| 姚 彦,郭春霞,郭 莉,胡向敏,张淑静.北沙参对肺癌大鼠PI3K/Akt信号通路及免疫炎症反应的影响[J].现代生物医学进展英文版,2023,(11):2035-2039. |
| 北沙参对肺癌大鼠PI3K/Akt信号通路及免疫炎症反应的影响 |
| Effects of Radix Glehniae on PI3K/Akt Signal Pathway and Immune Inflammatory Response in Lung Cancer Rats |
| Received:February 06, 2023 Revised:February 28, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.11.007 |
| 中文关键词: 肺癌 大鼠 北沙参 PI3K/Akt信号通路 免疫炎症反应 |
| 英文关键词: Lung cancer Rats Radix Glehniae PI3K/Akt signal path Immune inflammatory reaction |
| 基金项目:陕西省科技计划项目（2019SF-040) |
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| 中文摘要: |
| 摘要 目的：探讨北沙参对肺癌大鼠PI3K/Akt信号通路及免疫炎症反应的影响。方法：从60只Wistar大鼠中随机选取15只作为对照组，其余45只大鼠采用气管内灌注致癌碘油液建立肺癌模型，按照随机数字表法将其分为模型组（n=15）、环磷酰胺组（n=15）、北沙参组（n=15），开展前瞻性研究。对照组、模型组给予生理盐水灌胃，环磷酰胺组给予环磷酰胺干预，北沙参组给予北沙参干预。比较各组大鼠免疫功能、炎症因子水平及PI3K/Akt表达。结果：与对照组相比，环磷酰胺组、北沙参组、模型组脾脏指数依次降低，而肺脏指数依次升高（P<0.05）。与对照组相比，环磷酰胺组、北沙参组、模型组CD3+、CD4+百分比及CD4+/CD8+依次降低，而CD8+百分比依次升高（P<0.05）。与对照组相比，环磷酰胺组、北沙参组、模型组白介素6（IL-6）、白介素1β（IL-1β）、肿瘤坏死因子-α（TNF-α）水平依次升高（P<0.05）。与对照组相比，环磷酰胺组、北沙参组、模型组PI3K、Akt表达水平依次升高（P<0.05）。结论：北沙参能够有效调节肺癌大鼠的脏器指数，促进免疫功能改善，抑制炎症反应，调控PI3K/Akt信号通路可能是其发挥作用的重要机制。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of Radix Glehniae on PI3K/Akt signal pathway and immune inflammatory reaction in lung cancer rats. Methods: Fifteen Wistar rats were randomly selected from 60 Wistar rats as the control group, and the remaining 45 rats were perfused with carcinogenic iodine oil into the trachea to establish a lung cancer model. They were divided into model group (n=15), cyclophosphamide group(n=15), and Radix Glehniae group(n=15) according to the random number table method, and carried out a prospective study. The control group and the model group were given normal saline by gavage, the cyclophosphamide group was given cyclophosphamide intervention, and the Northern Salvia miltiorrhiza group was given Northern Salvia miltiorrhiza intervention. The immune function, inflammatory factor level and PI3K/Akt expression of rats in each group were compared. Results: Compared with the control group, the spleen index of the cyclophosphamide group, the Radix Glehniae group and the model group decreased in turn, while the lung index increased in turn(P<0.05). Compared with the control group, the percentage of CD3+, CD4+ and CD4+/CD8+ in cyclophosphamide group, Radix Glehniae group and model group decreased in turn, while the percentage of CD8+ increased in turn (P<0.05). Compared with the control group, the cyclophosphamide group, the northern salvia miltiorrhiza group, and the model group had interleukin 6 (IL-6) and interleukin 1 β (IL-1β), Tumor necrosis factor-α (TNF-α) The levels increased in turn (P<0.05). Compared with the control group, the expression levels of PI3K and Akt in cyclophosphamide group, Radix Glehniae group and model group increased in turn (P<0.05). Conclusion: It may be an important mechanism of its effect that Radix Salviae Miltiorrhizae can effectively regulate the organ index of lung cancer rats, promote the improvement of immune function, inhibit inflammatory reaction, and regulate the PI3K/Akt signal pathway. |
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