].现代生物医学进展英文版,2023,(14):2786-2790. |
| 肌层浸润性膀胱癌组织KMT2D、MCM6与临床病理特征和预后的关系 |
| Relationship between KMT2D, MCM6 and Clinicopathologic Features and Prognosis in the Tissue of Muscular Invasive Bladder Cancer |
| Received:February 06, 2023 Revised:February 26, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.14.036 |
| 中文关键词: 肌层浸润性膀胱癌 KMT2D MCM6 临床病理特征 预后 |
| 英文关键词: Muscular invasive bladder cancer KMT2D MCM6 Clinicopathologic features Prognosis |
| 基金项目:山西省重点研发计划项目（201903D321032） |
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| 中文摘要: |
| 摘要 目的：探讨肌层浸润性膀胱癌（MIBC）组织赖氨酸甲基转移酶2D（KMT2D）、微小染色体维持蛋白6（MCM6）与临床病理特征和预后的关系。方法：选择2017年5月至2019年10月长治医学院附属和平医院行手术治疗的MIBC患者96例，应用逆转录-实时定量聚合酶链式反应（qRT-PCR）检测癌组织及癌旁正常组织KMT2D 信使核糖核酸（mRNA）、MCM6 mRNA表达，分析KMT2D mRNA、MCM6 mRNA表达与MIBC患者临床病理特征的关系，应用Pearson相关分析法分析MIBC患者癌组织KMT2D mRNA及MCM6 mRNA表达的相关性。随访3年，比较死亡MIBC患者与存活MIBC患者癌组织KMT2D mRNA、MCM6 mRNA表达，并应用Kaplan-Meier生存曲线分析不同KMT2D mRNA、MCM6 mRNA表达分组MIBC患者预后情况。结果：MIBC癌组织中KMT2D mRNA表达水平显著低于癌旁组织，MCM6 mRNA表达水平显著高于癌旁组织（P<0.05）。MIBC患者癌组织中KMT2D mRNA、MCM6 mRNA表达与侵犯输尿管、淋巴结转移、TNM分期显著相关（P<0.05），且两者表达呈负相关（P<0.05）。至随访截止，MIBC患者死亡45例，死亡组患者癌组织中KMT2D mRNA表达水平显著低于存活组、MCM6 mRNA表达水平显著高于存活组（P<0.05）。生存曲线结果显示KMT2D mRNA高表达组3年生存率显著高于KMT2D mRNA低表达组；MCM6 mRNA低表达组3年生存率显著高于MCM6 mRNA高表达组（P<0.05）。结论：MIBC癌组织中KMT2D低表达、MCM6高表达，与MIBC侵犯输尿管、淋巴结转移、TNM分期及患者预后不良有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the relationship between lysine (K)-specific methyltransferase 2D (KMT2D) , minichromosome maintenance protein 6 (MCM6) and clinicopathologic features and prognosis in the tissue of muscular invasive bladder cancer (MIBC). Methods: 96 patients with MIBC who underwent surgical treatment in Heping Hospital Affiliated to Changzhi Medical College from May 2017 to October 2019 were selected. The expression of KMT2D messenger RNA (mRNA) and MCM6 mRNA in cancer tissue and normal paracancer tissue were detected by reverse transcription-real-time quantitative polymerase chain reaction (qRT-PCR). The relationship between the expressions of KMT2D mRNA and MCM6 mRNA and the clinicopathological features of patients with MIBC were analyzed. Pearson correlation analysis was used to analyze the correlation between the expressions of KMT2D mRNA and MCM6 mRNA in cancer tissue of patients with MIBC. After a 3-year follow-up, the expressions of KMT2D mRNA and MCM6 mRNA in cancer tissue of dead patients and surviving patients with MIBC were compared, and Kaplan-Meier survival curve was used to analyze the prognosis of patients with MIBC and with different expressions of KMT2D mRNA and MCM6 mRNA. Results: The expression level of KMT2D mRNA in the MIBC cancer tissue was significantly lower than that in the paracancer tissue, and the expression level of MCM6 mRNA was significantly higher than that in the paracancer tissue (P<0.05). The expressions of KMT2D mRNA and MCM6 mRNA in the cancer tissue of patients with MIBC were significantly correlated with invasion of ureter, lymph node metastasis and TNM stage(P<0.05), and the expressions of both were negatively correlated(P<0.05). By the end of follow-up, 45 patients had died. The expression level of KMT2D mRNA in the cancer tissue of the death group was significantly lower than that of the survival group, and the expression level of MCM6 mRNA was significantly higher than that of the survival group (P<0.05). The results of survival curve showed that the 3-year survival rate of the KMT2D mRNA high expression group was significantly higher than that of the KMT2D mRNA low expression group. The 3-year survival rate of the MCM6 mRNA low expression group was significantly higher than that of the MCM6 mRNA high expression group(P<0.05). Conclusion: The low expression of KMT2D and high expression of MCM6 in MIBC cancer tissue are related to MIBC invasion of ureter, lymph node metastasis, TNM stage and poor prognosis of patients. |
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