| 马嘉嘉,郑 倩,李大伟,祁晶晶,薛 冰.小檗碱对幽门螺旋杆菌感染小鼠胃粘膜损伤和免疫功能的影响机制研究[J].现代生物医学进展英文版,2023,(11):2019-2024. |
| 小檗碱对幽门螺旋杆菌感染小鼠胃粘膜损伤和免疫功能的影响机制研究 |
| Effect of Berberine on Gastric Gucosal Injury and Immune Function in Mice Infected with Helicobacter Pylori |
| Received:January 12, 2023 Revised:January 31, 2023 |
| DOI：10.13241/j.cnki.pmb.2023.11.004 |
| 中文关键词: 小檗碱 幽门螺旋杆菌 胃粘膜损伤 免疫功能 影响机制 |
| 英文关键词: Berberine Helicobacter pylori Gastric mucosa injury Immune function Influence mechanism |
| 基金项目:国家自然科学基金项目（81860352） |
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| 中文摘要: |
| 摘要 目的：探讨小檗碱对幽门螺旋杆菌感染小鼠胃粘膜损伤和免疫功能的影响机制。方法：选择SPF雄性小鼠50只，随机分为5组，包括空白对照组、幽门螺杆菌模型组、小檗碱低剂量组（25 mg/mL，A组）、中剂量组（50 mg/mL，B组）和高剂量组（100 mg/kg/d，C组）。空白组大鼠腹腔注射等容量的生理盐水，其余组使用口服幽门螺杆菌的方法建立幽门螺旋杆菌感染小鼠模型。进行胃黏膜上皮细胞存活率和凋亡率检测，同时测定胃黏膜上皮细胞凋亡蛋白水平、炎症因子水平、免疫功能以及IL-4/STAT6 mRNA水平。结果：模型组、小檗碱低剂量组、中剂量组和高剂量组胃黏膜上皮细胞存活率均显著低于空白对照组（P<0.05）；且随着小檗碱剂量的增加，细胞存活率逐渐升高（P<0.05）；模型组、小檗碱低剂量组、中剂量组和高剂量组胃黏膜上皮细胞凋亡率均显著高于空白对照组（P<0.05）；且随着小檗碱剂量的增加，细胞凋亡率逐渐降低（P<0.05）；与模型组比较，小檗碱低、中、高剂量组小鼠胃黏膜上皮细胞凋亡蛋白Bax和Cl-caspase-3的水平、TNF-α、IFN-γ、IL-10和TGF-β的水平均显著降低（P<0.05），且随着小檗碱剂量的增加，Bax和Cl-caspase-3的水平、TNF-α、IFN-γ、IL-10和TGF-β的水平逐渐降低（P<0.05）；与模型组比较，小檗碱低、中、高剂量组上清液中CD3+、CD4+和CD8+的水平、IL-4 mRNA和STAT6 mRNA均显著升高（P<0.05），且随着小檗碱剂量的增加，CD3+、CD4+和CD8+水平、IL-4 mRNA和STAT6 mRNA逐渐升高（P<0.05）。结论：小檗碱能够减轻幽门螺旋杆菌感染小鼠胃粘膜损伤，提升免疫功能，可能与IL-4/STAT6信号通路有关。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of berberine on gastric mucosal injury and immune function in mice infected with Helicobacter pylori. Methods: Fifty SPF male mice were randomly divided into 5 groups, including blank control group, Helicobacter pylori model group, berberine low-dose group (25 mg/mL, group A), medium-dose group (50 mg/mL, group B) and high-dose group (100 mg/kg/d, group C). Rats in blank group were intraperitoneally injected with normal saline of equal volume, and the other groups were orally administered Helicobacter pylori to establish a mouse model of Helicobacter pylori infection. The survival rate and apoptosis rate of gastric mucosal epithelial cells were detected, and the levels of apoptotic protein, inflammatory factors, immune function and IL-4/STAT6 mRNA of gastric mucosal epithelial cells were measured. Results: The survival rate of gastric mucosal epithelial cells in model group, berberine low-dose, medium-dose and high-dose groups was significantly lower than that in blank control group (P<0.05). With the increase of berberine dose, cell survival rate was gradually increased(P<0.05). The apoptosis rate of gastric mucosal epithelial cells in model group, berberine low-dose, medium-dose and high-dose groups was significantly higher than that in blank control group (P<0.05). With the increase of berberine dose, the apoptosis rate decreased gradually (P<0.05). Compared with model group, the levels of apoptotic protein Bax and Cl-caspase-3, TNF-α, IFN-γ, IL-10 and TGF-β of mouse gastric mucosal epithelial cells in berberine low, medium and high dose groups were significantly decreased(P<0.05), and with the increase of berberine dose, The levels of Bax and Cl-caspase-3, TNF-α, IFN-γ, IL-10 and TGF-β were decreased(P<0.05). Compared with model group, the levels of CD3+, CD4+ and CD8+, IL-4 mRNA and STAT6 mRNA in the supernatant of berberine low-dose, medium-dose and high-dose groups were significantly increased(P<0.05), and with the increase of berberine dose, CD3+, CD4+ and CD8+ levels, IL-4 mRNA and STAT6 mRNA were increased gradually(P<0.05). Conclusion: Berberine can alleviate gastric mucosal damage and enhance immune function in mice infected with Helicobacter pylori, which may be related to IL-4/STAT6 signaling pathway. |
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