| 何悦双,刘 姣,石丹宁,杨佳迪,尤凤鸣,赵丕文.GPER介导的丹参酮ⅡA抗三阴性乳腺癌细胞MDA-MB-231迁移作用机制研究[J].现代生物医学进展英文版,2022,(24):4601-4605. |
| GPER介导的丹参酮ⅡA抗三阴性乳腺癌细胞MDA-MB-231迁移作用机制研究 |
| Investigation of Anti-migration Effect of Tanshinone ⅡA Mediated by GPER on Triple-negative Breast Cancer Cells MDA-MB-231 |
| Received:March 28, 2022 Revised:April 24, 2022 |
| DOI：10.13241/j.cnki.pmb.2022.24.001 |
| 中文关键词: 三阴性乳腺癌 丹参酮ⅡA G蛋白偶联雌激素受体 细胞迁移 基质金属蛋白酶9 |
| 英文关键词: Triple-negative breast cancer Tanshinone ⅡA GPER Cell migration MMP-9 |
| 基金项目:国家自然科学基金项目（81673764） |
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| 中文摘要: |
| 摘要 目的：基于体外细胞实验，探索丹参酮ⅡA对三阴性乳腺癌细胞MDA-MB-231迁移的抑制作用及其分子机制。方法：选取三阴性乳腺癌细胞MDA-MB-231，利用细胞增殖实验检测丹参酮ⅡA对MDA-MB-231细胞增殖的作用，并筛选适宜的药物浓度；应用划痕实验检测丹参酮ⅡA对MDA-MB-231细胞迁移率的影响；Western Blot法检测丹参酮ⅡA对G蛋白偶联雌激素受体（G protein-coupled estrogen receptor，GPER）及基质金属蛋白酶9（Matrix metalloprotein-9，MMP-9）表达的影响。结果：细胞增殖实验结果显示，丹参酮ⅡA可以抑制MDA-MB-231细胞增殖，且呈剂量依赖性（P＜0.05）；划痕实验结果显示，丹参酮ⅡA可以抑制MDA-MB-231细胞迁移，且呈剂量依赖性（P＜0.01），加入GPER特异性抑制剂G15后迁移率有所上升（P＜0.01）。Western Blot结果显示，丹参酮ⅡA可以显著下调GPER和MMP-9蛋白的表达水平并呈剂量依赖性（P＜0.05），加入GPER特异性抑制剂G15后，MMP-9表达有所上升（P＜0.01）。结论：丹参酮ⅡA可以抑制三阴性乳腺癌细胞MDA-MB-231迁移，其机制可能与抑制GPER介导的MMP-9表达相关。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the anti-migration effect of Tanshinone ⅡA on triple negative breast cancer cells MDA-MB-231 and its molecular mechanism based on in vitro assays. Methods: Triple negative breast cancer cells MDA-MB-231 were selected, and the effect of Tanshinone ⅡA on the proliferation of MDA-MB-231 cells was detected by cell proliferation assay. In addition, the optimal drug concentration was screened. The effect of Tanshinone ⅡA on the migration rate was detected by Wound-Healing assay. Western blot was performed to detect the expression of G protein-coupled estrogen receptor (GPER) and Matrix metalloprotein-9 (MMP-9). Results: The results of cell proliferation assay showed that Tanshinone ⅡA could inhibit the proliferation of MDA-MB-231 cells in a dose-dependent manner (P<0.05). Wound-Healing assay showed that the Tanshinone ⅡA could inhibit the migration rate in a dose-dependent manner (P<0.01). Besides, the migration rate increased after administration of GPER-specific inhibitor, G15 (P<0.01). Western Blot showed that Tanshinone ⅡA significantly downregulated expressions of GPER and MMP-9 proteins in a dose-dependent manner (P<0.05). The expression of MMP-9 increased after administration of GPER-specific inhibitor G15 (P<0.01). Conclusion: Tanshinone ⅡA could inhibit the migration of triple-negative breast cancer cell MDA-MB-231, and the mechanism may be related to the inhibition of GPER-mediated MMP-9 expression. |
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