| 张文强,李 慧,朱芳红,叶飞林,薛 强.丹参多酚酸盐通过SIRT3/β-catenin-PPARγ信号通路对心肌梗死大鼠的作用及机制研究[J].现代生物医学进展英文版,2023,(7):1225-1230. |
| 丹参多酚酸盐通过SIRT3/β-catenin-PPARγ信号通路对心肌梗死大鼠的作用及机制研究 |
| Effect and Mechanism of Salvianolate on Myocardial Infarction Rats through SIRT3/β-catenin-PPARγ Signaling Pathway |
| Received:November 28, 2022 Revised:December 23, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.07.005 |
| 中文关键词: 丹参多酚酸盐 心肌梗死 SIRT3/β-catenin-PPARγ 心肌损伤 心功能 |
| 英文关键词: Salvianolate Myocardial infarction SIRT3/β-catenin-PPARγ Myocardial injury Cardiac function |
| 基金项目:国家自然科学基金项目（82104718） |
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| 中文摘要: |
| 摘要 目的：通过制备心肌梗死大鼠模型，基于SIRT3/β-catenin-PPARγ信号通路探讨丹参多酚酸盐对心肌梗死大鼠的作用及相关机制，为将丹参多酚酸盐应用于心肌梗死治疗积累理论基础。方法：选取SPF级健康SD大鼠60只，随机分为假手术组（A组）、模型组（B组）、丹参多酚酸注射低剂量组（C组）丹参多酚酸注射高剂量组（D组）。B、C、D组大鼠制备为心肌梗死模型，A组大鼠仅进行假手术操作。B、C、D三组大鼠均接受丹参多酚酸盐注射治疗，A组大鼠注射等量生理盐水。比较各组大鼠心动图指标、血流动力学参数、心肌损伤相关指标、SIRT3/β-catenin-PPARγ信号通路相关蛋白及mRNA相对表达量。结果：干预后，B、C、D组大鼠的LVEF、LVFS和dP/dt max、dP/dt min均下降，且C、D组高于B组，D组高于C组；同时B、C、D组大鼠LVEDD高于A组，C、D组低于B组且D组低于C组。B、C、D组大鼠CK、CK-MB、LDH水平均高于A组，同时C、D组均低于B组，且D组低于C组；干预后，B、C、D组大鼠IL-1β、IL-6、TNF-α水平均高于A组，同时C、D组均低于B组，且D组低于C组；干预后，B、C、D组大鼠SIRT3、β-catenin蛋白表达量及mRNA表达量均高于A组，同时C、D组大鼠高于B组且D组高于C组；同时B、C、D组PPARγ蛋白表达量及mRNA表达量均高于A组，且C、D组低于B组，D组低于C组（P＜0.05）。结论：丹参多酚酸盐能够有效改善心肌梗死大鼠的心功能，缓解心肌损伤，其机制可能与调控SIRT3/β-catenin-PPARγ信号通路、下调炎性因子水平相关，且高剂量丹参多酚酸盐的治疗效果更优。 |
| 英文摘要: |
| ABSTRACT Objective: To establish a rat model of myocardial infarction, and explore the effect of salvianolate on myocardial infarction and its related mechanism based on SIRT3/β-catenin-PPARγ signaling pathway, which can provide the theoretical basis for the application of salvianolate in the treatment of myocardial infarction. Methods: 60 SPF healthy SD rats were randomly divided into sham operation group (group A), model group (group B), low-dose group (group C) and high-dose group (group D). Rats in groups B, C and D were made into myocardial infarction models, while rats in group A only underwent sham operation. Rats in groups B, C and D were treated by injection of salvianolate, while rats in group A were injected with the same amount of normal saline. The indexes of echocardiography, hemodynamic parameters, myocardial injury related indexes, SIRT3/β-catenin-PPARγ signal pathway related protein and mRNA relative expression were compared in each group. Results: After intervention, the LVEF, LVFS, dP/dt max, dP/dt min of rats in groups B, C and D decreased, and those in groups C and D were higher than those in group B, and those in group D were higher than those in group C. Meanwhile, LVEDD was higher in groups B, C and D than in group A, lower in groups C and D than in group B, and lower in group D than in group C. The levels of CK, CK-MB and LDH of rats in groups B, C and D were higher than those in group A, while those in groups C and D were lower than those in group B, and group D was lower than group C. After intervention, the levels of IL-1β, IL-6 and TNF-α in groups B, C and D were higher than those in group A, while those in groups C and D were lower than those in group B, and those in group D were lower than those in group C. After intervention, the protein and mRNA expression levels of SIRT3 and β-catenin in groups B, C and D were higher than those in group A, and those in groups C and D were higher than those in group B, and those in group D were higher than those in group C. At the same time, the protein expression and mRNA expression of PPARγ in groups B, C and D were higher than those in group A, and those in groups C and D were lower than those in group B, and those in group D were lower than those in group C(P<0.05). Conclusion: Salvianolate can effectively improve the cardiac function and alleviate myocardial injury in rats with myocardial infarction, and its mechanism may be related to regulating SIRT3/β-catenin-PPARγ signal pathway and down-regulating the level of inflammatory factors, and the therapeutic effect of high-dose Salvianolate is better. |
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