| 侯静雯,张玉婷,朱新华,张 蕾,张晓阳.TRB3基因敲除减轻糖尿病小鼠中骨骼肌萎缩和纤维化的研究[J].现代生物医学进展英文版,2023,(14):2620-2624. |
| TRB3基因敲除减轻糖尿病小鼠中骨骼肌萎缩和纤维化的研究 |
| TRB3 Gene Knockout Alleviates Skeletal Muscle Atrophy and Fibrosis in Diabetic Mice |
| Received:February 06, 2023 Revised:February 28, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.14.004 |
| 中文关键词: TRB3基因 糖尿病小鼠 骨骼肌萎缩 纤维化 |
| 英文关键词: TRB3 gene Diabetic mice Skeletal muscle atrophy Fibrosis |
| 基金项目:新疆维吾尔自治区自然科学基金项目（2021D01C451） |
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| 中文摘要: |
| 摘要 目的：探讨TRB3基因敲除对糖尿病小鼠中骨骼肌萎缩和纤维化的影响。方法：选择30只TRB3敲除（TRB3-/-）小鼠和30只C57/BL6J小鼠，随机分为4组，包括TRB3基因敲除糖尿病模型组（A组）、TRB3基因敲除正常对照组（B组）、普通对照组（C组）和糖尿病模型组（D组）。正常对照组小鼠喂养标准大鼠饲料，糖尿病模型小鼠使用腹腔注射小剂量链脲佐菌素的方法建立糖尿病小鼠模型。进行前肢握力测试、悬栅试验、肌肉功能试验，同时测定骨微结构各指标水平、抗I型胶原蛋白和抗III型胶原蛋白水平、萎缩基因MuRF1和Atrogin-1水平。结果：A组、B组、D组小鼠前肢握力显著低于C组，其中D组小鼠前肢握力最低（P<0.05）；A组、B组、D组小鼠悬栅试验倒挂时间显著低于C组，其中D组小鼠悬栅试验倒挂时间最短（P<0.05）；A组、B组、D组小鼠肌肉功能CSA值显著低于C组，其中D组小鼠肌肉功能CSA值最低（P<0.05）；A组、B组、D组小鼠骨微结构指标BMD、BV/TV、Tb.N、Tb.Th值显著小于C组，其中D组数值最低（P<0.05）；而Tb.Sp和SMI值显著大于C组，其中D组数值最高（P<0.05）；A组、B组、D组小鼠抗I型胶原蛋白和抗III型胶原蛋白水平显著高于C组，其中D组抗I型胶原蛋白和抗III型胶原蛋白水平最高（P<0.05）；A组、B组、D组小鼠萎缩基因MuRF1和Atrogin-1水平显著高于C组，其中D组萎缩基因MuRF1和Atrogin-1水平最高（P<0.05）。结论：TRB3基因敲除可显著减轻糖尿病小鼠中骨骼肌萎缩和纤维化，增加骨强度。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of TRB3 gene knockout on skeletal muscle atrophy and fibrosis in diabetic mice. Methods: Thirty TRB3 knockout (TRB3-/-) mice and 30 C57/BL6J mice were randomly divided into 4 groups, including TRB3 knockout diabetes model group (group A), normal TRB3 knockout control group (group B), normal control group (group C) and diabetes model group (group D). Normal control group mice were fed standard rat feed, and diabetic mice were injected intraperitoneally with low dose of streptozotocin to establish diabetic mice model. The forelimb grip strength test, suspension grid test and muscle function test were performed. Meanwhile, the levels of bone microstructure, anti-type I collagen and anti-type III collagen, atrophy gene MuRF1 and atrophy gene atrophy 1 were determined. Results: The hand grip strength of group A, B and D was significantly lower than that of group C, and the hand grip strength of group D was the lowest(P<0.05). The inverted hanging time of mice in groups A, B and D was significantly lower than that in group C, and the inverted hanging time of mice in group D was the shortest(P<0.05). CSA value of muscle function in groups A, B and D was significantly lower than that in group C, and CSA value of muscle function in group D was the lowest(P<0.05). The bone microstructure indexes BMD, BV/TV, Tb.N and Tb.Th in groups A, B and D were significantly lower than those in group C, and the lowest values were in group D(P<0.05). The values of Tb.Sp and SMI in group C were significantly higher than those in group C, and the values in group D were the highest(P<0.05). The levels of anti-type I collagen and anti-type III collagen in groups A, B and D were significantly higher than those in group C, and the levels of anti-type I collagen and anti-type III collagen in group D were the highest (P<0.05). The levels of atrophy gene MuRF1 and Atrogin-1 in groups A, B and D were significantly higher than those in group C, and the levels of MuRF1 and Atrogin-1 in group D were the highest(P<0.05). Conclusion: TRB3 gene knockout significantly reduced skeletal muscle atrophy and fibrosis and increased bone strength in diabetic mice. |
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