| 孙菁菁,江 静,余 静,张东宪,王小木.间充质干细胞联合铂类对肝癌大鼠抗癌效果及对免疫功能、细胞凋亡的影响[J].现代生物医学进展英文版,2023,(5):835-839. |
| 间充质干细胞联合铂类对肝癌大鼠抗癌效果及对免疫功能、细胞凋亡的影响 |
| Effects of Mesenchymal Stem Cells Combined with Platinum on Anticancer Effect, Immune Function and Apoptosis of Liver Cancer Rats |
| Received:June 24, 2022 Revised:July 19, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.05.007 |
| 中文关键词: 肝癌 间充质干细胞 顺铂 免疫功能 细胞凋亡 |
| 英文关键词: Liver cancer Bone marrow mesenchymal stem cells DDP Immunity Apoptosis |
| 基金项目:陕西省中医药管理局委托办事经费项目（2021-ZZ-LC012） |
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| 中文摘要: |
| 摘要 目的：探讨骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）联合顺铂（cis-diamminodichloroplatinum Ⅱ dichloride，DDP）对肝癌大鼠抗癌效果及其免疫功能和细胞凋亡水平的影响。方法：将40只雄性Wistar大鼠随机分为空白对照组、模型组、DDP组和联合组，各10只。除空白对照组外，其余均采用二乙基亚硝胺饲喂法建立肝癌模型。造模成功后，空白对照组和模型组大鼠静腹腔注射生理盐水并经尾静脉注射DMEM培养液，DDP组大鼠经腹腔注射DDP溶液并经尾静脉注射DMEM培养液，联合组大鼠经腹腔注射DDP溶液并经尾静脉注射BMSCs悬液。移植BMSCs两周后处死大鼠，称量肝脏质量和体质量，采用酶联免疫吸附法检测各组大鼠外周血中白细胞介素-2（interleukin-2，IL-2）、IL-8、肿瘤坏死因子α（tumor necrosis factor α，TNF-α）、血管内皮生长因子（vascular endothelial growth factor，VEGF）和低氧诱导因子-1α（hypoxia-inducible factor-1α，HIF-1α）水平，采用TUNEL技术检测肝细胞凋亡指数。结果：（1）模型组肝脏质量、肝脏质量/体质量均显著大于其他各组，体质量显著小于其他各组（P<0.05）。联合组肝脏质量、肝脏质量/体质量均显著显著小于模型组和DDP组，体质量显著大于模型组和DDP组（P<0.05）。（2）DDP组和联合组大鼠治疗后血清IL-2水平显著升高，IL-8和TNF-α水平显著降低（P<0.05）。联合组大鼠治疗后血清IL-2水平显著高于DDP组，IL-8和TNF-α水平显著低于DDP组（P<0.05）。（3）DDP组和联合组大鼠治疗后血清VEGF和HIF-1α水平显著降低，且显著低于模型组（P<0.05）。联合组大鼠治疗后血清VEGF和HIF-1α水平显著低于DDP组（P<0.05）。（4）联合组和DDP组肝癌细胞凋亡指数显著高于模型组，且联合组也显著高于DDP组（P<0.05）。结论：BMSCs联合DDP能够显著改善肝癌大鼠免疫功能，抑制肿瘤血管生成，促进肝癌细胞凋亡，效果优于DDP单药。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of BMSCs combined with cisplatin (DDP) on the anticancer effect, immune function and apoptosis of liver cancer rats. Methods: Forty male Wistar rats were randomly divided into blank control, model, DDP, and combination groups, with 10 rats each. Except for the blank control group, other rats were fed with diethylnitrosamine to establish liver cancer models. After successful modeling, rats in the blank control group and the model group were injected with normal saline intraperitoneally and DMEM culture solution through the tail vein, rats in the DDP group were injected with DDP solution intraperitoneally and DMEM culture solution through the tail vein, and rats in the combined group were injected with DDP solution intraperitoneally and BMSCs suspension through the tail vein. Two weeks after BMSCs transplantation, the rats were killed, the liver mass and body mass were weighed, and the levels of IL-2, IL-8 and TNF-α, VEGF and HIF-1α in peripheral blood of rats in each group were detected by enzyme-linked immunosorbent assay. TUNEL technique was used to detect hepatocyte apoptosis index. Results: (1) The liver mass, liver mass / body mass of the model group were significantly higher than those of other groups, and the body mass was significantly lower than that of other groups (P<0.05). The liver mass, liver mass/body mass of the combined group were significantly lower than that of the model group and DDP group, and the body mass was significantly higher than that of the model group and DDP group(P<0.05). (2) The levels of serum IL-2, IL-8 and TNF-α in DDP group and combined group increased significantly after treatment decreased significantly (P<0.05). The levels of serum IL-2, IL-8 and TNF-α in the combined group were significantly higher than those in the DDP group after treatment The level was significantly lower than that in DDP group(P<0.05). (3) Serum levels of VEGF and HIF-1α in DDP group and combined group after treatment were significantly lower than those of the simple model(P<0.05). Serum levels of VEGF and HIF-1α in the combined group after treatment were significantly lower than those in DDP group (P<0.05). (4)The apoptosis index of liver cancer cells in the combination group and DDP group was significantly higher than that in the model group, and the combination group was also significantly higher than that in the DDP group(P<0.05). Conclusion: BMSCs combined with DDP can significantly improve the immune function of liver cancer rats, inhibit tumor angiogenesis, and promote liver cancer cell apoptosis. The effect is better than that of DDP alone. |
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