| 史凡祺,王媛媛,郑 鑫,刘世全,甄瑞鑫.HGF调控β-catenin信号通路对家兔股骨颈骨折修复的影响[J].现代生物医学进展英文版,2022,(20):3834-3839. |
| HGF调控β-catenin信号通路对家兔股骨颈骨折修复的影响 |
| Effects of HGF Regulation of β-catenin Signaling Pathway on the Repair of Femoral Neck Fractures in Rabbits |
| Received:March 24, 2022 Revised:April 18, 2022 |
| DOI：10.13241/j.cnki.pmb.2022.20.007 |
| 中文关键词: 肝细胞生长因子 Wnt/β-catenin通路 股骨颈骨折 家兔 骨折修复 |
| 英文关键词: Hepatocyte growth factor Wnt/β-catenin signaling pathway Femoral neck fractures Rabbits Fracture repair |
| 基金项目:承德市基础研究项目（201904A102） |
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| 中文摘要: |
| 摘要 目的：探究HGF调控β-catenin信号通路对家兔股骨颈骨折修复的作用和影响机制。方法：15只股骨颈骨折家兔动物模型随机分为半合成细胞外基质样水凝胶组（sECM组）、sECmM+HGF组和脱钙骨基质组（DBM组），自体右侧作为实验侧，左侧作为对照侧，对比各组家兔一般情况、骨折愈合质量评分骨痂最大载荷、挠度和刚度、BMSC细胞中BMP-2、TGF-β1、PDGF、bFGF mRNA表达水平和COL-I、Wnt5a、β-catenin和Lef-1蛋白表达水平。结果：术后各周sECM+HGF组和DBM组实验侧骨折愈合质量评分高于对照侧（P<0.05），且高于同期sECM组实验侧（P<0.05）。术后8周，sECM+HGF组和DBM组实验侧骨痂最大荷载、挠度和刚度均优于对照侧（P<0.05），且优于sECM组实验侧（P<0.05）。术后2周及4周，sECM+HGF组和DBM组实验侧BMP-2、TGF-β1、PDGF和bFGF表达显著高于对照侧（P<0.05）。且高于同期sECM组实验侧（P<0.05），术后8周sECM+HGF组和DBM组实验侧BMP-2表达高于sECM组实验侧（P<0.05）。术后2周，sECM+HGF组和DBM组实验侧COL-I表达高于对照侧（P<0.05）。术后2周、4周sECM+HGF组和DBM组实验侧COL-I表达均显著高于同期sECM组实验侧（P<0.05）。术后8周，sECM+HGF组和DBM组实验侧wnt5a表达低于对照侧（P<0.05）且低于sECM组实验侧，β-catenin、Left-1表达显著高于对照侧（P<0.05），且高于sECM组实验侧（P<0.05）。结论：HGF通过激活Wnt/β-catenin信号通路，上调BMP-2、TGF-β1、PDGF、bFGF和COL-1的表达，显著促进股骨颈骨折家兔的骨折修复过程。 |
| 英文摘要: |
| ABSTRACT Objective: To explore the effects and influencing mechanism of HGF regulating β-catenin signaling pathway on the repair of femoral neck fractures in rabbits. Methods: 15 rabbits with femoral neck fractures were randomly divided into semisynthetic extracellular matrix like hydrogel group (sECM group), sECmM+HGF group and demineralized bone matrix group (DBM group). The autologous right side was used as the experimental side and the left side as the control side. The general condition, fracture healing quality score, the maximum load, deflection and stiffness of callus and mRNA expression levels of BMP-2, TGF-β1, PDGF and bFGF and the protein expression levels of COL-I, Wnt5a, β-catenin and Lef-1 in BMSC cells were compared among the groups. Results: The fracture healing quality scores of the experimental side in sECM+HGF group and DBM group were higher than the control side at different weeks after surgery (P<0.05), and were higher than the experimental side in sECM group (P<0.05). At 8 weeks after surgery, the maximum load, deflection and stiffness of the experimental side callus in sECM+HGF group and DBM group were better than those in the control side (P<0.05), and the indicators were better than those of the experimental side in sECM group (P<0.05). At 2 weeks and 4 weeks after surgery, the expressions of BMP-2, TGF-β1, PDGF and bFGF in the experimental side were higher than those in the control side in sECM+HGF group and DBM group (P<0.05), and the expressions were higher than those in the experimental side of sECM group (P<0.05), and the expression of BMP-2 in the experimental side of sECM+HGF group and DBM group was higher than that in the experimental side of sECM group at 8 weeks after surgery (P<0.05). At 2 weeks after surgery, the expression of COL-I in the experimental side of sECM+HGF group and DBM group was higher than that in the control side (P<0.05). The expression of COL-I in the experimental side of sECM+HGF group and DBM group at 2 and 4 weeks after surgery was higher than that in the experimental side of sECM group during the same period (P<0.05). At 8 weeks after surgery, the expression of wnt5a in the experimental side of sECM+HGF group and DBM group was lower than that in the control side (P<0.05) and was lower than that in the experimental side of sECM group, while the expressions of β-catenin and Left-1 were higher than those in the control side (P<0.05) and were higher than those in the experimental side of sECM group (P<0.05). Conclusion: HGF up-regulates the expressions of BMP-2, TGF-β1, PDGF, bFGF and COL-1, and significantly promotes the fracture repair process of rabbits with femoral neck fractures by activating the Wnt/β-catenin signaling pathway. |
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