| 王晓丽,王 燕,宋诸臣,肖金章,宋佳烨,曹永峰.外泌体lncRNA-MIR100HG/miR-100表达变化与胃癌临床病理特征、无疾病进展生存率的相关性[J].现代生物医学进展英文版,2023,(12):2305-2309. |
| 外泌体lncRNA-MIR100HG/miR-100表达变化与胃癌临床病理特征、无疾病进展生存率的相关性 |
| Correlation between the Expression of LncRNA-MIR100HG/miR-100 in Exosomes and Clinicopathological Characteristics and Progression Free Survival Rate of Gastric Cancer |
| Received:December 06, 2022 Revised:December 30, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.12.019 |
| 中文关键词: 胃癌 外泌体 lncRNA-MIR100HG miR-100 病理特征 无进展生存 预后 |
| 英文关键词: Gastric cancer Exosomes LncRNA-MIR100HG MiR-100 Pathological characteristics Survival without progression Prognosis |
| 基金项目:江苏省基础研究计划(自然科学基金)——面上项目（BK20 19 1208）；南通市卫生健康委员会科研课题（MA2020008） |
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| 中文摘要: |
| 摘要 目的：探究胃癌患者血清外泌体长链非编码RNA-MIR100HG（lncRNA-MIR100HG）及微小核糖核酸-100（miR-100）表达情况，并分析其与患者临床病理特征和无疾病进展生存率之间的相关性。方法：收集60例胃癌患者和60例良性疾病患者，提取外泌体，检测lncRNA-MIR100HG/miR-100的表达情况并进行组间比较。采用Pearson相关分析lncRNA-MIR100HG与miR-100表达水平的相关性，应用单因素卡方检验分析与胃癌患者临床病理特征相关性，采用Kaplan-Meier生存分析lncRNA-MIR100HG/miR-100表达情况与无疾病进展生存率。结果：（1）胃癌组患者血清lncRNA-MIR100HG显著高于，miR-100相对表达水平显著低于胃良性疾病组（P<0.05）；（2）Pearson相关分析结果显示血清lncRNA-MIR100HG和miR-100存在显著负相关关系（r=-0.483，P<0.05）；（3）单因素卡方检验分析结果显示：胃癌患者血清lncRNA-MIR100HG相对表达水平与肿瘤大小、分化程度、肿瘤浸润深度、临床分期、淋巴结转移和远处转移相关，血清miR-100相对表达水平与肿瘤大小、分化程度、临床分期和淋巴结转移相关（P<0.05）；（4）血清lncRNA-MIR100HG、miR-100低水平表达胃癌患者PFS显著长于高水平患者（χ2=37.371，P＜0.05），miR-100高水平表达胃癌患者PFS显著长于低水平患者（χ2=28.631，P＜0.05）。结论：胃癌患者血清外泌体lncRNA-MIR100HG/miR-100表达水平与肿瘤大小、肿瘤浸润深度、临床分期等病理特征相关，lncRNA-MIR100HG高表达与miR-100低表达可能提示胃癌患者预后越差。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of long chain non coding RNA-MIR100HG (lncRNA-MIR100HG) and microribonucleic acid-100 (miR-100) in serum exosomes of patients with gastric cancer, and analyze the correlation between them and clinical pathological characteristics and progression free survival rate. Methods: Blood samples from 60 patients with gastric cancer and 60 patients with benign diseases were collected, and the exosomes were extracted. The expression of lncRNA-MIR100HG/miR-100 was detected and compared between groups. Pearson correlation was used to analyze the correlation between the expression level of lncRNA-MIR100HG and miR-100, single factor chi square test was used to analyze the correlation with the clinicopathological characteristics of gastric cancer patients, and Kaplan-Meier survival was used to analyze the expression of lncRNA-MIR100HG/miR-100 and the progression free survival rate. Results: (1) The level of serum lncRNA-MIR100HG in patients with gastric cancer was higher than that in patients with gastric cancer, and the relative expression level of miR-100 was lower than that in patients with benign gastric diseases (P<0.05); (2) Pearson correlation analysis showed that there was a negative correlation between serum lncRNA-MIR100HG and miR-100 (r=-0.483, P<0.05); (3) Single factor chi square analysis showed that the relative expression level of serum lncRNA-MIR100HG in gastric cancer patients was correlated with tumor size, differentiation, depth of tumor invasion, clinical stage, lymph node metastasis and distant metastasis, and the relative expression level of serum miR-100 was correlated with tumor size, differentiation, clinical stage and lymph node metastasis (P<0.05); (4) PFS of gastric cancer patients with low level expression of serum lncRNA-MIR100HG and miR-100 was longer than that of patients with high level expression (χ2=37.371, P<0.05), PFS of gastric cancer patients with high miR-100 expression was longer than that of patients with low miR-100 expression (χ2=28.631, P＜0.05). Conclusion: The expression level of serum exosomes lncRNA-MIR100HG/miR-100 in patients with gastric cancer is increased, which is related to tumor size, tumor invasion depth, clinical stage and other pathological characteristics. High expression of lncRNA-MIR100HG and low expression of miR-100 may indicate that the prognosis of gastric cancer patients is worse. |
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