| 刘 正,郑艳丽,贺云云,李 静,王 静.Snail1基因表达在妊娠期糖尿病大鼠氧化应激及肝脏损伤的作用机制[J].现代生物医学进展英文版,2023,(7):1243-1247. |
| Snail1基因表达在妊娠期糖尿病大鼠氧化应激及肝脏损伤的作用机制 |
| Mechanism of Snail1 Gene Expression in Oxidative Stress and Liver Injury in Gestational Diabetes Mellitus Rats |
| Received:October 14, 2022 Revised:November 10, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.07.008 |
| 中文关键词: Snail1基因 妊娠期糖尿病大鼠 氧化应激 肝脏损伤 |
| 英文关键词: Snail1 gene Gestational diabetes mellitus rats Oxidative stress Liver damage |
| 基金项目:陕西省重点研发计划项目（2019SF-051） |
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| 中文摘要: |
| 摘要 目的：探讨Snail1基因表达在妊娠期糖尿病大鼠氧化应激及肝脏损伤的作用机制。方法：健康成年C57BL/6雌性大鼠32只作为研究对象。采用高脂喂养联合小剂量链脲佐菌素注射的方式构建妊娠期糖尿病大鼠模型。将所有大鼠分为正常妊娠组，正常妊娠+Snail1过表达组，妊娠期糖尿病组，妊娠期糖尿病+Snail1过表达组。采用qRT-PCR检测大鼠Snail1的mRNA表达水平，并检测大鼠妊娠期体重、氧化应激指标、炎症指标和肝功能指标。结果：与正常妊娠组相比，正常妊娠+Snail1过表达组、妊娠期糖尿病组、妊娠期糖尿病+Snail1过表达组的孕鼠体重、胎鼠体重、胎盘重量、Snail1 mRNA，明显更高（P＜0.05），且妊娠期糖尿病+Snail1过表达组孕鼠体重、胎鼠体重、胎盘重量、Snail1 mRNA均显著高于正常妊娠+Snail1过表达组和妊娠期糖尿病组（P＜0.05）；与正常妊娠组相比，正常妊娠+Snail1过表达组、妊娠期糖尿病组、妊娠期糖尿病+Snail1过表达组的FBG、FINS、HOMA-IR明显更高（P＜0.05），且妊娠期糖尿病+Snail1过表达组FBG、FINS、HOMA-IR均显著高于正常妊娠+Snail1过表达组和妊娠期糖尿病组（P＜0.05）；与正常妊娠组相比，正常妊娠+Snail1过表达组、妊娠期糖尿病组、妊娠期糖尿病+Snail1过表达组的ROS、GSH-Px、MDA明显更高（P＜0.05），且妊娠期糖尿病+Snail1过表达组ROS、GSH-Px、MDA均显著高于正常妊娠+Snail1过表达组和妊娠期糖尿病组（P＜0.05）；与正常妊娠组相比，正常妊娠+Snail1过表达组、妊娠期糖尿病组、妊娠期糖尿病+Snail1过表达组的TNF-α、IL-1β、IL-18明显更高（P＜0.05），且妊娠期糖尿病+Snail1过表达组TNF-α、IL-1β、IL-18均显著高于正常妊娠+Snail1过表达组和妊娠期糖尿病组（P＜0.05）；与正常妊娠组相比，正常妊娠+Snail1过表达组、妊娠期糖尿病组、妊娠期糖尿病+Snail1过表达组的GPT、GOT、ALP明显更高（P＜0.05），且妊娠期糖尿病+Snail1过表达组GPT、GOT、ALP均显著高于正常妊娠+Snail1过表达组和妊娠期糖尿病组（P＜0.05）。结论：妊娠期糖尿病大鼠Snail1基因的表达上调可加重糖脂代谢紊乱，并激活下游氧化应激和炎症反应，进而加重大鼠肝脏损伤。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the mechanism of Snail1 gene expression in oxidative stress and liver injury in rats with gestational diabetes mellitus. Methods: Thirty-two healthy adult C57BL/6 female rats were used as the research object. The rat model of gestational diabetes mellitus was established by high-fat feeding combined with low-dose streptozotocin injection. All rats were divided into normal pregnancy group, normal pregnancy +Snail1 overexpression group, gestational diabetes mellitus group, gestational diabetes +Snail1 overexpression group. The mRNA expression level of Snail1 was detected by qRT-PCR, and the gestational weight, oxidative stress, inflammation and liver function of rats were detected. Results: Compared with the normal pregnancy group, the weight of pregnant mice, fetal weight, placenta weight and Snail1 mRNA in the normal pregnancy +Snail1 overexpression group, gestational diabetes mellitus group and gestational diabetes +Snail1 overexpression group were significantly higher(P<0.05). The gestational diabetes mellitus +Snail1 overexpression group had higher gestational diabetes mellitus +Snail1 overexpression group than the normal pregnancy +Snail1 overexpression group and gestational diabetes mellitus group(P<0.05). Compared with normal pregnancy group, FBG, FINS and HOMA-IR were significantly higher in normal pregnancy +Snail1 overexpression group, gestational diabetes mellitus group and gestational diabetes +Snail1 overexpression group(P<0.05). FBG, FINS and HOMA-IR in gestational diabetes mellitus +Snail1 overexpression group were higher than those in normal pregnancy +Snail1 overexpression group and gestational diabetes mellitus group (P<0.05). Compared with normal pregnancy group, ROS, GSH-Px and MDA in normal pregnancy +Snail1 overexpression group, gestational diabetes mellitus group and gestational diabetes +Snail1 overexpression group were higher(P<0.05). The ROS, GSH-Px and MDA of gestational diabetes mellitus +Snail1 overexpression group were higher than those of normal pregnancy +Snail1 overexpression group and gestational diabetes mellitus group(P<0.05). Compared with normal pregnancy group, TNF-α, IL-1β and IL-18 in normal pregnancy +Snail1 overexpression group, gestational diabetes mellitus group and gestational diabetes +Snail1 overexpression group were significantly higher(P<0.05). Tnf-α, IL-1β and IL-18 in gestational diabetes +Snail1 overexpression group were higher than those in normal pregnancy +Snail1 overexpression group and gestational diabetes group(P<0.05). Compared with the normal pregnancy group, the GPT, GOT and ALP of the normal pregnancy +Snail1 overexpression group, gestational diabetes mellitus group and gestational diabetes +Snail1 overexpression group were higher(P<0.05). GPT, GOT and ALP in gestational diabetes +Snail1 overexpression group were higher than those in normal pregnancy +Snail1 overexpression group and gestational diabetes group(P<0.05). Conclusion: The up-regulation of Snail1 gene in GDM rats can aggravate the disorder of glucose and lipid metabolism, activate downstream oxidative stress and inflammation, and further aggravate liver injury in rats. |
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