| 钱 粒,陆舒旻,何 鑫,冯 佳,章建国.PRDX1、HMGA2、FMNL2在胃癌组织中的表达及与临床病理特征、上皮间充质转化和预后的关系研究[J].现代生物医学进展英文版,2022,(17):3367-3372. |
| PRDX1、HMGA2、FMNL2在胃癌组织中的表达及与临床病理特征、上皮间充质转化和预后的关系研究 |
| Expression of PRDX1, HMGA2 and FMNL2 in Gastric Cancer Tissues and Their Relationship Study with Clinicopathological Features, Epithelial Mesenchymal Transition and Prognosis |
| Received:March 05, 2022 Revised:March 31, 2022 |
| DOI：10.13241/j.cnki.pmb.2022.17.033 |
| 中文关键词: 胃癌 PRDX1 HMGA2 FMNL2 临床病理特征 上皮-间充质转化 预后 |
| 英文关键词: Gastric cancer PRDX1 HMGA2 FMNL2 Clinicopathological features Epithelial-mesenchymal transition Prognosis |
| 基金项目:国家自然科学基金项目（82102167） |
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| 中文摘要: |
| 摘要 目的：探讨过氧化物酶1（PRDX1）、高迁移率族蛋白A2（HMGA2）、同源形成素样蛋白2（FMNL2）在胃癌组织中的表达及与临床病理特征、上皮-间充质转化（EMT）和预后的关系。方法：选取2017年1月～2018年2月我院收治的153例胃癌患者，收集术中癌组织和癌旁组织。采用免疫组化法检测PRDX1、HMGA2、FMNL2、波形蛋白（VIM）、上皮细胞钙黏蛋白（E-cad）阳性表达情况，采用实时定量PCR（qRT-PCR）法检测PRDX1、HMGA2、FMNL2、VIM、E-cad mRNA相对表达量。分析胃癌组织中PRDX1、HMGA2、FMNL2表达与临床病理特征、EMT和预后的关系。结果：与癌旁组织比较，胃癌组织中PRDX1、HMGA2、FMNL2、VIM阳性表达率和mRNA相对表达量升高，E-cad阳性表达率和mRNA相对表达量降低（P＜0.05）。胃癌组织中PRDX1、HMGA2、FMNL2阳性表达率与患者TNM分期、淋巴结转移、远处转移有关（P＜0.05）。Pearson相关性分析结果显示，胃癌组织中PRDX1、HMGA2、FMNL2 mRNA相对表达量与VIM mRNA相对表达量呈正相关（r=0.562、0.517、0.621，P均＜0.05），与E-cad mRNA相对表达量呈负相关（r=-0.603、-0.544、-0.574，P均＜0.05）。153例胃癌患者术后3年累积生存率为68.44%（106/153）。Kaplan-Meier生存曲线分析结果显示，PRDX1、HMGA2、FMNL2阳性组术后3年累积生存率均低于阴性组（P＜0.05）。结论：胃癌组织中PRDX1、HMGA2、FMNL2表达升高，其表达与TNM分期、淋巴结转移、远处转移、EMT以及预后有关，可作为胃癌病情和预后的辅助评估指标。 |
| 英文摘要: |
| ABSTRACT Objective: To investigate the expression of peroxiredoxin 1 (PRDX1), high mobility group AT-Hook 2 (HMGA2) and formin like 2 (FMNL2) in gastric cancer tissues and the relationship with clinicopathological features, epithelial-mesenchymal transition (EMT) and prognosis. Methods: A total of 153 gastric cancer patients who were admitted to our Hospital from January 2017 to February 2018 were selected, and intraoperative cancer tissues and adjacent tissues were collected. PRDX1, HMGA2, FMNL2, vimentin (VIM), epithelial-cadherin (E-cad) positive expression were detected by immunohistochemistry, and PRDX1, HMGA2, FMNL2, VIM, E-cad relative expression amount were detected by real time quantitative PCR (qRT-PCR) method. The relationship between PRDX1, HMGA2 and FMNL2 expression in gastric cancer tissues and their clinicopathological features, EMT and prognosis were analyzed. Results: Compared with adjacent tissues, the positive expression rate and relative expression amount of PRDX1, HMGA2, FMNL2 and VIM in gastric cancer tissues increased, while the positive expression rate and relative expression amount of E-cad decreased (P＜0.05). The positive expression rates of PRDX1, HMGA2 and FMNL2 in gastric cancer tissues were related to TNM stage, lymph node metastasis and distant metastasis (P＜0.05). Pearson correlation analysis showed that the relative expression amount of PRDX1, HMGA2 and FMNL2 mRNA in gastric cancer tissues were positively correlated with the relative expression amount of VIM mRNA (r=0.562, 0.517, 0.621, all P＜0.05), and negatively correlated with the relative expression amount of E-cad mRNA (r=-0.603, -0.544, -0.574, all P＜0.05). The 3-year cumulative survival rate of 153 gastric cancer patients was 68.44% (106/153). Kaplan-Meier survival curve analysis showed that the 3-year cumulative survival rate of PRDX1, HMGA2 and FMNL2 positive group were lower than those of negative group (P＜0.05). Conclusion: The expression of PRDX1, HMGA2 and FMNL2 increased in gastric cancer tissues, which is related to TNM stage, lymph node metastasis, distant metastasis, EMT and prognosis. It can be used as an auxiliary evaluation index for the condition and prognosis of gastric cancer. |
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