| 李迎楼,王诗维,董茜茜,王珍珍,杨建军,郭 晶.纺壳聚糖聚己内酯纳米纤维膜对鼠骨髓间充质干细胞成牙功能影响[J].现代生物医学进展英文版,2023,(3):423-427. |
| 纺壳聚糖聚己内酯纳米纤维膜对鼠骨髓间充质干细胞成牙功能影响 |
| Effect of Spinning Chitosan Polycaprolactone Nanofiber Membrane on Dental Function of Rat Bone Marrow Mesenchymal Stem Cells |
| Received:May 27, 2022 Revised:June 24, 2022 |
| DOI：10.13241/j.cnki.pmb.2023.03.005 |
| 中文关键词: 纺壳聚糖 聚己内酯纳米纤维膜 骨髓间充质干细胞 细胞增殖 碱性磷酸酶 |
| 英文关键词: Spinning chitosan Polycaprolactone nanofiber membrane Bone marrow mesenchymal stem cells Cell proliferation Alkaline phosphatase |
| 基金项目:国家自然科学基金青年项目（82008275） |
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| 中文摘要: |
| 摘要 目的：探讨纺壳聚糖聚己内酯纳米纤维膜对鼠骨髓间充质干细胞（bone marrow mesenchymal stem cell，BMSC）成牙功能影响。方法：从SD大鼠分离BMSCs，然后随机分为两组，一组与纺壳聚糖聚己内酯纳米纤维膜进行共培养（共培养组）；另一组不进行共培养，直接在细胞板内培养（对照组）。检测细胞增殖指数、细胞钙含量、碱性磷酸酶活性与成骨相关基因表达水平。结果：BMSCs细胞形态较为单一，呈纤维样、旋涡状梭形、生长，长期培养可见细胞成片生长并相互融合。与共培养24 h相比，共培养48 h后共培养组细胞增殖指数、钙含量、碱性磷酸酶活性以及O成骨相关基因CN、Col1、Runx2等相对表达水平均显著增加（P<0.05）。共培养24 h与48 h后，共培养组的细胞增殖指数、钙含量、碱性磷酸酶活性和骨钙素（OCN）、Ⅰ型胶原α1（Col1）、Runt相关转录因子2（Runx2）等成骨相关基因相对表达水平都显著高于对照组（P<0.05）。结论：纺壳聚糖聚己内酯纳米纤维膜在BMSCs中的应用能促进细胞增殖，提高碱性磷酸酶活性与钙含量，促进成骨相关基因的表达，从而发挥成牙功能。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effects of spinning chitosan polycaprolactone nanofiber membrane on the dental function of BMSC. Methods: BMSCs were isolated from SD rats, and then randomly divided into two groups. The one group were co-cultured with spinning chitosan polycaprolactone nanofiber membrane (co-culture group); the other group were not co-cultured and directly in the cell in-plate culture (control group). Detected cell proliferation index, cell calcium content, alkaline phosphatase activity and osteogenic-related gene expression levels. Results: The cell morphology of BMSCs were relatively simple, showing fibrous, vortex-like spindle shape and growth. Long-term culture showed that the cells grew in sheets and merged with each other. Compared with co-culture for 24 h, the cell proliferation index, calcium content, alkaline phosphatase activity and relative expression levels of O osteogenesis-related genes CN, Col1 and Runx2 in the co-culture group were significantly increased after co-culture for 48 h (P<0.05). After 24 h and 48 h of co-culture, the cell proliferation index, calcium content, alkaline phosphatase activity and osteocalcin (OCN), type I collagen α1 (Col1), Runt-related transcription factor 2 (Runx2) relative expression levels of related osteogenesis genes in the co-culture group were significantly higher than those in the control group(P<0.05). Conclusion: The application of spinning chitosan polycaprolactone nanofiber membranes in BMSCs can promote cell proliferation, increase alkaline phosphatase activity and calcium content, and promote the expression of osteogenic genes, thereby exerting the function of dental formation. |
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