贺艳丽,王运萍,綦春蕾,赵淑华,李玲霞,周福兴,张 潍.ω-3脂肪酸对人滋养层细胞侵袭和血管生成的影响[J].现代生物医学进展英文版,2023,(6):1010-1016. |
ω-3脂肪酸对人滋养层细胞侵袭和血管生成的影响 |
Effects of Omega-3 Fatty Acids on the Invasion and Angiogenesis of Human Trophoblast Cells |
Received:August 18, 2022 Revised:September 15, 2022 |
DOI:10.13241/j.cnki.pmb.2023.06.003 |
中文关键词: ω-3脂肪酸 滋养层细胞 侵袭 血管生成 三结构域蛋白22 信号转导和转录激活因子1 |
英文关键词: ω-3 fatty acids Trophoblast cells Invasion Angiogenesis Tripartite motif-containing 22 Signal transducer and activator of transcription 1 |
基金项目:国家自然科学基金青年基金项目(82002735) |
|
Hits: 832 |
Download times: 465 |
中文摘要: |
摘要 目的:探讨ω-3脂肪酸对人滋养层细胞(HTR-8/SVneo)侵袭和血管生成的影响。方法:本实验设置了不同浓度二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)处理组,依次为0、1、50和100 μmol/L EPA组;0、1、50和100 μmol/L DHA组。各组HTR-8/SVneo细胞分别用相应浓度的EPA和DHA培养48 h。然后通过CCK-8法检测细胞增殖,Matrigel Transwell实验检测细胞侵袭。使用EPA和DHA处理的HTR-8/SVneo细胞的上清液培养人脐静脉内皮细胞(HUVEC)6 h,然后检测HUVEC的小管形成能力。通过qRT-PCR和Western blot检测HTR-8/SVneo细胞中三结构域蛋白22(TRIM22)、信号转导和转录激活因子1(STAT1)、p-STAT1(Tyr701)、基质金属蛋白酶2(MMP2)、MMP9和VEGF的表达。结果:与0 μmol/L EPA组或0 μmol/L DHA组相比,50 μmol/L EPA组、100 μmol/L EPA组、50 μmol/L DHA组和100 μmol/L DHA组的OD450nm 、侵袭细胞数量、MMP2和MMP9的蛋白相对表达量均升高(P<0.05)。与0 μmol/L EPA组或0 μmol/L DHA组相比,50 μmol/L EPA组、100 μmol/L EPA组、50 μmol/L DHA组和100 μmol/L DHA组的相对小管长度和VEGF蛋白相对表达量均升高(P<0.05)。与0 μmol/L EPA组或0 μmol/L DHA组相比,50 μmol/L EPA组、100 μmol/L EPA组、50 μmol/L DHA组和100 μmol/L DHA组的TRIM22 mRNA和蛋白相对表达量均升高,而STAT1 mRNA相对表达量和p-STAT1 (Tyr701)蛋白相对表达量均降低(P<0.05)。结论:ω-3脂肪酸处理可促进滋养层细胞的侵袭性和血管生成,其机制可能与TRIM22的上调和STAT1活性的抑制有关。 |
英文摘要: |
ABSTRACT Objective: To investigate the effects of omega-3 fatty acids on the invasion and angiogenesis of human trophoblast cells (HTR-8/SVneo). Methods: Different concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) groups were set up: 0 μmol/L EPA group, 1 μmol/L EPA group, 50 μmol/L EPA group and 100 μmol/L EPA group; 0 μmol/L DHA group, 1 μmol/L DHA group, 50 μmol/L DHA group and 100 μmol/L DHA group. The HTR-8/SVneo cells in each group were cultured with corresponding concentrations of EPA and DHA for 48 h. Then the cell proliferation was detected by the CCK-8 method, and the cell invasion was detected by the Matrigel Transwell test. Human umbilical vein endothelial cells (HUVEC) were cultured for 6 h with the supernatant of HTR-8/SVneo cells treated with EPA and DHA, and then the tubule forming ability of HUVEC was detected. The expression level of tripartite motif-containing 22 (TRIM22), signal transducer and activator of transcription 1 (STAT1), p-STAT1 (Tyr701), matrix metalloproteinase 2 (MMP2), MMP9, MMP9 and VEGF in HTR-8/SVneo cells were detected by qRT-PCR and Western blot. Results: Compared with the 0 μmol/L EPA group or 0 μmol/L DHA group, OD450 nm , number of invaded cells, and relative protein expression level of MMP2 and MMP9 in the 50 μmol/L EPA group, 100 μmol/L EPA group, 50 μmol/L DHA group and 100 μmol/L DHA group were increased (P<0.05). Compared with the 0 μmol/L EPA group or 0 μmol/L DHA group, the relative tubule length and the relative expression of VEGF protein in the 50 μmol/L EPA group, 100 μmol/L EPA group, 50 μmol/L DHA group and 100 μmol/L DHA group were increased (P<0.05). Compared with the 0 μmol/L EPA group or 0 μmol/L DHA group, the relative expression levels of TRIM22 mRNA and protein in the 50 μmol/L EPA group, 100 μmol/L EPA group, 50 μmol/L DHA group and 100 μmol/L DHA group were increased, while the relative expression of STAT1 mRNA and p-STAT1 (Tyr701) protein were decreased (P<0.05). Conclusion: Omega-3 fatty acid treatment can promote the invasiveness and angiogenesis of trophoblast cells. The mechanism may be related to the up-regulation of TRIM22 and the inhibition of STAT1 activity. |
View Full Text
View/Add Comment Download reader |
Close |
|
|
|