| 黄婉莹,张永芳,王 昊,赵兰雪,宋成寰,张 瑞.梓醇对Aβ损伤SH-SY5Y细胞BDNF表达的影响及机制研究[J].现代生物医学进展英文版,2023,(4):601-606. |
| 梓醇对Aβ损伤SH-SY5Y细胞BDNF表达的影响及机制研究 |
| The Effect of Catalpol on BDNF Expression in Aβ Intoxicated SH-SY5Y Cells and Mechanism Research |
| Received:July 13, 2022 Revised:August 10, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.04.001 |
| 中文关键词: 梓醇 阿尔茨海默症 脑源性神经营养因子 cAMP反应元件结合蛋白 |
| 英文关键词: Catalpol Alzheimer's disease (AD) Brain-derived neurotrophic factor (BDNF) cAMP response element binding protein (CREB) |
| 基金项目:国家自然科学基金项目（82003719;81973297；82073836；81872841）；上海市科委重点项目(19JC1413100)；上海交通大学"医学转化交叉基金"项目(ZH2018ZDA23) |
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| 中文摘要: |
| 摘要 目的：探讨梓醇对β-淀粉样肽（β-amyloid, Aβ）损伤SH-SY5Y细胞脑源性神经营养因子（brain-derived neurotrophic factor, BDNF）表达的影响及调节BDNF表达的机制。方法：以全反式维甲酸诱导分化的神经母细胞瘤细胞SH-SY5Y为研究对象，研究梓醇对Aβ 损伤细胞的作用。采用MTT实验测定细胞存活率，ELISA测定BDNF的含量，Western blot检测转录因子cAMP反应元件结合蛋白（cAMP response element binding protein, CREB）及其活化形式磷酸化CREB（pCREB）表达量，RT-PCR测定BDNF mRNA及转录子的表达水平；用RNA干扰的方法阻断CREB表达后，用RT-PCR测定BDNF mRNA表达量的变化。结果：梓醇预保护提高Aβ损伤SH-SY5Y细胞的存活率，显著增加细胞培养上清中BDNF含量和胞内BDNF mRNA水平，促进BDNF转录子IV及其关键调节转录因子pCREB的表达，干扰CREB表达后，梓醇上调BDNF mRNA表达的作用部分消失。结论：梓醇可能通过上调CREB磷酸化促进BDNF的表达，从而发挥神经保护作用。 |
| 英文摘要: |
| ABSTRACT Objective: Discuss the effect of catalpol on brain-derived neurotrophic factor (BDNF) expression in β-Amyloid peptide (Aβ) intoxicated SH-SY5Y cells and the mechanism of regulating BDNF expression. Methods: The SH-SY5Y neuroblastoma cells induced by all-trans retinoic acid were used as the research object, and the effect of catalpol on Aβ-injured cells was studied. The cell survival rate was determined by MTT assay, and the content of BDNF was determined by ELISA. The Western blot was used to detect the expression levels of transcription factors cAMP response element binding protein (CREB) and phosphorylated CREB (pCREB, activated form of CREB). The mRNA expression levels of BDNF and its series of transcripts were determined by RT-PCR. CREB expression was blocked by RNA interference and the expression of BDNF mRNA was determined by RT-PCR. Results: Catalpol pre-protection increased the survival rate of SH-SY5Y cells after Aβ injury, significantly increased the content of BDNF in cell culture supernatant and intracellular BDNF mRNA level, promoted the expression of BDNF transcription factor IV and its key regulatory transcription factor pCREB. After interfered with CREB expression, the upregulation effect of catalpol on BDNF mRNA expression partially disappeared. Conclusion: Catalpol may play a neuroprotective role by upregulating the phosphorylation of CREB and promoting the expression of BDNF. |
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