| 吴国玉,杨 俊,熊绍权,祝 捷,李建梅.人参皂苷Rh2对大鼠C6胶质瘤细胞Siah-1、Synaptophysin、MMP9及VEGF表达的影响[J].现代生物医学进展英文版,2023,(13):2419-2423. |
| 人参皂苷Rh2对大鼠C6胶质瘤细胞Siah-1、Synaptophysin、MMP9及VEGF表达的影响 |
| Impacts of Ginsenoside Rh2 on the Siah-1, Synaptophysin, MMP9 and VEGF Expression in Rat C6 Glioma Cells |
| Received:February 04, 2023 Revised:February 27, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.13.004 |
| 中文关键词: 人参皂苷Rh2 胶质瘤 Siah-1 Synaptophysin MMP9 VEGF 增殖 侵袭 凋亡 |
| 英文关键词: Ginsenoside Rh2 Glioma Siah-1 Synaptophysin MMP9 VEGF Proliferation Invasion Apoptosis |
| 基金项目:四川省中医药管理局科学技术研究专项课题（2021MS445） |
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| 中文摘要: |
| 摘要 目的：探讨人参皂苷Rh2对大鼠C6胶质瘤细胞Siah-1、突触素（Synaptophysin）、基质金属蛋白酶-9（MMP-9）血管内皮生长因子（VEGF）表达的影响。方法：将大鼠C6胶质瘤细胞分为对照组、人参皂苷Rh2低剂量组（16 μg/mL）、人参皂苷Rh2中剂量组（32 μg/mL）、人参皂苷Rh2高剂量组（48 μg/mL），CCK-8法和平板克隆实验检测细胞增殖；流式细胞术检测细胞凋亡；Transwell检测细胞侵袭；实时荧光定量-聚合酶链式反应（qRT-PCR）检测C6胶质瘤细胞中VEGF、Siah-1、Synaptophysin、MMP-9 mRNA表达；蛋白质印迹法（Western blot）检测C6胶质瘤细胞中VEGF、Siah-1、Synaptophysin、MMP-9蛋白表达。结果：与对照组比较，人参皂苷Rh2低剂量组、人参皂苷Rh2中剂量组、人参皂苷Rh2高剂量组大鼠C6胶质瘤细胞OD450值（24 h、48 h）、克隆形成率、细胞侵袭数、VEGF、Synaptophysin、MMP-9 mRNA及蛋白表达降低，细胞凋亡率、Siah-1 mRNA及蛋白表达升高，且呈剂量依赖性（P<0.05）。结论：人参皂苷Rh2可能通过上调Siah-1，下调VEGF、Synaptophysin、MMP-9表达来抑制大鼠C6胶质瘤细胞增殖与侵袭，促进细胞凋亡。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the impacts of ginsenoside Rh2 on the Siah-1, synaptophysin, matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) expression in rat C6 glioma cells. Methods: Rat C6 glioma cells were grouped into control group, low-dose ginsenoside Rh2 group (16 μg/mL), medium dose ginsenoside Rh2 group (32 μg/mL), and high-dose ginsenoside Rh2 group (48 μg/mL), cell proliferation was detected by CCK-8 method and plate cloning test. Cell apoptosis was detected by flow cytometry. Cell invasion was detected by Transwell. The VEGF, Siah-1, Synaptophysin and MMP-9 mRNA expression in C6 glioma cells was detected by real-time fluorescence quantitation-polymerase chain reaction (qRT-PCR); and Western blot was used to detect the protein VEGF, Siah-1, Synaptophysin and MMP-9 expression in C6 glioma cells. Results: Compared with the control group, the OD450 value (24 h, 48 h), clone formation rate, cell invasion number, VEGF, Synaptophysin, MMP-9 mRNA and protein expression in C6 glioma cells in the low-dose ginsenoside Rh2 group, the medium dose ginsenoside Rh2 group, and the high-dose ginsenoside Rh2 group were decreased, the cell apoptosis rate and the Siah-1 mRNA and protein expression were increased, which were dose dependent (P<0.05). Conclusion: Ginsenoside Rh2 may inhibit the proliferation and invasion of rat C6 glioma cells and promote cell apoptosis by up-regulating Siah-1 and down-regulating the VEGF, Synaptophysin and MMP-9 expression. |
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