| 杨丽琴,丛媛媛,胡洪源,孙继超,雍亚云,吴玉苗,李伟伟.三十六荡坎蛤散干预哮喘缓解期小鼠肠组织蛋白质组学差异表达研究[J].现代生物医学进展英文版,2023,(12):2215-2220. |
| 三十六荡坎蛤散干预哮喘缓解期小鼠肠组织蛋白质组学差异表达研究 |
| Study on Sanshiliudang Kange Powder Intervening Proteomic Differential Expression in Intestinal Tissue of Asthma in Remission Mice |
| Received:December 06, 2022 Revised:December 28, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.12.003 |
| 中文关键词: 三十六荡坎蛤散 哮喘缓解期 肺-肠轴 蛋白质组学 |
| 英文关键词: Sanshiliudang Kange Powder Asthma in remission Lung-intestinal axis Proteomics |
| 基金项目:国家自然科学基金项目（81860867）；中医药人才队伍建设专项经费-广西名中医工作室建设项目（2021022-001-08）；广西中医药大学2021年校级博士研究生科研创新项目（YCBXJ2021017） |
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| 中文摘要: |
| 摘要 目的：用蛋白质组学方法探究三十六荡坎蛤散对哮喘缓解期小鼠肠组织蛋白差异表达的影响。方法：将45只SPF级雌性C57BL/6小鼠随机分为正常组、模型组、三十六荡坎蛤散组，采用HE染色法观察小鼠肺组织的病理变化，采用ELISA法检测肺泡灌洗液（BALF）中白介素-1β（IL-1β）、白介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平，提取肠组织蛋白，利用非标记（Label-free）定量蛋白质组学技术研究蛋白表达差异。结果：与模型组比较，三十六荡坎蛤散组显著改善了哮喘缓解期小鼠肺组织的病理状态，减轻炎症反应，改善小鼠哮喘症状；BALF中IL-1β，IL-6和TNF-α水平降低（P<0.01）；蛋白质组学研究共鉴定到6634个蛋白，模型组和三十六荡坎蛤散组共有232个差异蛋白，模型组和正常组共有34个差异蛋白，模型组与正常组比较有差异而三十六荡坎蛤散使差异趋势逆转的蛋白有6个，即Hsd17b2，Plp2，Vnn1，Olfm4，Hsbp1，Gimd。GO分析显示差异表达蛋白主要参与新陈代谢、炎症反应、神经系统调节等生物过程，KEGG分析显示，三十六荡坎蛤散主要涉及新陈代谢、炎症反应和免疫调节等10条信号通路。结论：三十六荡坎蛤散可能通过逆转Hsd17b2，Plp2，Vnn1，Olfm4，Hsbp1，Gimd的表达，参与多个生物过程，作用多条信号通路实现干预哮喘。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of Sanshiliudang Kange Powder(SKP) on the proteomic differential expression in the intestinal tissue of mice with bronchial asthma during remission by proteomics technology. Methods: Forty-five SPF female C57BL/6 mice were randomly divided into normal group, model group, and Sanshiliudang Kange Powder group. HE staining was used to observe the pathological changes in mice lung tissue. The levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the bronchoalveolar lavage fluid (BALF) were determined by ELISA. Proteins were extracted from the intestinal tissues, and the protein expression differences were studied by Label-free quantitative proteomics. Results: Compared with that in the model group, Sanshiliudang Kange Powder significantly improved the pathological state of lung tissue, alleviated inflammation and improved asthma symptoms in mice with asthma during remission. Levels of IL-1β, IL-6 and TNF-α decreased in BALF (P<0.05); A total of 6634 proteins were identified in proteomics research, there were 232 different proteins in the model group and the Sanshiliudang Kange Powder group, and there were 34 different proteins in the model group and the normal group. There were differences between the model group and the normal group, and Sanshiliudang Kange Powder could reverse the trend of the difference. There were six different proteins namely Hsd17b2, Plp2, Vnn1, Olfm4, Hsbp1, Gimd. GO analysis showed that differentially expressed proteins were mainly involved in biological processes such as metabolism, inflammatory response, nervous system regulation. KEGG analysis showed that Sanshiliudang Kange Powder was mainly involved in 10 signaling pathways including metabolism, inflammatory response and immune regulation. Conclusion: Sanshiliudang Kange Powder may achieve the intervention of asthma by reversing the expression of Hsd17b2, Plp2, Vnn1, Olfm4, Hsbp1and Gimd, participating in multiple biological processes and acting on multiple signaling pathways. |
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