| 常 乐,赵轶男,宋 瑶,康飞科,孙 振.IGF-1联合BMP-2对糖尿病合并骨质疏松股骨骨折大鼠中的骨折愈合影响分析[J].现代生物医学进展英文版,2022,(24):4627-4631. |
| IGF-1联合BMP-2对糖尿病合并骨质疏松股骨骨折大鼠中的骨折愈合影响分析 |
| Effect of IGF-1 Combined with BMP-2 on Fracture Healing in Diabetic Rats with Osteoporotic Femoral Fractures |
| Received:August 10, 2022 Revised:August 30, 2022 |
| DOI：10.13241/j.cnki.pmb.2022.24.005 |
| 中文关键词: IGF-1 BMP-2 糖尿病 骨质疏松 股骨骨折 骨折愈合 |
| 英文关键词: IGF-1 BMP - 2 Diabetes Osteoporosis Fracture of femur Fracture healing |
| 基金项目:国家自然科学基金青年项目（82002348） |
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| 中文摘要: |
| 摘要 目的：探讨胰岛素生长因子-1（insulin-like growth factor, IGF-1）联合骨形态发生蛋白（bone morphogenetic protein，BMP）-2对糖尿病合并骨质疏松股骨骨折大鼠中的骨折愈合影响。方法：8周龄雌性SD大鼠60只，饲养一周后根据随机数字表法进行分组，每组12只，之后进行造模，造模成功50只，成功率为83.33 %。将其分为5组，包括正常组（n=10），糖尿病+卵巢切除+骨折组（n=9），糖尿病+卵巢切除+骨折+ IGF-1组（骨折处注射IGF 30 μg/kg，n=11），糖尿病+卵巢切除+骨折+BMP-2组（骨折处注射100 μL BMP-2基因慢病毒1×108，n=10）、糖尿病+卵巢切除+骨折+IGF-1+BMP-2组（参照上述，n=10），其余进行等剂量溶剂注射，均连续注射两天。分组处理6周后，观察5组大鼠的一般情况。对比5组大鼠的血清钙、骨钙素、碱性磷酸酶水平，对比5组的最大应力、最大载荷及刚度，检测5组大鼠组织中的IGF-1、BMP-2及TGF-β1 mRNA表达水平。结果：A组大鼠无明显异常反应，大鼠体重逐渐增加，大小便、饮食均正常，毛色光亮；B、C、D、E组大鼠精神萎靡、反应迟缓、毛色光亮性较差、体重无明显减轻或增加、大鼠的饮水量、饮食增加，多尿症状较为明显。5组大鼠的血清钙、骨钙素、碱性磷酸酶水平：B组结论：IGF-1联合BMP-2可促进糖尿病合并骨质疏松股骨骨折大鼠中的骨折愈合。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the effect of IGF-1 combined with BMP-2 on fracture healing in diabetic rats with osteoporotic femoral fractures. Methods: Sixty 8-week-old female SD rats were divided into groups according to the random number table method after one week of feeding, with 12 rats in each group. After that, membrane was constructed, and 50 rats were successfully constructed, with the success rate of 83.33%, which were divided into 5 groups, including normal group (n=10), diabetes + ovariectomy + fracture group (n=9), diabetes + ovariectomy + fracture + IGF-1 group (IGF 30 μg/kg was injected at the fracture site, n=11). Diabetes + ovariectomy + fracture + BMP-2 group (100 μL BMP-2 gene lentivirus 1×108 was injected into the fracture site, n=10), diabetes + ovariectomy + fracture + IGF-1 + BMP-2 group (refer to the above, n=10), and the rest were injected with equal doses of solvent, all of which were injected for two consecutive days. After 6 weeks of group treatment, the levels of serum calcium, osteocalcin and alkaline phosphatase in the 5 groups were detected and compared. The maximum stress, maximum load and stiffness of the 5 groups were compared. The expression levels of IGF-1, BMP-2 and TGF-β1 mRNA in the 5 groups were detected. Results: The rats in group A had no obvious abnormal reaction, the weight of the rats gradually increased, the urine and feces, diet were normal, and the fur color was bright. The rats in groups B, C, D and E had listlessness, delayed reaction, poor color brightness, no significant weight loss or increase, increased water intake and diet, and more obvious polyuria. The levels of serum calcium, osteocalcin and alkaline phosphatase in the 5 groups were as follows: group B < group C < group D < group E < group A (P<0.05). The BMD, maximum stress, maximum load and stiffness of rats in the five groups were as follows: group B < group C < group D < group E < group A (P<0.05). The expression levels of IGF-1 mrna, BMP-2 mrna and TGF-β1 mrna in the 5 groups were as follows: group B < group C < group D < group E < group A (P<0.05). Conclusion: IGF-1 combined with BMP-2 can promote fracture healing in diabetic rats with osteoporotic femoral fractures. |
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