| 王喜尧,王育文,齐桂云,徐洪伟,杜桂芹.TLR4、TRAF6、IL-6和IL-1β在深静脉血栓大鼠模型中的表达和意义[J].现代生物医学进展英文版,2023,(4):641-645. |
| TLR4、TRAF6、IL-6和IL-1β在深静脉血栓大鼠模型中的表达和意义 |
| Expression and Significance of TLR4, TRAF6, IL-6 and IL-1β in Rat Model of Deep Vein Thrombosis |
| Received:June 27, 2022 Revised:July 23, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.04.008 |
| 中文关键词: 深静脉血栓 TLR4 TRAF6 IL-6 IL-1β |
| 英文关键词: Deep vein thrombosis TLR4 TRAF6 IL-6 IL-1β |
| 基金项目:黑龙江省卫生健康委项目（2019-235） |
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| 中文摘要: |
| 摘要 目的：探究4个炎症因子Toll样受体4（TLR4）、TNF受体相关因子6（TRAF6）、白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）在深静脉血栓大鼠体内的表达及在深静脉血栓形成的作用。方法：将SD大鼠随机分成对照组（n=10）和模型组（n=90），采用血管钳夹结合固定制动的方式建立深静脉血栓模型，建模后分别于1 h、6 h、12 h、1 d、3 d、7 d、14 d、21 d、28 d处死，检测血栓重量/长度比值、血浆中4个炎症因子的浓度，并进行组织学观察。结果：（1）1 h、6 h、12 h的血栓重量/长度比值较低，组间无显著性差异（P>0.05）；1 d、3 d、7 d的血栓重量/长度比值维持在较高水平，组间无显著性差异（P>0.05）；14 d、21 d、28 d的血栓重量/长度比值均降低，组间无显著性差异（P>0.05）。（2）模型组中1-7 d的血栓重量/长度比值均明显高于正常组（P<0.05）。（3）模型组中1-7 d的TLR4、TRAF6、IL-6和IL-1β浓度水平均明显高于正常组（P<0.05）。（4）Pearson 相关性分析显示TLR4、TRAF6、IL-6和IL-1β浓度与血栓程度具有正相关性。结论：深静脉血栓大鼠体内的TLR4、TRAF6、IL-6和IL-1β表达上调可能在深静脉血栓形成中发挥了重要作用。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of four inflammatory factors (Toll-like receptor 4, TLR4; TNF receptor-related factor 6, TRAF6; interleukin-6, IL-6; interleukin-1β, IL-1β) in rats with deep vein thrombosis expression and role in deep vein thrombosis. Methods: SD rats were randomly divided into control group (n=10) and model group (n=90), and the deep vein thrombosis model was established by vascular clamp combined with immobilization. After modeling, the rats were sacrificed at 1 h, 6 h, 12 h, 1 d, 3 d, 7 d, 14 d, 21 d, and 28 d, respectively. The thrombus weight/length ratio and the concentration of 4 inflammatory factors in plasma were detected, and histological observation was performed. Results: (1) The thrombus weight/length ratio at 1 h, 6 h, and 12 h was lower, and there was no significant difference between the groups (P>0.05); the thrombus weight/length ratio at 1 d, 3 d, and 7 d remained at a high level, and there was no significant difference between the groups. The thrombus weight/length ratio decreased on 14 d, 21 d, and 28 d, and there was no significant difference between groups (P>0.05). (2) The thrombus weight/length ratios in the model group from 1 to 7 days were significantly higher than those in the normal group (P<0.05). (3) The concentrations of TLR4, TRAF6, IL-6 and IL-1β in the model group from 1 to 7 days were significantly higher than those in the normal group (P<0.05). (4) Pearson correlation analysis showed that the concentrations of TLR4, TRAF6, IL-6 and IL-1β were positively correlated with the degree of thrombosis. Conclusion: Up-regulation of TLR4, TRAF6, IL-6 and IL-1β in rats with deep vein thrombosis may play an important role in deep vein thrombosis. |
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