| 王 丽,张梦婷,陈 阳,魏 琳,王建锋.人羊膜上皮干细胞联合富血小板血浆对宫腔粘连大鼠纤维化因子、炎症因子的作用[J].现代生物医学进展英文版,2023,(8):1430-1435. |
| 人羊膜上皮干细胞联合富血小板血浆对宫腔粘连大鼠纤维化因子、炎症因子的作用 |
| Effect of Human Amniotic Epithelial Cells Combined with Platelet Rich Plasma on Fibrotic Factors and Inflammatory Factors in Rats with Intrauterine Adhesions |
| Received:October 14, 2022 Revised:November 10, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.08.006 |
| 中文关键词: 宫腔粘连 人羊膜上皮干细胞 富血小板血浆 纤维化 炎性反应 |
| 英文关键词: Intrauterine adhesion Human amniotic epithelial stem cells Platelet-rich plasma Fibrosis Inflammatory reaction |
| 基金项目:陕西省科技厅科研基金项目（2020JM-604） |
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| 中文摘要: |
| 摘要 目的：探究人羊膜上皮干细胞（hAECs）联合富血小板血浆（PRP）对宫腔粘连大鼠的治疗效果，为临床中优化宫腔粘连治疗方法理论基础。方法：选用SPF级雌性SD大鼠共40只，依照随机数字表法随机分为空白组（A组）、假手术组（B组）、hAECs组（C组）、PRP组（D组）、hAECs+PRP组（E组），每组各8只。A组不做任何处理，B组仅做麻醉、开关腹腔处理，C组、D组、E组使用搔刮法制备宫腔粘连模型。随后C组进行hAECs治疗，D组注射等量生理盐水。D组进行PRP治疗，C组注射等量生理盐水。E组进行hAECs联合PRP治疗。观察比较两组大鼠子宫内膜组织基质金属蛋白酶-9（MMP-9）、盘状结构域受体2（DDR2）蛋白表达水平、PI3K/Akt/mTOR通路相关mRNA表达量、血清白细胞介素-6（IL-6）、白细胞介素-8（IL-8）水平以及各组大鼠妊娠孕囊数量比较。结果：与A组相比，B组的子宫内膜组织MMP-9、DDR2蛋白表达水平、p-mTOR mRNA、p-Akt mRNA水平以及血清IL-6、IL-8水平比较无差异（P＞0.05）；C组、D组、E组大鼠的子宫内膜组织MMP-9、DDR2蛋白表达水平明显下降，IL-6、IL-8水平、p-mTOR mRNA、p-Akt mRNA表达升高（P＜0.05）。其中，E组大鼠MMP-9、DDR2蛋白表达水平明显高于C组、D组，IL-6、IL-8水平及p-mTOR mRNA、p-Akt mRNA表达低于C组、D组（P＜0.05）。C组、D组大鼠MMP-9、DDR2蛋白表达水平、血清IL-6、IL-8水平及p-mTOR mRNA、p-Akt mRNA表达比较（P＞0.05）。妊娠孕囊数量方面，与A组比较，B组大鼠妊娠孕囊数量。结论：hAECs联合PRP治疗可能通过上调宫腔粘连大鼠子宫内膜组织MMP-9、DDR2蛋白表达水平并下调p-mTOR mRNA、p-Akt mRNA表达，同时降低炎性因子IL-6、IL-8水平，进而提高妊娠孕囊数量，起到治疗作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the therapeutic effect of human Amniotic Epithelial cells (hAECs) combined with platelet rich Plasma (PRP) on intrauterine adhesion in rats, so as to provide a theoretical basis for optimizing the treatment of intrauterine adhesion in clinical treatment. Methods: 40 SPF female SD rats were randomly divided into blank group (group A), sham operation group (group B), hAECs group (group C), PRP group (group D) and hAECs+PRP group (group E) according to the random number table method, with 8 rats in each group. Group A did not do any operation, group B did anesthesia and open surgery, groups C, D, E used scratching method to make intrauterine adhesion model. Subsequently, groups C was treated with hAECs, and group D was injected with the same volume of normal saline. Groups D was treated with PRP, and group C was injected with the same volume of normal saline. Group E was treated with hAECs combined with PRP. The protein expression levels of MMP-9 and DDR2, related mRNA expression levels of PI3K/Akt/mTOR pathway, serum levels of IL-6 and IL-8, and the number of gestational sacs in each group were observed and compared between the two groups. Results: Compared with group A, there were no differences in the protein expression levels of MMP-9 and DDR2, p-MTOR mRNA and p-Akt mRNA levels, and serum IL-6 and IL-8 levels in group B (P>0.05). The protein expression levels of MMP-9 and DDR2 in endometrial tissues of rats in groups C, D and E were decreased, while the levels of IL-6 and IL-8, p-mTOR mRNA and p-Akt mRNA were increased (P<0.05). The protein expression levels of MMP-9 and DDR2 in group E were significantly higher than those in groups C and D, and the levels of IL-6 and IL-8, p-mTOR mRNA and p-Akt mRNA were lower than those in groups C and D (P<0.05). There were no differences in the protein expression levels of MMP-9 and DDR2, serum IL-6 and IL-8 levels, and the mRNA expression of p-mTOR and p-Akt between groups C and D (P>0.05). In terms of the number of gestational sacs, compared with group A, the number of gestational sac of rats in group B. Conclusion: hAECs combined with PRP may plays a therapeutic role by upregulation of MMP-9 and DDR2 protein expression levels and down-regulation of P-mTOR mRNA and p-Akt mRNA expression in endometrial tissue of rats with intrauterine adhesion, and decreasing the levels of inflammatory factors IL-6 and IL-8, thereby increasing the number of gestational sacs in pregnancy. |
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